A5022476147- Influenza Virus Research Studies
- Respiratory viral infections research
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Toxoplasma gondii Research Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Advanced Biosensing Techniques and Applications
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Microfluidic and Bio-sensing Technologies
- Electrospun Nanofibers in Biomedical Applications
- Breast Cancer Treatment Studies
- Wound Healing and Treatments
- Photonic and Optical Devices
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Contact Dermatitis and Allergies
- Multiple and Secondary Primary Cancers
- Animal Virus Infections Studies
- Vaccine Coverage and Hesitancy
- Animal Disease Management and Epidemiology
- Protein purification and stability
Southern Medical University
2025
Nanfang Hospital
2025
Hunan Provincial Center for Disease Control and Prevention
2017-2024
Southeast University
2023-2024
National Taiwan University
2023
Development Center for Biotechnology
2013-2023
University of Cambridge
2023
University of Chinese Academy of Sciences
2023
Huashan Hospital
2023
Fudan University
2023
Background Monoclonal antibodies (mAbs) and their derivatives are the fastest expanding category of pharmaceuticals. Efficient screening generation appropriate therapeutic human important urgent issues in field medicine. The successful vitro biopanning method for antibody largely depends on highly diverse, reliable humanized CDR library. To rapidly obtain potent antibodies, we designed constructed a diverse synthetic single-chain variable fragment (scFv) library greater than giga size by...
Vascular endothelial growth factor (VEGF) is an important stimulator for angiogenesis in solid tumors. Blocking VEGF activity effective therapeutic strategy to inhibit tumor and metastasis. Avastin, a humanized monoclonal antibody recognizes VEGF, has been approved by the US Food Drug Administration. To generate potential VEGF-recognizing antibodies with better regression ability than that of we have designed systematic selection plan. From mice immunized recombinant human generated three...
Abstract Finding an interactive ligand-receptor pair is crucial to many applications, including the development of monoclonal antibodies. Biopanning, a commonly used technique for affinity screening, involves series washing steps and lengthy tedious. Here we present approach termed continuous microfluidic assortment ligands, or CMAIL, screening sorting antigen-binding single-chain variable antibody fragments (scFv) displayed on bacteriophages (phages). Phages carrying native negative charges...
To understand the prevalence of rhinovirus (RV) among acute respiratory infection (ARI) patients, 10-year ARI surveillance in multiple provinces China were conducted during 2012-2021. Of 15 645 1180 (7.54%) confirmed to have RV and 820 (69.49%) children under 5 years age. typing was performed on 527 VP1 gene sequences, species A, B, C accounted for 73.24%, 4.93%, 21.82%, respectively. Although no significant difference proportions age groups or disease severity found between species, RV-C...
Abstract Background Highly pathogenic avian influenza virus subtype H5N1 infects humans with a high fatality rate and has pandemic potential. Vaccination is the preferred approach for prevention of infection. Seasonal infection been reported to provide heterosubtypic immunity against A some extend. In this study, we used mouse model pre-exposed an H1N1 evaluated protective ability provided by single dose DNA vaccines encoding conserved proteins. Results SPF BALB/c mice were intranasally...
Human cytomegalovirus infects the majority of humanity which may lead to severe morbidity and mortality in newborns immunocompromised adults. Humoral cellular immunity are critical for controlling CMV infection. HCMV envelope glycoprotein complexes (gC I, II, III) represent major antigenic targets antiviral immune responses. The gCIII complex is comprised three glycoproteins, gH, gL, gO. In present study, DNA vaccines expressing murine homologs gO proteins were evaluated protection against...
House dust mite allergy is closely associated with allergic diseases. Blomia tropicalis species an important clinical in the tropics. The cDNA clone encoding Blo t 3, a group 3 allergen from B. tropicalis, has been isolated our laboratory.This study was designed to generate 3-specific monoclonal antibodies (mAbs) for detection, characterization and purification of this allergen.Mice were immunized intramuscularly naked plasmid DNA gene vivo electroporation. Hybridomas generated by fusion...
Human cytomegalovirus (HCMV) causes serious HCMV-related diseases in immunocompromised people. Vaccination is the most effective measure to control infection with pathogen, yet no vaccine has been licensed till now. We performed a head-to-head comparison of protective abilities multiple DNA vaccines murine model (MCMV) infection. Five were constructed. Four encoding MCMV proteins gp34 (m04), p65 (M84), helicase (M105), and immediate-early 1 protein pp89 (IE-1) , respectively, which reported...
Human cytomegalovirus (HCMV) is a ubiquitous pathogen that causes serious problems in immunocompromised or immunologically immature hosts. Vaccination the preferred approach for prevention of HCMV infection, but so far no approved vaccine available. In this study, we assessed immunogenicity and protective immunity formalin-inactivated murine (FI-MCMV) mouse model combination with adjuvants MF59, alum, chitosan. Specific-pathogen-free BALB/c mice aged 6–8 weeks were immunized twice, 3 apart,...
Vaccination is the best measure to prevent influenza pandemics. Here, we studied protective effect against heterologous viruses, including A/reassortant/NYMC X-179A (pH1N1), A/Chicken/Henan/12/2004 (H5N1), A/Chicken/Jiangsu/7/2002 (H9N2) and A/Guizhou/54/89×A/PR/8/34 (A/Guizhou-X) (H3N2), in mice first vaccinated with a DNA vaccine of haemagglutinin (HA) or neuraminidase (NA) A/PR/8/34 (PR8) then infected homologous virus. We showed that PR8 HA NA vaccination both protected lethal dose...
Strong third-harmonic generation (THG) is observed in layered violet phosphorus (VP). Polarization-resolved THG reveals its anisotropic, nonlinear optical response, which can be utilized to determine the lattice orientation optically.
Flexible lasing mode regulation is significant for optoelectronic applications, such as on-chip communication and integration. However, reversible dynamic single output still a challenge. Herein, modes tuning demonstrated in whispering gallery microcavity controlled by bias voltages. Especially coupled consisting of two microrods, applied electrical fields cause shifting one microrod, the optical field coupling process with other microrod occurs simultaneously to select distinct lasing....
Objective To analyze the genome characteristics of an avian influenza A (H9N2) virus isolated from 11-month-old infant, and to look for possible sources infection. Methods Throat swabs were collected infant with influenza-like illness in sentinel surveillance hospitals viruses using cells. The isolates identified types subtypes by method hemagglutination assay, inhibition assay fluorescence PCR. Whole sequencing was carried out. nucleic acid sequences deduced amino analyzed comparing...
Objective To construct a mutant strain of Streptococcus mutans (S.mutans) with clpC-deletion and to investigate the role clpC gene in genetic competence. Methods The fragment kanamycin resistant cassette flanked by two loxP sites were amplified PCR. The purified was cloned into pMD-19T simple vector pCKX1. pCKX1 digested ClaⅠ/EcoRⅠ, then blunted introduced lox71-KMR-lox66 obtain pCKX2 via homologous recombination. linearized SalⅠ transformed S. UA159 strain. positive strains...
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