A5052094220- Oral microbiology and periodontitis research
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Genomics and Phylogenetic Studies
- Lipoproteins and Cardiovascular Health
- Complex Systems and Time Series Analysis
- Cardiac Imaging and Diagnostics
- Metabolomics and Mass Spectrometry Studies
- Acute Myocardial Infarction Research
- Microfluidic and Bio-sensing Technologies
- Microbial metabolism and enzyme function
- Infective Endocarditis Diagnosis and Management
- Amino Acid Enzymes and Metabolism
- Financial Risk and Volatility Modeling
- Coronary Interventions and Diagnostics
- Microbial Metabolic Engineering and Bioproduction
- Advanced Chemical Sensor Technologies
- Mass Spectrometry Techniques and Applications
- Oral and gingival health research
- Oral Health Pathology and Treatment
- Lipid metabolism and disorders
- Innovative Microfluidic and Catalytic Techniques Innovation
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Metalloenzymes and iron-sulfur proteins
Ningxia Medical University
2023-2024
Valaya Alongkorn Rajabhat University
2024
Tongren University
2022-2024
Xi'an Jiaotong University
2012-2022
University of Washington
2007-2021
Guizhou University
2021
Sanya Central Hospital
2012-2018
Shanghai East Hospital
2008-2011
Beijing Jiaotong University
2008-2011
Czech Academy of Sciences, Institute of Microbiology
2005-2010
In methanogenic Archaea, the final step of methanogenesis generates methane and a heterodisulfide coenzyme M B (CoM-S-S-CoB). Reduction this by reductase to regenerate HS-CoM HS-CoB is an exergonic process. Thauer et al. [Thauer, 2008 Nat Rev Microbiol 6:579–591] recently suggested that in hydrogenotrophic methanogens energy reduction powers most endergonic reaction pathway, catalyzed formylmethanofuran dehydrogenase, via flavin-based electron bifurcation. Here we present evidence these two...
In this work we assessed the potential for denitrification linked to methanol consumption in a microbial community inhabiting top layer of sediment pristine lake, Lake Washington Seattle. Stable isotope probing with (13) C was implemented near situ conditions and also presence added nitrate. This revealed that bacterial population involved uptake dominated by species belonging Methylophilaceae, most prominently genus Methylotenera. Based on relative abundance specific phylotypes DNA clone...
Hydrogel-based wearable epidermal sensors (HWESs) have attracted widespread attention in health monitoring, especially considering their colorimetric readout capability. However, it remains challenging for HWESs to work at extreme temperatures with long term stability due the existence of water. Herein, a transparent sensor thermal compatibility and smart multi-signals monitoring is developed, based on an anti-freezing anti-drying hydrogel high transparency (over 90% transmittance),...
Abstract Background Porphyromonas gingivalis is a periodontal pathogen that resides in complex multispecies microbial biofilm community known as dental plaque. Confocal laser scanning microscopy showed P. can assemble into communities vitro with Streptococcus gordonii and Fusobacterium nucleatum , common constituents of Whole cell quantitative proteomics, along mutant construction analysis, were conducted to investigate how adapts this three species community. Results 1156 proteins detected...
Spectral counting, a promising method for quantifying relative changes in protein abundance mass spectrometry-based proteomic analysis, was compared to metabolic stable isotope labeling using 15N/14N "heavy/light" peptide pairs. The data were drawn primarily from Methanococcus maripaludis experiment comparing wild-type strain with mutant deficient key enzyme relevant energy metabolism. dataset contained both proteome and transcriptome measurements. normalization technique used previously the...
The ability of some microbial species to oxidize monomethylamine via glutamate-mediated pathways was proposed in the 1960s; however, genetic determinants have never been described. In present study we describe a gene cluster essential for operation N-methylglutamate pathway methylotrophic beta-proteobacterium Methyloversatilis universalis FAM5. Four major polypeptides from protein fractions displaying high activities synthetase, dehydrogenase and gamma-glutamylmethylamide synthetase were...
This study was conducted to examine the effect of purple corn anthocyanin on performance, meat quality, muscle antioxidant activity, gene expression, and fatty acid profiles in goats. The feeding trial period lasted 74 d. adaptation 14 d, formal experimental 60 Eighteen Qianbei-pockmarked goats (Guizhou native goat breed; body weight, 21.38 ± 1.61 kg; mean standard deviation) were randomly allotted into three equal groups, including a control with no pigment (PCP) groups receiving either 0.5...
For the archaeon Methanococcus maripaludis, a fully sequenced and annotated model species of hydrogenotrophic methanogen, we report validation quantitative protein level expression ratios on proteome-wide basis. Using an approach based multidimensional capillary HPLC quadrupole ion trap mass spectrometry, coverage gene approached that currently achievable with transcription microarrays. Comprehensive spectrometry-based proteomics spotted cDNA arrays were used to compare global mRNA levels in...
Abstract Whole‐cell quantitative proteomic analyses were conducted to investigate the change from an extracellular intracellular lifestyle for Porphyromonas gingivalis , a Gram‐negative pathogen associated with periodontal disease. Global protein abundance data P. strain ATCC 33277 internalized 18 h within human gingival epithelial cells and controls exposed cell culture medium obtained at sufficient coverage provide strong evidence that these changes are profound. A total of 385 proteins...
Abstract Background Methanogenic Archaea play key metabolic roles in anaerobic ecosystems, where they use H 2 and other substrates to produce methane. Methanococcus maripaludis is a model for studies of the global response nutrient limitations. Results We used high-coverage quantitative proteomics determine M. growth-limiting levels , nitrogen, phosphate. Six ten percent proteome changed significantly with each limitation. limitation increased abundance wide variety proteins involved...
In order to validate a gel free quantitative proteomics assay for the model methylotrophic bacterium Methylobacterium extorquens AM1, we examined M. AM1 proteome under single carbon (methanol) and multicarbon (succinate) growth, conditions that have been studied decades which extensive corroborative data compiled. total, 4447 proteins from database containing 7556 putative ORFs could be identified with two or more peptide sequences, corresponding qualitative coverage of 58%. Statistically...
Abstract Background Streptococcus gordonii is one of several species that can initiate the formation oral biofilms develop into complex multispecies microbial communities referred to as dental plaque. It in context plaque periodontal pathogens such Porphyromonas gingivalis cause disease. We have previously reported a whole cell quantitative proteomics investigation P. model community S. , and Fusobacterium nucleatum . Here we report adaptation same model. Results 1122 proteins were detected...
Fusobacterium nucleatum is a common oral organism that can provide adhesive and metabolic support to developing periodontal bacterial communities. It within the context of these communities disease occurs. We have previously reported whole cell proteomics analyses Porphyromonas gingivalis Streptococcus gordonii in early-stage with each other F. nucleatum, modeled using 18 h pellets. Here, we report adaptation same experimental conditions as measured by differential protein expression. About...
Methanococcus maripaludis is a mesophilic archaeon that reduces CO2 to methane with H2 or formate as an energy source. It contains two membrane-bound energy-conserving hydrogenases, Eha and Ehb. To determine the role of Ehb, deletion in ehb operon was constructed yield mutant, strain S40. Growth S40 severely impaired minimal medium. Both acetate yeast extract were necessary restore growth nearly wild-type levels, suggesting Ehb involved multiple steps carbon assimilation. However, no...
Abstract The human oral pathogen Porphyromonas gingivalis colonizes the gingival crevice and invades epithelial cells. Multidimensional capillary high‐performance liquid chromatography coupled with tandem mass spectrometry two‐dimensional gel electrophoresis were used to analyze proteome of P. as it adapts a set experimental conditions designed reflect important features an cell environment. 1014 proteins (46% total theoretical proteome) identified in four independent analyses; 479 these...
Methylotenera species, unlike their close relatives in the genera Methylophilus, Methylobacillus, and Methylovorus, neither exhibit activity of methanol dehydrogenase nor possess mxaFI genes encoding this enzyme, yet they are able to grow on methanol. In work, we integrated a genome-wide proteomics approach, shotgun proteomics, transcriptomics transcriptome sequencing (RNA-seq), mobilis JLW8 identify enzymes potentially involved oxidation, with special attention alternative nitrogen sources,...
In recent years, techniques have been developed and perfected for high-throughput identification of proteins their accurate partial sequencing by shotgun nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS), making it feasible to assess global protein expression profiles in organisms with sequenced genomes. We implemented comprehensive proteomics the expressed portion genome Methylobacillus flagellatus during methylotrophic growth. detected a total 1,671 (64% inferred...
Abstract Background Porphyromonas gingivalis is a Gram-negative intracellular pathogen associated with periodontal disease. We have previously reported on whole-cell quantitative proteomic analyses to investigate the differential expression of virulence factors as organism transitions from an extracellular lifestyle. The original results invasive strain P. ATCC 33277 were obtained using genome sequence available at time, W83 [GenBank: AE015924]. present here re-processed dataset recently...