A5002781286- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Zoonotic diseases and public health
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- Malaria Research and Control
- Infectious Encephalopathies and Encephalitis
- COVID-19 Clinical Research Studies
- Insect and Pesticide Research
- Infective Endocarditis Diagnosis and Management
- COVID-19 diagnosis using AI
- Bacillus and Francisella bacterial research
- Brucella: diagnosis, epidemiology, treatment
- Influenza Virus Research Studies
- Electrolyte and hormonal disorders
- Complement system in diseases
- Restraint-Related Deaths
- Actinomycetales infections and treatment
- HIV/AIDS drug development and treatment
- Muscle and Compartmental Disorders
- Cannabis and Cannabinoid Research
- Infectious Disease Case Reports and Treatments
- Animal Disease Management and Epidemiology
- Hepatitis B Virus Studies
University of Osijek
2018-2025
Klinički bolnički centar Osijek
2019-2025
Onderstepoort Veterinary Institute
2022
University Hospital Centre Zagreb
2018-2019
Tick-borne encephalitis (TBE) represents an important public health problem in Europe. We analyzed the epidemiology of TBE based on data from humans, animals, and Ixodes ricinus ticks endemic regions continental Croatia. In period 2017 to 2023, cerebrospinal fluid (CSF) serum samples 684 patients with neuroinvasive diseases, 2240 horse samples, 300 sheep were tested for TBEV. addition, 8751 I. collected. CSF using RT-PCR. Serological tests (serum, CSF) performed commercial ELISA,...
In 2018, Croatia reported the largest outbreak of West Nile virus (WNV) infections as well re-occurrence human Usutu (USUV) infections. For first time, fatal WNV and USUV were detected in wild birds. We analysed epidemiological characteristics molecular epidemiology during 2018 transmission season. From April to November, 178 patients with neuroinvasive disease 68 febrile tested for USUV. Viral RNA was cerebrospinal fluid (CSF) urine samples using a real-time RT-PCR. Positive by nested...
Neuroinvasive flaviviruses such as tick-borne encephalitis virus (TBEV) and West Nile (WNV) are widely distributed in continental Croatian regions. We analyzed clinical characteristics, laboratory parameters, molecular epidemiology of neuroinvasive flavivirus infections eastern Croatia. A total 43 patients with confirmed infection hospitalized from 2017 to 2023 were included the study. Reverse-transcription polymerase chain reaction (RT-qPCR) was used detect RNA samples (cerebrospinal fluid;...
Data on the immune response to West Nile virus (WNV) are limited. We analyzed antiviral cytokine in serum and cerebrospinal fluid (CSF) samples of patients with WNV fever neuroinvasive disease using a multiplex bead-based assay for simultaneous quantification 13 human cytokines. The panel included cytokines associated innate early pro-inflammatory responses (TNF-α/IL-6), Th1 (IL-2/IFN-γ), Th2 (IL-4/IL-5/IL-9/IL-13), Th17 (IL-17A/IL-17F/IL-21/IL-22) key anti-inflammatory IL-10. Elevated...
Flaviviruses are a heterogeneous group of viruses that may induce broad antigenic cross-reactivity. We present patient who was admitted to the infectious disease department with symptoms suggestive aseptic meningitis. During clinical workup, reported tick bite two weeks before onset. High titers IgM and IgG antibodies tick-borne encephalitis virus (TBEV) were found in both serum cerebrospinal fluid (CSF) samples, indicating acute TBEV infection. West Nile (WNV) Usutu (USUV) and/or also...
Tick-borne encephalitis virus (TBEV) is one of the most significant arboviruses affecting human central nervous system (CNS) in Europe. Data on cytokine response TBEV infection are limited.We analyzed serum, cerebrospinal fluid (CSF) and urine samples patients with TBE. The control group consisted 'febrile headache' who had normal CSF cytology. panel included 12 cytokines: TNF-α, IL-6, Th1 (IL-2, IFN-γ), Th2 (IL-4, IL-5, IL-13), Th9 (IL-9), Th17 (IL-17A, IL-17F), Th22 (IL-22) cytokines...
Growth differentiation factor 15 (GDF-15) is a stress-induced cytokine associated with acute and chronic inflammatory states. This prospective observational study aimed to investigate the prognostic roles of GDF-15 routine clinical laboratory parameters in COVID-19 patients. Upon admission 95 adult hospitalized patients Croatia, blood analysis was performed, medical data were collected. The categorized based on survival, ICU admission, hospitalization duration. Logistic regression ROC curve...
Background: Gram-positive opportunistic bacteria of the Nocardia species are responsible for a large spectrum infections, such as pneumonia, skin and more widespread conditions, including brain abscesses. Case Description: A 67-year-old male patient suffered from headache, gait disorder, vertigo week before admission to our department. An enhanced magnetic resonance imaging scan revealed mediosagittal hyperintense infratentorial lesion with concomitant compression fourth ventricle. The...
(Re-)emerging arboviruses, such as tick-borne encephalitis virus (TBEV), West Nile (WNV), and Usutu (USUV), are continuously increasing in incidence. We analyzed the epidemiological characteristics of flavivirus infections humans, sentinel animals, mosquitoes detected 2022 transmission season Croatia. From April to November 2022, 110 hospitalized patients with neuroinvasive diseases (NID) were tested for presence arboviruses. RT-qPCR was used detect TBEV, WNV, USUV RNA. An ELISA...
The brucellosis and Q-fever coinfection is very rarely reported. To our knowledge, this the first case report of concomitant Q-fever, most likely imported in Croatia. A 30-year-old male agricultural worker was hospitalized on 22 April 2017 after a ten days fever up to 40°C with chills, shivering, excessive sweating, general weakness, loss appetite headache. month half prior hospitalization he lost 18 kg body weight. Three weeks before patient returned from Kupres (Bosnia Herzegovina) where...
West Nile virus (WNV) immunoglobulin M (IgM) antibodies have been shown to persist for up 500 days in certain patients. To evaluate the usefulness of G (IgG) avidity assessment diagnosis WNV infection, we analyzed 54 IgM- and/or IgG-positive serum samples from 39 patients with neuroinvasive disease and 15 asymptomatic cases tested during a seroprevalence investigation.Serological tests (WNV IgM/IgG antibody detection, IgG avidity) were performed using commercially available enzyme-linked...
Tahyna orthobunyavirus (TAHV) is widely distributed in continental Europe. Very few studies have analyzed TAHV seroprevalence Croatia. We the prevalence of RNA and antibodies Croatian patients with neuroinvasive disease (NID).A total 218 unsolved NID detected during five consecutive arbovirus transmission seasons (April 2017-October 2021) were tested. Cerebrospinal fluid (CSF) urine samples tested for using RT-PCR. In addition, CSF serum a virus neutralization test (VNT).Clinical...
Although the Bhanja bandavirus (BHAV) is widely distributed in some European countries, human infections are rarely reported. This study analyzed prevalence of BHAV antibodies patients with neuroinvasive diseases unsolved etiology. A total 254 Croatian who developed neurological symptoms during four consecutive arbovirus transmission seasons (April 2017-October 2021) were tested. Cerebrospinal fluid (CSF) and urine samples tested using RT-qPCR. In addition, CSF serum a virus neutralization...
Background: One of the major challenges in improving sepsis care is early prediction complications. The endocannabinoid system has been intensely studied recent years; however, little known about its role humans. This study aimed to assess prognostic endocannabinoids, anandamide (AEA) and 2-arachidonoylglycerol (2-AG), as predictors mortality, invasive mechanical ventilation (IMV) requirement, length stay (LOS) patients with sepsis. Materials Methods: In total, 106 confirmed were enrolled...
Streptococcus constellatus (SC) is a species of belonging to the anginosus group, along with and intermedius. Despite its commensal nature, underlying risk factors medical conditions might lead various anatomic site infections caused by this opportunistic pathogen. Although SC have mostly been associated bacteremia, some case reports abscess empyema formation documented. Herein, we report middle-aged female patient who initially presented radiculopathy symptoms. Subsequent neurologic imaging...
The most important use of serology in the COVID-19 diagnostics is for determination extent disease population . However, immunoassays could represent an additional diagnostic method, especially patients with exposure history and clinical symptoms compatible who failed to be confirmed by RT-PCR. We analyzed preliminary results six tests diagnosis SARS-CoV-2. Three point-of-care lateral flow chromatographic (POC): ACRO, AMP ENCODE three enzyme (ELISA): DiaPro, Vircell Euroimmun were used. A...
Tick-borne encephalitis virus (TBEV) and West Nile (WNV) are the most important neuroinvasive arboviruses detected in Europe. In this study, we analyzed cerebrospinal fluid (CSF) concentrations of 12 proinflammatory chemokines (CCL2, CCL3, CCL4, CCL11, CCL17, CCL20, CXCL1, CXCL5, CXCL8, CXCL9, CXCL10, CXCL11) 77 patients with diseases (NIDs). Flavivirus infection was confirmed 62 (TBEV WNV 31 each), while 15 etiology NID not determined (NDE). Similar patterns high-level expression regulating...
BACKGROUND The diagnosis of West Nile virus (WNV) is challenging due to short-term and low-level viremia, flavivirus cross-reactivity, long immunoglobulin M (IgM) persistence. AIM To evaluate different methods for WNV detection [reverse transcription-polymerase chain reaction (RT-PCR), IgM/IgG antibodies, IgG avidity] in serum, cerebrospinal fluid (CSF), urine samples patients with confirmed infection. METHODS study included neuroinvasive infection (n = 62), asymptomatic seropositive...
Purpose: West Nile virus (WNV) IgM antibodies have been shown to persist for up 500 days or even longer in some patients. Therefore, additional tests are needed confirm primary infection. The aim of this study was analyze the value IgG avidity diagnosis symptomatic and asymptomatic WNV Methods & Materials: A total 54 and/or positive serum samples collected from 39 patients with neuroinvasive disease (meningitis/encephalitis/myelitis) 15 persons were tested avidity. Serological performed...