A5061438426- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Cancer Cells and Metastasis
- Proteins in Food Systems
- Diet, Metabolism, and Disease
- Cell death mechanisms and regulation
- Endoplasmic Reticulum Stress and Disease
- Fatty Acid Research and Health
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Membrane Separation and Gas Transport
- Fractal and DNA sequence analysis
- Hepatitis C virus research
- Diabetes and associated disorders
- Protein Hydrolysis and Bioactive Peptides
- Inflammatory mediators and NSAID effects
- Membrane Separation Technologies
- Polysaccharides Composition and Applications
- Food composition and properties
- Phytase and its Applications
- Hepatitis Viruses Studies and Epidemiology
- Analytical Chemistry and Chromatography
- Membrane-based Ion Separation Techniques
- Electrocatalysts for Energy Conversion
- Hepatitis B Virus Studies
Nanjing Medical University
2024
Jiangsu Province Hospital
2024
Jiangsu Cancer Hospital
2024
State Key Laboratory of Food Science and Technology
2023
Jiangnan University
2023
Beijing University of Chemical Technology
2015
University of Southern California
2013
First Affiliated Hospital of Harbin Medical University
2012
Harbin Medical University
2012
Shanghai First People's Hospital
2009-2011
Successful islet transplantation depends on the infusion of sufficiently large quantities islets, which only ∼30% become stably engrafted. Rapid and adequate revascularization transplanted islets is important for survival function. Delayed insufficient can deprive oxygen nutrients, resulting in cell death early graft failure. To improve revascularization, we delivered human vascular endothelial growth factor (VEGF) cDNA to murine followed by under renal capsule diabetic mice. Diabetic...
Dietary advanced glycosylation end products (AGEs) have been linked to insulin resistance in db/db(++) mice. To test whether dietary AGEs play a role the progression of normal mice fed high-fat diets, C57/BL6 were randomly assigned diets (35% g fat), either high (HAGE-HF group; 995.4 units/mg AGE) or low (by 2.4-fold LAGE-HF 329.6 AGE content for 6 months. Age-matched and regular diet (5% fat, 117.4 served as controls. After months, 75% HAGE-HF diabetic exhibited higher body weight (P...
Mitochondria-mediated production of reactive oxygen species (ROS) plays a key role in apoptosis. Mitochondrial phospholipid cardiolipin molecules are likely the main target ROS because they particularly rich polyunsaturated fatty acids. They also located inner mitochondrial membrane near ROS-producing sites. Under physiological conditions mitochondria can repair peroxidative damage part through remodeling mechanism via deacylation-reacylation cycle mediated by phospholipase A2 (PLA2) and...
Mitochondrial production of reactive oxygen species and oxidation cardiolipin are key events in initiating apoptosis. We reported that group VIA Ca2+-independent phospholipase A2 (iPLA2β) localizes protects β-cell mitochondria from oxidative damage during staurosporine-induced Here, we used iPLA2β-null (iPLA2β−/−) mice to investigate the role iPLA2β repair mitochondrial membranes. show islets isolated iPLA2β−/− more sensitive apoptosis than those wild-type littermates 2 wk daily ip...
Glioblastoma, the most lethal primary brain tumor, harbors glioma stem cells (GSCs) that not only initiate and maintain malignant phenotypes but also enhance therapeutic resistance. Although frequently mutated in glioblastomas, function regulation of PTEN PTEN-intact GSCs are unknown. Here, we found directly interacted with MMS19 competitively disrupted MMS19-based cytosolic iron-sulfur (Fe-S) cluster assembly (CIA) machinery differentiated cells. was specifically succinated at cysteine (C)...
The G1 phase of the cell cycle is characterized by a high rate membrane phospholipid turnover. Cells regulate this turnover coordinating opposing actions CTP:phosphocholine cytidylyltransferase and group VI Ca2+-independent phospholipase A2 (iPLA2). However, little known about how such affects cell-cycle progression. Here, we show that G1-phase essential for proliferation. Specific inhibition iPLA2 arrested cells in cycle. This arrest was associated with marked upregulation tumour suppressor...
Islet Ca2+-independent phospholipase A2 (iPLA2) is postulated to mediate insulin secretion by releasing arachidonic acid in response secretagogues. However, the significance of iPLA2 signaling vivo remains unexplored. Here we investigated physiological role beta-cell lines, isolated islets, and mice. We showed that small interfering RNA-specific silencing expression INS-1 cells significantly reduced insulin-secretory responses glucose. Immunohistochemical analysis revealed mouse islet...
Abstract Due to its low water solubility and slow dissolution rate, genipin was micronized by an antisolvent precipitation process using ethanol as solvent n ‐hexane antisolvent. The effects of various experimental parameters on the mean particle size (MPS) were investigated. By analysis variance, only concentration solution has a significant effect MPS in micronization. Under optimum conditions, with 1.8 μm obtained. characterized methods, e.g., scanning electron microscopy...
Abstract BACKGROUND Polyimide ( PI ) solvent‐resistant nanofiltration SRNF membranes were prepared by a two‐step method. The polyamic acid PAA precursor was synthesized first, and then obtained via non‐solvent induced phase separation Finally, the transformed into thermal imidization effect of molecular weight on structure performance investigated in detail. In addition, influence other processing parameters, such as evaporation time immersion coagulation bath, from with different weights...
Summary. We determined the nucleotide and deduced amino acid sequence of 5′ terminus hepatitis G virus (HGV) genome from isolates varied geographical origins. Our analysis showed that putative non‐coding region (NCR) contains several blocks highly conserved sequences may be useful for development a reverse transcriptase—polymerase chain reaction (RT‐PCR) assay detection HGV RNA. Overall, degree conservation within 669‐nucleotide (nt) terminal was found to range 99.5% 86% identity. also NCR...
In recent years, the theory of cancer stem cells has provided a new perspective on treatment cancers including breast cancer. The accurate sorting is critical. procedure consist 4 steps: isolation side population, serum-free suspension culture,determination specific cell surface markers and activity aldehyde dehydrogenase 1 ( ALDH1 ) enzymatic through ALDEFLUOR assay. Some studies choose drug resistance as an additional method for because enrichment after chemotherapy . controversy about...
1126 Background: The purpose of this study was to investigate the relationship between pAkt and CD44v6 expression breast cancer after surgery. We also evaluated patterns predictive value pAKT in tissues. Methods: Expression protein detected using immunohistochemistry 98 patients. correlation pAkt, CD44v6, other disease-related characteristics with treatment outcome investigated. Kaplan-Meier survival analysis Cox regression were used observe variables prognostic value. Results: Of all these...
Calcium-independent phospholipase A2β (iPLA2β) can catalyze hydrolysis of the sn-2 fatty acyl bond phospholipids to release free unsaturated acids and lysophospholipids, plays an im-portant role in life activities. Features iPLA2β amino acid sequence include ankyrin-repeats, a serine li-pase consensus calmodulin-binding domain ,etc. The activity iPLA213 is regulated by calmod-ulin, protein kinase C (PKC), ATP some other factors. Studies have found that expression iPLA2βin islet β-cells high,...