A5101874640- Congenital Heart Disease Studies
- Congenital heart defects research
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Renal and Vascular Pathologies
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- Diabetes and associated disorders
- Genomic variations and chromosomal abnormalities
- Diet, Metabolism, and Disease
- Diabetes Management and Research
- Advanced Glycation End Products research
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- Neurological diseases and metabolism
- Phosphodiesterase function and regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Respiratory viral infections research
- Turtle Biology and Conservation
- Malaria Research and Control
- Gene expression and cancer classification
- Endoplasmic Reticulum Stress and Disease
- Animal Genetics and Reproduction
- Biosensors and Analytical Detection
Children's Hospital of Fudan University
2018-2024
Texas A&M University – Commerce
2018-2021
Stanford University
2016-2017
Diabetes Australia
2014
Icahn School of Medicine at Mount Sinai
2009-2011
Yonsei University
2004-2009
Université Laval
2006-2008
Wilfrid Laurier University
2008
University of Hawaiʻi at Mānoa
2003-2007
Significance Suppression of NAD + -dependent sirtuin 1 (SIRT1) is linked to dementia or Alzheimer’s disease (AD) and the metabolic syndrome (MS). Because advanced glycation end products (AGEs) promote MS neurotoxicity, we conducted studies C57BL6 mice fed isocaloric diets containing defined AGEs [methyl-glyoxal derivatives (MG)] determine whether food AD MS. MG -fed, but not − developed brain SIRT1 deficiency, amyloid-β deposits, cognitive motor deficits, These findings were validated in...
Advanced glycation end products (AGEs) are implicated in diabetic complications. However, their role β-cell dysfunction is less clear. In this study we examined the effects of AGEs on islet function mice and isolated islets. AGE-BSA or BSA was administered ip to normal twice a day for 2 wk. We showed that AGE-BSA-treated exhibited significantly higher glucose levels lower insulin response challenge than did BSA-treated mice, although there were no significant differences sensitivity...
Pharmacologic expansion of endogenous β cells is a promising therapeutic strategy for diabetes. To elucidate the molecular pathways that control β-cell growth we screened ∼2400 bioactive compounds rat replication-modulating activity. Numerous hit impaired or promoted replication, including CC-401, an advanced clinical candidate previously characterized as c-Jun N-terminal kinase inhibitor. Surprisingly, CC-401 induced rodent (in vitro and in vivo) human vitro) replication via...
MCD (malonyl-CoA decarboxylase), which catalyses decarboxylation of malonyl-CoA, is known to play an important role in the regulation malonyl-CoA concentration. Recently, it has been observed that expression significantly decreased hearts PPARα (peroxisome-proliferator-activated receptor α) (−/−) mice, where rate fatty-acid oxidation by increased level [Campbell, Kozak, Wagner, Altarejos, Dyck, Belke, Severson, Kelly and Lopaschuk (2002) J. Biol. Chem. 277, 4098–4103]. This suggests may be...
ABSTRACT Proinflammatory cytokines secreted by memory CD8 + and CD4 T cells are thought to play a direct role in the pathogenesis of dengue virus infection increasing vascular permeability thereby inducing pathophysiologic events associated with hemorrhagic fever shock syndrome. Severe disease is frequently observed setting secondary heterologous serotypes, suggesting for cross-reactive immunopathogenesis severe disease. We used large panel well-characterized virus-specific T-cell clones...
Mitochondrial production of reactive oxygen species and oxidation cardiolipin are key events in initiating apoptosis. We reported that group VIA Ca2+-independent phospholipase A2 (iPLA2β) localizes protects β-cell mitochondria from oxidative damage during staurosporine-induced Here, we used iPLA2β-null (iPLA2β−/−) mice to investigate the role iPLA2β repair mitochondrial membranes. show islets isolated iPLA2β−/− more sensitive apoptosis than those wild-type littermates 2 wk daily ip...
Loss of insulin-producing β-cell mass is a hallmark type 2 diabetes in humans and diabetic db/db mice. Pancreatic β-cells can modulate their response to variety physiological pathophysiological cues. There are currently few effective therapeutic approaches targeting regeneration although some anti-diabetic drugs may positively affect mass. Here we show that oral administration FTY720, sphingosine 1-phosphate (S1P) receptor modulator, mice normalizes fasting blood glucose by increasing...
Infantile neuroaxonal dystrophy (INAD) is a progressive, autosomal recessive neurodegenerative disease characterized by axonal dystrophy, abnormal iron deposition and cerebellar atrophy. This was recently mapped to PLA2G6, which encodes group VI Ca2+-independent phospholipase A2 (iPLA2 or iPLA2β). Here we show that genetic ablation of PLA2G6 in mice (iPLA2β-/-) leads the development atrophy age 13 months. Atrophied cerebella exhibited significant loss Purkinje cells, as well reactive...
Fabrication of microarray devices using traditional glass slides is not easily adaptable to integration into microfluidic systems. There thus a need for the development polymeric materials showing high hybridization signal-to-background ratio, enabling sensitive detection microbial pathogens. We have developed such plastic supports suitable highly DNA hybridizations. The proof concept this technology was done through four human respiratory viruses that were amplified and labeled with...
Islet β-cells adapt to insulin resistance through increased secretion and expansion. Type 2 diabetes typically occurs when prolonged exceeds the adaptive capacity of β-cells. Our prior screening efforts led discovery that adenosine kinase (ADK) inhibitors stimulate β-cell replication. Here, we evaluated whether ADK disruption in mouse affects mass and/or protects against high-fat diet (HFD)-induced glucose dysregulation. Mice targeted at Adk locus were bred Rip-Cre Ins1-Cre/ERT1Lphi mice...
Abstract Background Previous studies demonstrating the efficacy of insulin gene therapy have mostly involved use adenoviral vectors or naked DNA to deliver gene. However, this procedure may not guarantee long‐term production. To improve performance, we prepared recombinant adeno‐associated viral (rAAV) harboring encoding a furin‐modified human under cytomegalovirus (CMV) promoter [rAAV‐hPPI(F12)]. Methods Streptozotocin (STZ)‐induced diabetic Sprague‐Dawley rats were used as animal model....
Abstract Background Syndromic congenital heart disease (CHD) is among the most severe conditions in pediatric population. Copy number variant (CNV) an important cause of syndromic CHD, but few studies focused on CNVs related to these patients China. The present study aimed identify pathogenic associated with CHD Chinese Methods A total 109 sporadic were applied chromosomal microarray analysis (CMA). Phenotype spectrum or likely was analyzed. CHD-related genes prioritized from within by...
Summary Congenital heart defect (CHD) is one of the most common cardiovascular diseases, affecting approximately 0.8% live births. The transcription factor GATA4 has been known to play a key role in cardiac development. In this study, we performed whole exome sequencing nine unrelated CHD patients and found two rare deleterious missense variants gene (c.C487T,p.P163S c.C1223A,p.P408Q) (ExAC <0.001 CADD >15) three cases that were confirmed by Sanger sequencing. Subsequently, these...
Loss of insulin-producing β-cells is a central feature diabetes. While variety potential replacement therapies are being explored, expansion endogenous pancreatic islet remains an attractive strategy. have limited spontaneous regenerative activity; consequently, crucial research effort to develop precise understanding the molecular pathways that restrain β-cell growth and identify drugs capable overcoming these restraints. Herein automated high-content image-based primary-cell screening...
Abstract Background: Heterotaxy (HTX) is a thoracoabdominal organ anomaly syndrome and commonly accompanied by congenital heart disease (CHD). The aim of this study was to analyze rare copy number variations (CNVs) in HTX/CHD cohort examine the potential mechanisms contributing HTX/CHD. Methods: Chromosome microarray analysis used identify CNVs 120 unrelated patients, available samples from parents were confirm inheritance pattern. Potential candidate genes region prioritized via DECIPHER...
Aim: The intracellular concentration of malonyl‐CoA, a key regulator fatty acid oxidation, is determined both from its synthesis by acetyl‐CoA carboxylase and degradation malonyl‐CoA decarboxylase (MCD). aim our study was to investigate the activity mRNA expression MCD under insulin resistance after treatment with sensitizers in different tissues. Methods: We treated 18‐week Otusuka Long‐Evans Tokushima Fatty (OLETF) rats pioglitazone (10 mg/kg/day) or metformin (300 for 8 weeks diabetic...
Summary Acrodysostosis is an extremely rare disorder at birth, that is, characterized by skeletal dysplasia with short stature and midfacial hypoplasia, which has been reported to be caused PDE4D PRKAR1A gene mutations. Here, a Chinese boy acrodysostosis, ventricular septal defect, pulmonary hypertension was recruited for our study, his clinical biochemical characteristics were analyzed. A novel de novo heterozygous missense mutation (NM_001104631: c.2030A>C, p.Tyr677Ser) of the detected...
The present study explores an RNA we have discovered in human heart that induces differentiation of mouse embryonic stem cells and induced pluripotent into cardiomyocytes vitro. We designated this as Cardiac Inducing or CIR. now find CIR also fibroblasts (MEF) to form For these studies, human-derived is transfected MEF using lipofectamine. CIR-transfected exhibit spindle-shaped cells, characteristic myocardial culture express cardiac-specific troponin-T cardiac tropomyosin. As such, the...
Background: As a key component in the NOTCH signaling pathway, HES1 plays an important role vertebrate heart development. Variants coding sequence are known to be associated with congenital disease (CHD). However, little is about non-coding variants and their association risk of developing CHD. Method Results: We initially analyzed gene 12 unrelated CHD families by direct sequencing identified previously unreported promoter region variant (NM_005524.4: c.−1279−1278 insAC, rs148941464) four...