A5025449020- Glioma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Advanced Neuroimaging Techniques and Applications
- RNA Research and Splicing
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Extracellular vesicles in disease
- Intracranial Aneurysms: Treatment and Complications
- ATP Synthase and ATPases Research
- Cancer Research and Treatments
- Advanced MRI Techniques and Applications
- Telomeres, Telomerase, and Senescence
- Heat shock proteins research
- Radiomics and Machine Learning in Medical Imaging
- NF-κB Signaling Pathways
- Vascular Malformations Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Pituitary Gland Disorders and Treatments
Nanjing Medical University
2014-2025
Jiangsu Province Hospital
2014-2025
Jiangsu Cancer Hospital
2020-2024
Nanjing Normal University
2024
Air Force Engineering University
2022
Second Hospital of Shanxi Medical University
2017
Shanxi Medical University
2017
Beijing Tian Tan Hospital
2014
Capital Medical University
2014
Thomas Jefferson University
2014
Abstract Background Accumulating evidence shows that long noncoding RNAs (lncRNAs) are important regulator molecules involved in diverse biological processes. Acquired drug resistance is a major challenge the clinical treatment of glioblastoma (GBM), and lncRNAs have been shown to play role chemotherapy resistance. However, underlying mechanisms by which lncRNA mediates TMZ GBM remain poorly characterized. Methods Quantitative reverse transcription PCR (qRT-PCR) fluorescence situ...
MicroRNAs (miRNAs) are highly evolutionarily-conserved non-coding small RNAs, which were first identified in Caenorhabditis elegans. Let-7 miRNA is involved the regulation of gene expression cells. Several novel factors and feedback loops synthesis let-7 have been additional target genes found. has also shown to be significantly correlated with occurrence development cancer results preliminary studies suggest that it oncogenic pathways numerous types tumors. is, therefore, a potential...
MIR155 host gene (MIR155HG) is a long noncoding RNA that has been considered as the primary micro (mi)RNA of miR-155. MIR155HG plays an essential role in hematopoiesis, inflammation, and tumorigenesis. Our study investigated clinical significance, biological function, mechanisms, small-molecule inhibitors MIR155HG/miR-155 axis glioma. We analyzed expression correlation with glioma grade patient survival using 2 different datasets. Biological significance was elucidated through series vitro...
Glioma is one of the most aggressive and lethal human brain tumors. Accumulating evidence shows that microRNAs play important roles in cancers, including glioma. Previous studies reported miR-124 levels were downregulated glioma specimens. Here, we further investigate potential role The expression detected specimens by quantitative reverse transcriptase PCR. direct targets identified bioinformatics analysis validated immunoblotting luciferase reporter assay. effects on cell proliferation...
Rationale: Glioma stem-like cells (GSCs) contribute to temozolomide (TMZ) resistance in gliomas, although the mechanisms have not been delineated. Methods:In vitro functional experiments (colony formation assay, flow cytometric analysis, TUNEL assay) were used assess ability of extracellular vesicles (EVs) from hypoxic GSCs promote TMZ glioblastoma (GBM) cells. RNA sequencing and quantitative Reverse Transcription-PCR employed identify miRNA EVs. Chromatin immunoprecipitation assays...
Abstract Long noncoding RNAs (lncRNAs) are involved in glioma initiation and progression. Glioma stem cells (GSCs) essential for tumor initiation, maintenance, therapeutic resistance. However, the biological functions underlying mechanisms of lncRNAs GSCs remain poorly understood. Here, we identified that LINC00839 was overexpressed GSCs. A high level associated with GBM progression radiation METTL3-mediated m6A modification on enhanced its expression a YTHDF2-dependent manner....
Radiotherapy is a major component of standard-of-care treatment for gliomas, the most prevalent type brain tumor. However, resistance to radiotherapy remains concern. Identification mechanisms governing radioresistance in gliomas could reveal improved therapeutic strategies treating patients. Here, we report that mitochondrial metabolic pathways are suppressed radioresistant through integrated analyses transcriptomic data from glioma specimens and cell lines. Decreased expression peroxisome...
Temozolomide (TMZ) is a standard treatment for glioblastoma (GBM) patients. However, TMZ has moderate therapeutic effects due to chemoresistance of GBM cells through less clarified mechanisms. Here, we demonstrate that TMZ-derived 5-aminoimidazole-4-carboxamide (AICA) converted AICA ribosyl-5-phosphate (AICAR) in cells. This conversion catalyzed by hypoxanthine phosphoribosyl transferase 1 (HPRT1), which highly expressed human GBMs. As the bona fide activator AMP-activated protein kinase...
Glioblastoma multiforme (GBM) is the most malignant type of glioma. Integrated classification based on mRNA expression microarrays and whole–genome methylation subdivides GBM into five subtypes: Classical, Mesenchymal, Neural, Proneural-CpG island methylator phenotype (G-CIMP) Proneural-non G-CIMP. Biomarkers that can be used to predict prognosis in each subtype have not been systematically investigated. In present study, we Cox regression risk-score analysis construct respective prognostic...
// Dongfeng Han 1, * , Wenjin Wei Xincheng Chen Yaxuan Zhang Yingyi Wang 1 Junxia Xiefeng Tianfu Yu Qi Hu Ning Liu Yongping You Department of Neurosurgery, The First Affiliated Hospital Nanjing Medical University, 210029, China These authors have contributed equally to this work Correspondence to: You, e-mail: YYPL9@njmu.edu.cn Keywords: fenofibrate, PPARα, RelA, PKM2, Warburg effect Received: February 16, 2015 Accepted: June 19, Published: 30, 2015...
Glioblastoma, the most lethal primary brain tumor, harbors glioma stem cells (GSCs) that not only initiate and maintain malignant phenotypes but also enhance therapeutic resistance. Although frequently mutated in glioblastomas, function regulation of PTEN PTEN-intact GSCs are unknown. Here, we found directly interacted with MMS19 competitively disrupted MMS19-based cytosolic iron-sulfur (Fe-S) cluster assembly (CIA) machinery differentiated cells. was specifically succinated at cysteine (C)...
Background: Surgical intervention, complemented by radiotherapy and chemotherapy with temozolomide, constitutes the conventional treatment protocol for patients newly diagnosed grade 4 glioma. We have conducted a research to evaluate efficacy safety of an integrated regimen that incorporates tumor-treating fields concurrent chemoradiotherapy. Methods: This retrospective analyzed clinical data 39 adults who were World Health Organization (WHO) gliomas at First Affiliated Hospital Nanjing...
// Rui Li 1 , Xincheng Chen Yongping You Xiefeng Wang Yanwei Liu 2 Qi Hu Wei Yan Department of Neurosurgery, The First Affiliated Hospital Nanjing Medical University, Nanjing, China Beijing Neurosurgical Institute, Capital Beijing, Correspondence to: Yan, e-mail: neuro_yw@njmu.edu.cn Keywords: recurrent glioblastoma, primary molecular characteristics, clinical features Received: March 25, 2015 Accepted: August Published: September 04, ABSTRACT Glioblastoma multiforme is the most common...
Fenofibrate, a fibric acid derivative, is known to possess lipid-lowering effects. Although fenofibrate-induced peroxisome proliferator-activated receptor alpha (PPARα) transcriptional activity has been reported exhibit anticancer effects, the underlying mechanisms are poorly understood. In this study, we investigated behind antiproliferative effects of fenofibrate in U87MG cells (human glioma cell line) using WST-8 Cell Proliferation Assay Kit. Furthermore, examined genome-wide gene...
Abstract Background Glucose metabolic reprogramming is a significant hallmark of malignant tumors including GBM. Previous studies suggest that microRNAs play key roles in modulating this process GBM cells. miR-181b acts as tumor suppressor miRNA influencing glioma tumorigenesis. Our previous results showed was down-regulated cells and tissues. Methods The extracellular acidification rate (ECAR), colony formation assay levels Glut1 PKM2 were measured to assess the glucose proliferation...
Rapid tissue differentiation at the molecular level is a prerequisite for precise surgical resection, which of special value treatment malignant tumors, such as glioblastoma (GBM). Herein, SERS-active microneedle prepared by modifying glutathione (GSH)-responsive molecules, 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), on surface Au@Ag substrates distinction different GBM tissues. Since Raman signals DTNB@Au@Ag can be collected both portable and benchtop spectrometers, distribution GSH in...
Chromobox homolog 7 (CBX7) cooperates with other polycomb group (PcG) proteins to maintain target genes in a silenced state. However, the precise role of CBX7 tumor progression is still controversial. We found that expression four public databases was significantly lower high grade glioma (HGG). The reduced correlated poor outcome HGG patients. Both KEGG and GO analyses indicated were negatively strongly associated cell cycle pathway. observed decreased protein levels enhanced cells...