A5066020707- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- CAR-T cell therapy research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Advanced Fluorescence Microscopy Techniques
- Bioinformatics and Genomic Networks
- Biosimilars and Bioanalytical Methods
- Cell Image Analysis Techniques
- Cancer, Hypoxia, and Metabolism
- Signaling Pathways in Disease
- Glioma Diagnosis and Treatment
- Intensive Care Unit Cognitive Disorders
- Fungal Biology and Applications
- Chromatography in Natural Products
- RNA Research and Splicing
- Lymphoma Diagnosis and Treatment
- Antimicrobial Peptides and Activities
- Neuroinflammation and Neurodegeneration Mechanisms
- Meat and Animal Product Quality
- Pressure Ulcer Prevention and Management
- Frailty in Older Adults
- Microtubule and mitosis dynamics
- S100 Proteins and Annexins
Nanjing Medical University
2018-2024
Changhai Hospital
2023-2024
Second Military Medical University
2023-2024
Jiangsu Province Hospital
2018-2022
Guangxi University
2022
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2022
Shanghai Cell Therapy Research Institute
2019-2022
Jiangsu Cancer Hospital
2020
East China University of Science and Technology
2018
Cellular Biomedicine Group (United States)
2017
Acquired drug resistance is a constraining factor in clinical treatment of glioblastoma (GBM). However, the mechanisms chemoresponsive tumors acquire therapeutic remain poorly understood. Here, we aim to investigate whether temozolomide (TMZ) GBM was enhanced by long non-coding RNA SBF2 antisense 1 (lncRNA SBF2-AS1) enriched exosomes.LncSBF2-AS1 level TMZ-resistance or TMZ-sensitive tissues and cells were analyzed qRT-PCR FISH assays. A series vitro assay xenograft tumor models performed...
Abstract Background Accumulating evidence shows that long noncoding RNAs (lncRNAs) are important regulator molecules involved in diverse biological processes. Acquired drug resistance is a major challenge the clinical treatment of glioblastoma (GBM), and lncRNAs have been shown to play role chemotherapy resistance. However, underlying mechanisms by which lncRNA mediates TMZ GBM remain poorly characterized. Methods Quantitative reverse transcription PCR (qRT-PCR) fluorescence situ...
Abstract Background Acquired chemoresistance is a major challenge in the clinical treatment of glioblastoma (GBM). Circular RNAs have been verified to play role tumor chemoresistance. However, underlying mechanisms remain unclear. The aim this study was elucidate potential and molecular mechanism circular (circ)RNA ADP-ribosylation factor GTPase activating proteins with Src homology 3 domain, ankyrin repeat Pleckstrin domain 1 (circASAP1) temozolomide (TMZ) resistance GBM. Methods We...
Rationale: Glioma stem-like cells (GSCs) contribute to temozolomide (TMZ) resistance in gliomas, although the mechanisms have not been delineated. Methods:In vitro functional experiments (colony formation assay, flow cytometric analysis, TUNEL assay) were used assess ability of extracellular vesicles (EVs) from hypoxic GSCs promote TMZ glioblastoma (GBM) cells. RNA sequencing and quantitative Reverse Transcription-PCR employed identify miRNA EVs. Chromatin immunoprecipitation assays...
Abstract Background The microglia-mediated inflammatory response is a vital mechanism of secondary damage following traumatic brain injury (TBI), but the underlying microglial activation unclear. Methods Controlled cortical impact (CCI) was induced in adult male C57BL/6J mice, and glutamate used to construct classical vitro model primary microglia. Microglial determined by western blot immunostaining. factors were measured enzyme-linked immunosorbent assay. oxidative stress marker...
The oncogene Aurora kinase A (AURKA) has been implicated in various tumor, yet its role meningioma remains unexplored. Recent studies have suggested a potential link between AURKA and ferroptosis, although the underlying mechanisms are unclear. This study presented evidence of upregulation high grade ability to enhance malignant characteristics. We identified as suppressor erastin-induced ferroptosis meningioma. Mechanistically, directly interacted with phosphorylated kelch-like...
Anti-B-cell maturation antigen (BCMA) chimeric receptor T-cell (CAR T) therapy showed remarkable efficacy in patients with relapsed or refractory multiple myeloma (RRMM). This phase 1 dose-escalation and expansion study developed C-CAR088, a novel second-generation humanized anti-BCMA CAR therapy, assessed the safety of three dosages C-CAR088 RRMM.
Abstract Background Glucose metabolic reprogramming is a significant hallmark of malignant tumors including GBM. Previous studies suggest that microRNAs play key roles in modulating this process GBM cells. miR-181b acts as tumor suppressor miRNA influencing glioma tumorigenesis. Our previous results showed was down-regulated cells and tissues. Methods The extracellular acidification rate (ECAR), colony formation assay levels Glut1 PKM2 were measured to assess the glucose proliferation...
2508 Background: Relapse due to loss of the CD19 targeted epitope presents a therapeutic challenge CAR-T therapy. These patients universally have poor outcome and unmet medical need is high. CD20 proven target for B-NHL, supported by approved widely used monoclonal antibody C-CAR066 novel 2nd generation chimeric antigen receptor T (CAR-T) therapy targeting antigen. Preclinical studies suggest that has superior anti-tumor activity compared CAR-Ts derived from scFVs Leu16, Rituximab...
Background: Mounting evidences have shown the importance of lncRNAs in carcinogenesis and cancer progression. LBX2-AS1 is identified as an oncogenic lncRNA that abnormally expressed gastric lung samples. This study aims to explore potential role regulating proliferation EMT glioma, underlying mechanism. Methods: Relative levels glioma samples cell lines were detected by qRT-PCR FISH. In vivo vitro regulatory effects on examined xenograft model cells. The interaction between SP1 was assessed...
Fat deposition is a crucial element in pig production that affects efficiency, quality and consumer choices. In this study, Duroc pigs, Western, famous lean breed, Luchuan Chinese, native obese were used as animal materials. Transcriptome sequencing was to compare the back adipose tissue of explore key genes regulating fat deposition. The results showed 418 highly expressed pig, 441 pig. addition, function enrichment analysis disclosed DEGs had been primarily enriched lipid metabolism,...
Objectives: Lung cancer is one of the most common cancers worldwide and it leading cause cancer-related mortality. Despite treatment patients with non-small cell lung carcinoma (NSCLC) have improved, molecular mechanisms NSCLC are still to be further explored. Materials Methods: Microarray datasets from Gene Expression Omnibus (GEO) database were selected identify candidate genes associated tumorigenesis progression carcinoma. The differentially expressed (DEGs) identified by GEO2R....
Abstract Increasing evidence has demonstrated that cancer cell metabolism is a critical factor in tumor development and progression; however, its role glioblastoma (GBM) remains limited. In the present study, we classified GBM into three subtypes (MC1, MC2, MC3) through cluster analysis of 153 samples from RNA-sequencing data The Cancer Genome Atlas (TCGA) based on 2752 metabolism-related genes (MRGs). We further explored prognostic value, metabolic signatures, immune infiltration,...
Abstract Background: Glioblastoma multiforme (GBM) is a most common aggressive malignant brain tumor. In recent years, targeted therapy has been increasingly applied in GBM treatment. Methods: the present study, GSE22866 was downloaded from gene expression omnibus (GEO). The genomic and clinical data were obtained TCGA. differentially expressed genes (DEGs) identified functional analysis performed using clusterprofiler. Then, co-expression network for DEGs established “WGCNA” package. Next,...
ABSTRACT Aims and Objectives To explore the relationship between Geriatric Nutritional Risk Index (GNRI) occurrence of Pressure injury (PI) in elderly Intensive Care Unit (ICU) patients. Background PI represent a significant health concern within ICU, where such injuries is notably high among critically ill However, few studies have explored GNRI PI. Design A longitudinal, single‐centre, retrospective study. Methods The study utilised calculation to identify occurrences. comprehensive set...
e14548 Background: Chimeric Antigen Receptor T cells (CAR-Ts) targeting CD19 have shown very promising clinical outcomes in treatment of B-cell linage hematological malignancies. However, many patients with relapsed diseases were found to down-regulated/loss surface expression after CAR-T therapy. To solve this issue single-targeting escape, we explored the application another antigen, CD20, for targeted Methods: We constructed four CD20 CARs (all 4-1BB co-stimulatory signaling) base on...
Abstract Ganoderic acid S, ganoderic T and ganoderal B are the main bioactive triterpenes of Ganoderma lucidum . In this study, mycelia G. were obtained by two‐stage fermentation then extracted ethanol petroleum ether sequentially to obtain crude triterpenes. The sample was further purified recycling high‐speed counter‐current chromatography with n‐ hexane–ethyl acetate–methanol–water (7:12:11:5, v /v/ /v) as optimized two‐phase solvent system. A 16.4 mg aliquot ganoderol a purity 90.4%...
<h3>Background</h3> Aberrant expressions of miRNAs play a critical role in the inflammatory and immune response rheumatoid arthritis (RA). The microRNA cluster which encodes for miR-15a miR-16, located at 13q14.3. Both these microRNAs have been implicated pathogenesis many diseases, but little is known their RA. <h3>Objectives</h3> In this study, we aim to investigate underlying molecular mechanisms MiR-15a/16 RA, will provide new insight into understanding RA identifying novel therapeutics...
Introduction: Interim response assessment is performed to identify patients whose disease has not responded or progressed on induction therapy. The Lugano Response Criteria been widely used for DLBCL. Patients with interim reduction percentage of the sum products greatest perpendicular diameters multiple lesions (iΔSPD) between baseline and contrast-enhanced computed tomographic (CECT) less than 50% are regarded as non-sensitive therapy may benefit from a change. However, there no consensus...