A5014932260- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Pituitary Gland Disorders and Treatments
- Glioma Diagnosis and Treatment
- Adrenal and Paraganglionic Tumors
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cardiac Fibrosis and Remodeling
- Global Educational Reforms and Inequalities
- Functional Brain Connectivity Studies
- Advanced Neuroimaging Techniques and Applications
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Histone Deacetylase Inhibitors Research
- Blood groups and transfusion
- Growth Hormone and Insulin-like Growth Factors
- Cancer, Lipids, and Metabolism
- Meningioma and schwannoma management
- Inflammatory Biomarkers in Disease Prognosis
- Insect and Arachnid Ecology and Behavior
- Genetics, Aging, and Longevity in Model Organisms
- Signaling Pathways in Disease
The University of Texas MD Anderson Cancer Center
2020-2025
Tumor Hospital of Guangxi Medical University
2022
Blind and Sight Impaired Society
2022
Nanjing Medical University
2015-2021
The University of Texas Health Science Center at Houston
2021
Sun Yat-sen Memorial Hospital
2021
Sun Yat-sen University
2021
Jiangsu Province Hospital
2015-2020
Brigham and Women's Hospital
2018-2020
Harvard University
2018-2020
Cisplatin (CDDP) treatment is one of the most predominant chemotherapeutic strategies for patients with gastric cancer (GC). A better understanding mechanisms CDDP resistance can greatly improve therapeutic efficacy in GC. Circular RNAs (circRNAs) are a class noncoding whose functions related to pathogenesis cancer, but, GC remains unknown. circAKT3 (hsa_circ_0000199, circRNA originating from exons 8, 9, 10, and 11 AKT3 gene) was identified by RNA sequencing verified quantitative reverse...
Acquired drug resistance is a constraining factor in clinical treatment of glioblastoma (GBM). However, the mechanisms chemoresponsive tumors acquire therapeutic remain poorly understood. Here, we aim to investigate whether temozolomide (TMZ) GBM was enhanced by long non-coding RNA SBF2 antisense 1 (lncRNA SBF2-AS1) enriched exosomes.LncSBF2-AS1 level TMZ-resistance or TMZ-sensitive tissues and cells were analyzed qRT-PCR FISH assays. A series vitro assay xenograft tumor models performed...
BackgroundAlthough temozolomide (TMZ) resistance is a significant clinical problem in glioblastoma (GBM), its underlying molecular mechanisms are poorly understood. In this study, we identified the role of exosomal microRNAs (miRNAs) from TMZ-resistant cells as important mediators chemoresistance GBM cells.MethodsExosomes were isolated and characterized via scanning electron microscopy (SEM). Expression levels miR-1238 cell lines their exosomes, tissues, sera evaluated by RT-qPCR. vitro vivo...
Gliomas are based on a genetic abnormality and present with dismal prognosis. MicroRNAs (miRNAs) considered to be important mediators of gene expression in glioma tissues.Real-time PCR was used analyze the microRNA-423-5p (miR-423-5p) human samples normal brain tissue. Apoptosis, cell cycle, proliferation, immunostaining, transwell, vitro 2D 3D migration, chemosensitivity assays were performed assess phenotypic changes cells overexpressing miRNA-423-5p. Western blotting determine inhibitor...
Exosomes are carriers of pro-tumorigenic factors that participate in glioblastoma (GBM) progression, and many fusion genes strong driver mutations neoplasia involved tumorigenesis. However, the ability to be transduced by exosomes is unknown. We characterized from GBM cells harbouring not PTPRZ1–MET (ZM fusion). also determined effect ZM exosomes) on pro-oncogenic secretions showed internalized recipient cells. In addition, we studied exosome-mediated intercellular communication...
Therapeutic application of microRNAs (miRNAs) in Wnt-driven glioma has been valuable; however, their specific roles and mechanisms have not completely investigated. Real-time quantitative PCR (RT-qPCR) was used to analyse the expression microRNA-129-5p (miR-129-5p) human samples. Cell-Counting Kit 8 (CCK-8), flow cytometry, EdU, angiogenesis, Transwell invasion, wound healing, vitro 3D migration neurosphere formation assays were employed assess role miR-129-5p glioblastoma multiforme (GBM)...
Rationale: Glioma stem-like cells (GSCs) contribute to temozolomide (TMZ) resistance in gliomas, although the mechanisms have not been delineated. Methods:In vitro functional experiments (colony formation assay, flow cytometric analysis, TUNEL assay) were used assess ability of extracellular vesicles (EVs) from hypoxic GSCs promote TMZ glioblastoma (GBM) cells. RNA sequencing and quantitative Reverse Transcription-PCR employed identify miRNA EVs. Chromatin immunoprecipitation assays...
Abstract Long noncoding RNAs (lncRNAs) are involved in glioma initiation and progression. Glioma stem cells (GSCs) essential for tumor initiation, maintenance, therapeutic resistance. However, the biological functions underlying mechanisms of lncRNAs GSCs remain poorly understood. Here, we identified that LINC00839 was overexpressed GSCs. A high level associated with GBM progression radiation METTL3-mediated m6A modification on enhanced its expression a YTHDF2-dependent manner....
// Ailiang Zeng 1 , Qi Hu Yanwei Liu 2 Zheng Wang Xiaoming Cui Rui Li Wei Yan Yongping You Department of Neurosurgery, The First Affiliated Hospital Nanjing Medical University, 210029, PR China Beijing Neurosurgical Institute, Capital 100050, Correspondence to: You, e-mail: yypl9@njmu.edu.cn Yan, neuro_yw@njmu.edu.cn Keywords: IDH1/2 mutation, Ki-67 labeling index, prognosis, glioma, molecular classification Received: March 24, 2015 Accepted: August 07, Published: 18, ABSTRACT current World...
Background/Aims: Glioma is one of the most devasting tumors and confers dismal prognosis. Long noncoding RNAs(lncRNAs) have emerged as important regulators in various including glioma. A classic lncRNA-H19, which found to be highly expressed human glioma tissues cell lines, associated with tumor progression thus predicating clinical outcomes patients. However, overall biological functions their mechanism H19 are not fully understood. Methods: Firstly, we analyzed alterations different grades...
Abstract Cisplatin (CDDP) is commonly used for gastric cancer (GC) chemotherapy. However, after several CDDP‐based treatment cycles, patients always acquire chemotherapy resistance, which limits the overall clinical efficacy of treatment. Clarification mechanisms responsible CDDP resistance required to improve therapeutic outcomes patients. Circular RNAs (circRNAs) are noncoding involved in pathogenesis cancer, although their role mechanism underlying GC remains unknown. In present study, we...
Glioblastoma (GBM) may arise from astrocytes through a multistep process involving progressive accumulation of mutations. We explored whether GBM-derived extracellular vesicles (EVs) facilitate neoplastic transformation and malignant growth astrocytes. utilized conditioned media (CM) cultured glioma cells, its sequential filtration, diverse cell-based assays, RNA sequencing, metabolic assays to compare the effects EV-containing EV-depleted CM. GBM EVs facilitated pre-transformed but not...
In this study, we demonstrate that bone mesenchymal stem cell (BMSC)-derived exosomes alter tumor phenotypes by delivering miR-512-5p. miR-512-5p was downregulated in glioblastoma tissues and cells, Jagged 1 (JAG1) the target gene of We clarified expression patterns JAG1 along with their interactions glioblastoma. Additionally, observed BMSC-derived could contain transport to cells vitro. exosomal inhibited proliferation induced cycle arrest suppressing expression. vivo assays validated...
Abstract Glioblastoma (GBM) is the most universal type of primary brain malignant tumour, and prognosis patients with GBM poor. S100A11 plays an essential role in tumour. However, molecular mechanism are not clear. Here, we found that was up‐regulated tissues higher expression indicated poor patients. Overexpression promoted cell growth, epithelial‐mesenchymal transition (EMT), migration, invasion generation glioma stem cells (GSCs), whereas its knockdown inhibited these activities. More...
As the most common cause of progressive cognitive decline in humans, Alzheimer's disease (AD) has been intensively studied, but mechanisms underlying its profound synaptic dysfunction remain unclear. Here we confirm that exposing wild-type mice to an enriched environment (EE) facilitates signaling hippocampus promotes long-term potentiation (LTP). Exposing kept standard housing soluble Aβ oligomers impairs LTP, EE can fully prevent this. Mechanistically, key molecular features benefit are...
Glioblastoma multiforme is the most common primary malignancy in brain and confers a uniformly poor prognosis. MicroRNAs have been shown to activate or inhibit tumorigenesis. Abnormalities p53 signaling pathway are found various cancers correlate with tumor formation. We examined expression of microRNA-141-3p (miR-141-3p) glioma different grades by analysis profiling databases clinical specimens. Cell proliferation flow cytometry assays were performed evaluate promotion miR-141-3p...
Temozolomide was recognized as the first-line therapy for glioblastoma to prolong survival of patients noticeably, while recent clinical studies found that some were not sensitive temozolomide treatment. The possible mechanisms seemed be methylguanine-DNA-methyltransferase (MGMT), mismatch repair, PARP, etc. And abnormal expression MGMT might most direct factor. In this study, we provide evidence Fstl1 plays a vital role in resistance by sequentially regulating DIP2A protein distribution,...
Numerous studies have reported that long noncoding RNA (lncRNA) dysregulation is involved in the progression of many malignant tumors, including glioma. The lncRNA ZNFX1 antisense 1 (ZFAS1) plays an oncogenic role various such as gastric cancer and hepatocellular carcinoma. However, underlying molecular mechanism ZFAS1 glioma has not been fully clarified. In this study, we found expression was upregulated both tissues cell lines. Functional experiments revealed promoted proliferation,...
Defective cholesterol biosynthesis in eye lens cells is often associated with cataracts; however, how genes involved are regulated remains unclear. Here, we show that Quaking (Qki) required for the transcriptional activation of lens. At transcriptome level, lens-specific Qki-deficient mice present downregulation pathway, resulting a significant reduction total level Mice Qki depletion epithelium display progressive accumulation protein aggregates, eventually leading to cataracts. Notably,...
CircRNAs are closely related to ferroptosis in gastric cancer cells; however, the mechanism by which circRNAs regulate carcinogenesis remains unknown. CircRNA-encoded novel peptides functional products translated from open reading frames (ORFs) within circular RNAs, demonstrating that not only serve as non-coding regulators but also have capacity encode biologically active peptides. Compared with noncancerous cells, cells greater iron requirements, and occurs response radiotherapy,...
Background: Gliomas result in the highest morbidity and mortality rates of intracranial primary central nervous system tumors because their aggressive growth characteristics high postoperative recurrence. They are characterized by genetic instability, intratumoral histopathological variability unpredictable clinical behavior patients. Proliferation is a key aspect progression malignant gliomas, complicating complete surgical resection enabling tumor regrowth further proliferation surviving...