A5101981458- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Radiopharmaceutical Chemistry and Applications
- SARS-CoV-2 and COVID-19 Research
- Cancer-related gene regulation
- SARS-CoV-2 detection and testing
- Cancer Research and Treatments
- Circular RNAs in diseases
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Pneumonia and Respiratory Infections
- Traumatic Brain Injury and Neurovascular Disturbances
- Protein Degradation and Inhibitors
- Renin-Angiotensin System Studies
- RNA Research and Splicing
- Bacterial Infections and Vaccines
- Advanced biosensing and bioanalysis techniques
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Pneumocystis jirovecii pneumonia detection and treatment
- Antioxidant Activity and Oxidative Stress
- Peroxisome Proliferator-Activated Receptors
- Traumatic Brain Injury Research
Nanchang University
2017-2025
Ganzhou People's Hospital
2017-2024
Fujian Provincial Cancer Hospital
2023
Fujian Medical University
2023
Nanjing Medical University
2014-2016
Jiangsu Province Hospital
2015-2016
Hebei Agricultural University
2016
Beijing VDJBio (China)
2010-2015
Methodist Hospital
2012
Baylor College of Medicine
2012
// Wenkang Luan 1,* , Yingyi Wang Xincheng Chen Yan Shi Jiajia 1 Junxia Zhang Jin Qian 2 Ri Li Tao Wenjin Wei Qi Hu Ning Liu and Yongping You Department of Neurosurgery, The First Affiliated Hospital Nanjing Medical University, Nanjing, China People’s Xuancheng City, Anhui, * These authors contributed equally to this work Correspondence to: You, email: Keywords : let-7a microRNA, c-Myc, hnRNPA1, PKM2, glucose metabolism, aerobic glycolysis, glioma Received October 08, 2014 Accepted...
Enhancer of zeste homolog 2 (EZH2) is the catalytic unit polycomb repressive complex (PRC2) which epigenetically silences many genes involved in tumor-suppressive mechanisms via trimethylation lysine 27 histone H3 (H3K27me3). We recently found that overexpression EZH2 was associated with poor outcome glioblastoma (GBM). In this study, we examined antitumor effects inhibitor GSK343 on glioma cells vitro and vivo. The proliferation cell cycle measured. Wound healing assay transwell invasion...
// Dongfeng Han 1, * , Wenjin Wei Xincheng Chen Yaxuan Zhang Yingyi Wang 1 Junxia Xiefeng Tianfu Yu Qi Hu Ning Liu Yongping You Department of Neurosurgery, The First Affiliated Hospital Nanjing Medical University, 210029, China These authors have contributed equally to this work Correspondence to: You, e-mail: YYPL9@njmu.edu.cn Keywords: fenofibrate, PPARα, RelA, PKM2, Warburg effect Received: February 16, 2015 Accepted: June 19, Published: 30, 2015...
Lentiviral vectors have been used successfully to rapidly produce decigram quantities of active recombinant proteins in mammalian cell lines. To optimize the protein production platform, roles Ubiquitous Chromatin Opening Element (UCOE), an insulator, and selected promoters were evaluated based on efficiency stability foreign gene expression mediated by lentiviral vectors.Five vectors, pFIN-EF1α-GFP-2A-mCherH-WPRE containing EF1α promoter HS4 p'HR.cppt.3'1.2kb-UCOE-SFFV-eGFP SFFV UCOE,...
Angiotensin-(1-7) [Ang-(1-7)] is an endogenous, heptapeptide hormone acting through the Mas receptor (MasR), with antiproliferative and antiangiogenic properties. Recent studies have shown that Ang-(1-7) has action on lung adenocarcinoma cells prostate cancer cells. In this study, we report MasR levels were significantly upregulated in nasopharyngeal carcinoma (NPC) specimens NPC cell lines. Viral vector-mediated expression of dramatically suppressed proliferation migration vitro. These...
Objective Spontaneous hypertensive brainstem hemorrhage (HBSH) is characterized by sudden onset, rapid progression and poor prognosis. There has been a growing tendency of surgical treatment for HBSH. This study aimed to investigate outcomes potential factors associated with the prognosis robot-assisted drainage surgery HBSH treatment. Methods Patients from July 2016 March 2023 at single neurosurgery center were included divided into conservative group groups. Baseline clinical data,...
Fenofibrate, a fibric acid derivative, is known to possess lipid-lowering effects. Although fenofibrate-induced peroxisome proliferator-activated receptor alpha (PPARα) transcriptional activity has been reported exhibit anticancer effects, the underlying mechanisms are poorly understood. In this study, we investigated behind antiproliferative effects of fenofibrate in U87MG cells (human glioma cell line) using WST-8 Cell Proliferation Assay Kit. Furthermore, examined genome-wide gene...
// Yingyi Wang 1, * , Min 2, Wenjin Wei Dongfeng Han Xincheng Chen 1 Qi Hu Tianfu Yu Ning Liu Yongping You Junxia Zhang Department of Neurosurgery, The First Affiliated Hospital Nanjing Medical University, Nanjing, China 2 Radiology, These authors contributed equally to this work Correspondence to: Zhang, email: zjx2032@126.com Keywords: EZH2, miRNA, β-catenin, aerobic glycolysis, glioma Received: February 01, 2016 Accepted: June 12, Published: July ABSTRACT EZH2 is up-regulated in various...
Abstract Background Glucose metabolic reprogramming is a significant hallmark of malignant tumors including GBM. Previous studies suggest that microRNAs play key roles in modulating this process GBM cells. miR-181b acts as tumor suppressor miRNA influencing glioma tumorigenesis. Our previous results showed was down-regulated cells and tissues. Methods The extracellular acidification rate (ECAR), colony formation assay levels Glut1 PKM2 were measured to assess the glucose proliferation...
The pathogenesis and progression of GBM (glioblastoma), as one the most frequently occurring malignancies central nervous system, are regulated by several genes. BUB1 (budding uninhibited benzimidazoles 1) is a mitotic checkpoint that plays an important role in chromosome segregation well various tumors. However, its glioma unknown. current study discovered prominently elevated significant relationship between expression, high World Health Organization grade, poor prognosis patients....
ABSTRACT The gene encoding the 56-kDa protein of Orientia tsutsugamushi Shanxi was amplified by a nested PCR and cloned into expression vector pQE30. O. (Sxh56) expressed as fusion with His 6 -binding Escherichia coli deleting signal peptide-encoding sequence from 5′ end open reading frame. recombinant formed inclusion bodies when in E. M15. examined for reactivity mouse sera against three antigenic prototypes an immunoblot assay. Sxh56 reacted only to polyclonal antiserum Gilliam...
To evaluate the safety and immunogenicity of 23-valent pneumococcal polysaccharide vaccine (PPV23), a randomized, double-blind parallel controlled clinical trial was conducted in Yancheng, Jiangsu Province China. There were 1200 subjects randomized into 2 groups with 1:1 allocation. Subjects received 0.5 mL tested PPV23 or control by intramuscular injection deltoid, respectively. Results showed that seroconversion rates all 23 types except type 3 not significantly different between groups....
This study was designed to develop and validate a risk-prediction nomogram predict 6-month unfavorable prognosis in patients with traumatic brain-injured (TBI) undergoing primary decompressive craniectomy (DC). The clinical data of 391 TBI DC who were admitted from 2012 2020 reviewed, which 274 enrolled the training group, while 117 internal validation randomly. external sets containing 80 obtained another hospital. Independent predictors analyzed using multivariate logistic regression....
Abstract Background To determine an appropriate dose of, and immunization schedule for, a vaccine SCoK against COVID‐19 for efficacy study; herein, we conducted randomized controlled trials to assess the immunogenicity safety of this in adults. Methods These randomized, double‐blind, placebo‐controlled phase 1 2 were Binhai District, Yan City, Jiangsu Province, China. Younger older adult participants sequentially recruited into different groups be intramuscularly administered 20 or 40 μg...
Several receptor proteins of Cry toxin have been previously identified, including cadherin-like, aminopeptidase N, and alkaline phosphatase. In the present work, a novel binding protein, V-ATPase subunit A (HpVAA), was identified in Holotricia parallela larvae characterized. We performed reverse transcription-polymerase chain reaction rapid amplification cDNA ends technology to obtain full-length hpvaa. Sequencing analysis showed that open reading frame hpvaa (GenBank accession No. KU497557)...