A5080809635- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Plant and animal studies
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Sphingolipid Metabolism and Signaling
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Ion-surface interactions and analysis
- Seed Germination and Physiology
- Mass Spectrometry Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Plant nutrient uptake and metabolism
- Aquatic Ecosystems and Phytoplankton Dynamics
- Plant Gene Expression Analysis
- Botanical Research and Chemistry
- Plant Virus Research Studies
- Algal biology and biofuel production
- Polysaccharides and Plant Cell Walls
- GABA and Rice Research
- Light effects on plants
- Plant-Derived Bioactive Compounds
Teikyo University
2016-2025
Barnyardgrass (Echinochloa crus-galli) is a pernicious weed in agricultural fields worldwide. The molecular mechanisms underlying its success the absence of human intervention are presently unknown. Here we report draft genome sequence hexaploid species E. crus-galli, i.e., 1.27 Gb assembly representing 90.7% predicted size. An extremely large repertoire genes encoding cytochrome P450 monooxygenases and glutathione S-transferases associated with detoxification found. Two gene clusters...
Abstract Hydrogen peroxide (H 2 O ) is a ubiquitous signal regulating many biological processes, including innate immunity, in all eukaryotes. However, it remains largely unknown that how transcription factors directly sense H Here, we report rice basic/helix-loop-helix factor bHLH25 senses to confer resistance multiple diseases caused by fungi or bacteria. Upon pathogen attack, plants increase the production of , which oxidizes at methionine 256 nucleus. Oxidized represses miR397b...
Lysin motif (LysM) receptor-like kinase CERK1 is a co-receptor essential for plant immune responses against carbohydrate microbe-associated molecular patterns (MAMPs). Concerning the immediate downstream signaling components of CERK1, cytoplasmic kinases such as PBL27 and other RLCK VII members have been reported to regulate positively. In this study, we report that novel CERK1-interacting E3 ubiquitin ligase, PUB4, also involved in regulation MAMP-triggered responses. Knockout PUB4 resulted...
Summary N‐acetyltransferase HOOKLESS1 (HLS1) in Arabidopsis, a recognized pivotal factor hook formation, is also implicated multifaceted developmental processes including plastochron and leaf senescence. However, its molecular function remains to be elucidated. We utilized LC‐MS for plant hormone quantification implemented series of genetic physiological approaches analyze HLS1 functions. Our findings indicate that functions tissues distant from expression sites the regulation apical...
Recovery of the root system following physical damage is an essential issue for plant survival. An injured able to regenerate by increases in lateral (LR) number and acceleration growth. The horticultural technique pruning (root cutting) application this response a common garden controlling Although widely used, molecular mechanisms underlying subsequent changes are poorly understood. In study, was employed as model study regeneration. Notably, LR defects wild-type plants treated with...
The plant hormone abscisic acid (ABA) and the jasmonic related-compound 12-oxo-phytodienoic (OPDA) play crucial roles in seed development, dormancy, germination. However, a lack of suitable techniques for visualising hormones has restricted investigation their biological mechanisms. In present study, desorption electrospray ionisation-imaging mass spectrometry (DESI-IMS), powerful tool metabolites tissues, was used to visualise ABA OPDA immature Phaseolus vulgaris L. sections. spectra, peak...
Plants have the regenerative ability to reconnect cut organs, which is physiologically important survive severe tissue damage. The organs utilized as grafting combine two different individuals. Callus formation at graft junction facilitates organ attachment and vascular reconnection. While it well documented that local wounding signals provoke callus formation, how differentially regulated each end remains elusive. Here, we report activity asymmetrical between top bottom ends by differential...
Abscisic acid (ABA) and 12-oxo-phytodienoic (OPDA) play crucial roles in seed development. However, because of their low ionization efficiencies, visualization by matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) has been difficult. In this study, we used on-tissue chemical derivatization (OTCD) with the reagent Girard's T (GirT) MALDI-IMS to visualize ABA OPDA.Immature Phaseolus vulgaris L. seeds were homogenized, frozen homogenate sections prepared using a...
Rice produces low-molecular-weight antimicrobial compounds known as phytoalexins, in response to not only pathogen attack but also abiotic stresses including ultraviolet (UV) irradiation. phytoalexins are composed of diterpenoids and a flavonoid. Recent studies have indicated that endogenous jasmonyl-l-isoleucine (JA-Ile) is necessarily required for the production diterpenoid blast-infected or CuCl2-treated rice leaves. However, JA-Ile accumulation flavonoid phytoalexin, sakuranetin. Here,...
Abstract Jasmonic acid (JA) regulates plant growth, development and stress responses. Coronatine insensitive 1 (COI1) jasmonate zinc-finger inflorescence meristem-domain (JAZ) proteins form a receptor complex for jasmonoyl-l-isoleucine, biologically active of JA. Three COIs (OsCOI1a, OsCOI1b OsCOI2) are encoded in the rice genome. In present study, we generated mutants each COI gene using genome editing to reveal physiological functions three COIs. The oscoi2 mutants, but not oscoi1a oscoi1b...
Glucosylceramide (GlcCer) is composed of a glucose moiety attached to ceramides (Cer). GlcCer was reported reduce the intestinal impairments in vivo and suppress vitro inflammation. In this study, clarify effect on vivo, we compared effects shiitake mushroom-derived (fungal GlcCer) formation aberrant crypt foci (ACF) colon inflammation with those fungal Cer having same GlcCer. Fungal GlcCer- or Cer-supplemented diets significantly suppressed ACF 1,2-dimethylhydrazine (DMH)-treated mice....
Jasmonic acid (JA) and its biologically active form jasmonoyl- L -isoleucine (JA-Ile) regulate defense responses to various environmental stresses developmental processes in plants. JA JA-Ile are synthesized from α-linolenic acids derived membrane lipids via 12-oxo-phytodienoic (OPDA). In the presence of JA-Ile, COI1 receptor physically interacts with JAZ repressors, leading their degradation, resulting transcription JA-responsive genes by MYC factors. Although biosynthesis is conserved...
Abstract Root development is essential for plant survival. The lack of carotenoid biosynthesis in the phytoene desaturase 3 (pds3) mutant results short primary roots (PRs) and reduced lateral root formation. In this study, we showed that short-term inhibition PDS by fluridone suppresses PR growth wild type, but to a lesser extent auxin mutants Arabidopsis (Arabidopsis thaliana). Such an activity increased endogenous indole-3-acetic acid levels, promoted signaling, partially complemented...
ABSTRACT Glucosylceramides and ceramides with 8E 8Z isomers of the long chain base are found in plants. These have been difficult to quantify separately using liquid chromatography–tandem mass spectrometry (LC–MS/MS) because same retention time, their precursor product ions m/z values, plant ceramide standards not commercially available. Here we tested trial separations various ODS columns prepared generated by human glucocerebrosidase (imiglucerase) available glucosylceramide as substrates....
A facile and efficient method has been developed for the optical resolution of racemic jasmonic acid (JA) on a relatively large scale was successfully utilized preparation optically pure (+)-JA (-)-JA. We indicated that lower growth inhibitory activity than (-)-JA in rice seedling test confirmed line with an earlier observation their respective biologically-active forms, (+)-JA-Ile (-)-JA-Ile, show comparable activities. compared metabolism into respectively, JA-deficient cpm2, found...
L-3,4-dihydroxyphenylalanine (L-DOPA) is one of the important secondary metabolites plants and has been used for various purposes, such as in clinical treatment Parkinson's disease dopamine-responsive dystonia. In plants, L-DOPA a precursor many alkaloids, catecholamines, melanin; synthesis pathway similar to that mammals. acts an allelochemical, role several biological processes, stress response metabolism, plants. widely well dietary supplement or psychotropic drug, understanding...
Plant hormone signalling and the circadian clock have been implicated in transcriptional control of isoprene biosynthesis. To gain more insight into hormonal biosynthesis, present study measured plant concentrations jasmonic acid (JA)-treated leaves our previous model study, examined their relationship with gene expression synthase (IspS) transcription factors. Of hormones, IAA JA-Ile related factors (MYC2 SAUR21) were significantly correlated IspS expression. Concentrations cytokinins,...
Long-chain base phosphates (LCBPs) such as sphingosine-1-phosphate and phytosphingosine-1-phosphate function abscisic acid (ABA)-mediated signaling molecules that regulate stomatal closure in plants. Recently, a glycoside hydrolase family 1 (GH1) β-glucosidase, Os3BGlu6, was found to improve drought tolerance by rice, but the biochemical functions of Os3BGlu6 have remained unclear. Here we identified novel GH1 glucocerebrosidase (GCase) catalyzes hydrolysis glucosylceramide ceramide....
Abstract Root development is essential for plant survival. The lack of carotenoid biosynthesis in the phytoene desaturase 3 ( pds3 ) mutant results short primary roots (PR) and reduced lateral root (LR) formation. In this study, we show that short-term inhibition PDS by fluridone suppressed PR growth WT, but to a lesser extent auxin mutants. Such an activity increased endogenous indole-3-acetic acid (IAA) levels, promoted signaling, partially complemented deficient mutant, YUCCA 5 7 8 9...