A5114339921- Monoclonal and Polyclonal Antibodies Research
- Acute Kidney Injury Research
- Fibroblast Growth Factor Research
- Dialysis and Renal Disease Management
- Hemoglobinopathies and Related Disorders
- ATP Synthase and ATPases Research
- Blood groups and transfusion
- Chronic Kidney Disease and Diabetes
- Atherosclerosis and Cardiovascular Diseases
- interferon and immune responses
- Glycosylation and Glycoproteins Research
- Microfluidic and Capillary Electrophoresis Applications
- Antimicrobial Peptides and Activities
- S100 Proteins and Annexins
- Estrogen and related hormone effects
- Pharmacological Effects of Natural Compounds
- Prenatal Screening and Diagnostics
- RNA and protein synthesis mechanisms
- Phytochemistry and biological activity of medicinal plants
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Protein purification and stability
- Biosimilars and Bioanalytical Methods
- Retinal and Optic Conditions
Jilin Medical University
2011-2024
This study explores the role of succinate as a vaccine adjuvant, revealing its capacity to enhance neutrophil recruitment at immunization sites, which boosts B cell activation through receptor 1-interferon regulatory factor 5-B cell-activating (SUCNR1-IRF5-BAFF) signaling pathway. Results demonstrate succinate’s potential amplify vaccine-induced antibody responses, highlighting significance in immunological enhancement and offering new insights into adjuvant mechanisms action, particularly...
Abstract Background AKI is related to severe adverse outcomes and mortality with Coronavirus Disease 2019 (COVID-19) patients, that early diagnosed intervened imperative. Neutrophil gelatinase-associated lipocalin (NGAL) one of the most promising biomarkers for detection acute kidney injury (AKI), but current methods are inadequacy, so more rapid, convenient accuracy needed detect NGAL diagnosis AKI. Herein, we established a reliable lateral flow immunoassay (LFIA) based on europium...
Antler velvet polypeptide (VAP) is a prominent bioactive component of antler velvet. Whereas uncharacterized crude extracts have typically been used in pharmacological studies, this study, the was isolated and purified by acid water extraction, ethanol precipitation, ammonium sulfate fractionation chromatography, progressively. Human umbilical vein endothelial cells (HUVECs) were induced with H2O2 followed treatment. Cell viability evaluated MTT assay. The apoptosis detected fluorescence...
Aberrant signaling pathways leads to cancer initiation and progression. Both Notch PI3K/AKT are believed be involved in prostate cancer. How the interaction between two contributes progression androgen independence is still elusive.Prostate cells were grown RPMI 1,640 supplemented with 10% heat-inactivated fetal bovine serum (FBS) or charcoal-stripped (FCS), 1% penicillin-streptomycin 75 cm2 polystyrene flasks, maintained at 37 °C a humidified atmosphere 5% CO2 . Cell proliferation, invasion...
A large human natural single‑chain fragment variable (scFv) phage library was constructed based on Cre‑LoxP recombination, and used to successfully identify antibodies against proprotein convertase subtilisin/kexin type 9 (PCSK9). The derived from 400 blood samples, 30 bone marrow 10 cord samples healthy donors. Lymphocytes were isolated each sample cDNA synthesized using reverse transcription‑quantitative PCR. Two‑step overlap PCR then for scFv synthesis a LoxP peptide as the linker. gene...
D-dimer is an essential diagnostic index of thrombotic diseases. Since the existing anti-D-dimer antibodies vary in quality and specificity, a search for alternative required. The present study aimed to screen novel monoclonal antibody (mAb) against using light-initiated chemiluminescence assay (LiCA). In this work, mice were immunized with antigen prepared from human plasma by enzyme hydrolysis. After screening, mAb, DD 2G11, was obtained. results sodium dodecyl sulfate-polyacrylamide gel...
Purpose: The purpose of this study was to the extract Rumex root which had an inhibitory action on cell proliferation human leukemia line THP-1. Methods: combination percolation and extraction used separate main chemical compositions Rumex, MIT assay curve inhibition ratio. Results: petroleum ether acetone extracted from can all restrain THP-1 cells. Therefore, new theoretical basis about reasonable treatment acute is provided.
Objective: Fetomaternal hemorrhage (FMH) is an alloimmunization resulting caused by the incompatibility between fetal and maternal blood. For prevention of newborn haemolytic disease (HDN), it crucial to quantify amount fetomaternal hemorrhage. However, classical Kleihauer-Betke test (K-B test) for detecting limited experimental tools conditions not suitable routine clinical use. Consequently, method prenatal diagnosis applicable clinic a topic worthy further study. Therefore, worthwhile...
Abstract Background: AKI is related to severe adverse outcomes and mortality with Coronavirus Infection Disease 2019 (COVID-19) patients, that early diagnosed intervened imperative. Neutrophil gelatinase-associated lipocalin (NGAL) one of the most promising biomarkers for detection acute kidney injury (AKI), but current methods are inadequacy, so more rapid, convenient accuracy needed detect NGAL diagnosis AKI. Herein, we established a reliable lateral flow immunoassay based on europium...