A5034779097- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Birth, Development, and Health
- Metabolism and Genetic Disorders
- Single-cell and spatial transcriptomics
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Renal and related cancers
- Mitochondrial Function and Pathology
- Bioinformatics and Genomic Networks
- Amino Acid Enzymes and Metabolism
- Health, Environment, Cognitive Aging
- Cancer, Hypoxia, and Metabolism
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nitric Oxide and Endothelin Effects
- Microbial metabolism and enzyme function
- Cell Image Analysis Techniques
- Chemical Reactions and Isotopes
- Cancer Genomics and Diagnostics
- Pregnancy and Medication Impact
- Cardiovascular Disease and Adiposity
- Diet and metabolism studies
- Advanced Fluorescence Microscopy Techniques
- Atherosclerosis and Cardiovascular Diseases
- Systemic Sclerosis and Related Diseases
University of Michigan
1993-2023
Michigan Medicine
2022-2023
Kidney organoids are a promising model to study kidney disease, but their use is constrained by limited knowledge of functional protein expression profile. Here, we define the organoid proteome and transcriptome trajectories over culture duration upon exposure TNFα, cytokine stressor. Older increase deposition extracellular matrix decrease glomerular proteins. Single cell integration reveals that most changes localize podocytes, tubular stromal cells. TNFα treatment results in 322...
Minakawa, Akihiro; Berthier, Celine C.; Fischer, Matthew; El Saghir, Jamal; Vega-Warner, V.; Menon, Rajasree; Hartman, John R.; Eichinger, Felix H.; Schaub, Jennifer A.; Kretzler, Matthias; Harder, L. Author Information
Abstract Kidney organoids are a promising model to study kidney disease, but use is constrained by limited knowledge of their functional protein expression profile. We aimed define the organoid proteome and transcriptome trajectories over culture duration upon exposure TNFα, cytokine stressor. Older increased deposition extracellular matrix decreased glomerular proteins. Single cell integration revealed that most changes localized podocytes, tubular stromal cells. TNFα-treatment effected 320...
Minakawa, Akihiro; Fischer, Matthew; Saghir, Jamal El; He, Chenchen; Vega-Warner, V.; Hartman, John R.; Eichinger, Felix H.; Schaub, Jennifer A.; Pennathur, Subramaniam; Kretzler, Matthias; Harder, L. Author Information