A5083069127- Appendicitis Diagnosis and Management
- Pancreatitis Pathology and Treatment
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Pancreatic function and diabetes
- Sepsis Diagnosis and Treatment
- Diabetes Treatment and Management
- Chronic Kidney Disease and Diabetes
- Pleural and Pulmonary Diseases
- RNA modifications and cancer
- Streptococcal Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- Advanced MRI Techniques and Applications
- Birth, Development, and Health
- Cardiac, Anesthesia and Surgical Outcomes
- Protease and Inhibitor Mechanisms
- Cerebrovascular and Carotid Artery Diseases
- Abdominal Surgery and Complications
- Health and Medical Studies
- Molecular Biology Techniques and Applications
- S100 Proteins and Annexins
- Amino Acid Enzymes and Metabolism
- Bacterial Identification and Susceptibility Testing
- Heat shock proteins research
- Diverse Scientific Research Studies
University Medical Center Hamburg-Eppendorf
2024
Johns Hopkins University
2024
University Medical Center
2024
Sorbonne Université
2024
Universität Hamburg
2024
Inserm
2024
Aarhus University
2022-2024
Aarhus School of Architecture
2024
Heidelberg University
2024
University Hospital Heidelberg
2024
Minimal change disease and primary focal segmental glomerulosclerosis in adults, along with idiopathic nephrotic syndrome children, are immune-mediated podocytopathies that lead to syndrome. Autoantibodies targeting nephrin have been found patients minimal disease, but their clinical pathophysiological roles unclear.
BACKGROUND: SGLT2 (sodium-glucose cotransporter 2) inhibitors (SGLT2i) can protect the kidneys and heart, but underlying mechanism remains poorly understood. METHODS: To gain insights on primary effects of SGLT2i that are not confounded by pathophysiologic processes or secondary to improvement SGLT2i, we performed an in-depth proteomics, phosphoproteomics, metabolomics analysis integrating signatures from multiple metabolic organs body fluids after 1 week treatment nondiabetic as well...
Abstract Early prognostic evaluation of abdominal sepsis is desirable to select high-risk patients for more aggressive therapeutic procedures and provide objective classification the severity disease. The reliability Mannheim peritonitis index was assessed its predictive power different populations examined in a study 2003 from seven centres three European countries. prevalence risk factors varied considerably between groups. For threshold score 26, sensitivity 86 (range 54–98) per cent,...
Abstract Mesenchymal stem cells (MSC) are multipotent with great potential in therapy, reflected by more than 500 MSC-based clinical trials registered the NIH. MSC derived from multiple tissues but require invasive harvesting and imply donor-to-donor variability. Embryonic cell-derived (ESC-MSC) may provide an alternative, how similar they to ex vivo is unknown. Here we performed depth characterization of human ESC-MSC, comparing them bone marrow-derived (BM-MSC) as well embryonic (hESC)...
RNA editing encompasses a post-transcriptional process in which the genomically templated sequence is enzymatically altered and introduces modified base into edited transcript. Mammalian C-to-U represents distinct subtype of modification, whose prototype intestinal apolipoprotein B mRNA, mediated by catalytic deaminase Apobec-1. However, genome-wide identification, tissue-specificity functional implications Apobec-1-mediated remain incompletely explored. Deep sequencing, data filtering...
Glucocorticoids exert rapid nongenomic effects by several mechanisms including the activation of a membrane-bound glucocorticoid receptor (mGR). Here, we report first proteomic study on mGR BSA-conjugated cortisol (Cort-BSA). A subset target proteins in data set was validated Western blot and found them responding to three additional cell lines, indicating conserved effect cells originating from different tissues. Changes proteome treated CCRF-CEM leukemia were associated with early...
Dynamic range limitations are challenging to proteomics, particularly in clinical samples. Affinity proteomics partially overcomes this, yet suffers from dependence on reagent quality. SOMAscan, an aptamer-based platform for over 1000 proteins, avoids that issue using nucleic acid binders. Targets include low expressed proteins not easily accessible by other approaches. Here we report the potential of SOMAscan study differently sourced mesenchymal stem cells (MSC) comparison LC-MS/MS and RNA...
Due to high prevalence and slow progression of prostate cancer, primary prevention appears be attractive strategy for its eradication. During the last decade, curcumin (diferuloylmethane), a natural compound from root turmeric (Curcuma longa), was described as potent chemopreventive agent. Curcumin exhibits anti-inflammatory, anticarcinogenic, antiproliferative, antiangiogenic, antioxidant properties in various cancer cell models. This study designed identify proteins involved anticancer...
Significance The mechanisms blocking Ca 2+ influx in mitosis are complex and involve a decrease stable endoplasmic reticulum (ER)–plasma membrane (PM) contact sites degradation of the ER sensor stromal interaction molecule 1 (STIM1) but not its phosphorylation. This challenges current view that STIM1 phosphorylation is essential for mitotic store-operated entry inhibition sheds light on dynamics ER–PM mitosis.
Despite broad application of magnetic nanoparticles in biomedicine and electronics, only a few vivo studies on biocompatibility are available. In this study, toxicity metal oxide the respiratory system was examined by single intratracheal instillation mice. Bronchoalveolar lavage fluid (BALF) samples were collected for proteome analyses LC–MS/MS, testing Fe3O4 doped with increasing amounts cobalt (Fe3O4, CoFe2O4 an iron to ratio 5:1, 3:1, 1:3, Co3O4) at two doses (54 μg, 162 μg per animal)...
The lipid kinase VPS34 orchestrates autophagy, endocytosis, and metabolism is implicated in cancer metabolic disease. proximal tubule the kidney a key organ that controls reabsorption of nutrients such as fatty acids, amino sugars, proteins. Here, by combining metabolomics, proteomics, phosphoproteomics analyses with functional superresolution imaging assays mice an inducible deficiency tubular cells, we revealed controlled metabolome tubule. In addition to inhibiting pinocytosis depletion...
Kidney organoids are a promising model to study kidney disease, but their use is constrained by limited knowledge of functional protein expression profile. Here, we define the organoid proteome and transcriptome trajectories over culture duration upon exposure TNFα, cytokine stressor. Older increase deposition extracellular matrix decrease glomerular proteins. Single cell integration reveals that most changes localize podocytes, tubular stromal cells. TNFα treatment results in 322...
Introduction: The increasing number of enterococcal infections in hospitals and reports about the development resistance these bacteria make it necessary to review their importance as copathogens secondary peritonitis. Materials Methods: A prospective randomized controlled trial on primary therapy peritonitis was carried out six centers comparing cephalosporin-based antibiotic acylaminopenicillin-based therapy. Results: Enterococci were only cultured 6 110 cases from abdomen found 5...
Dynamic Contrast-Enhanced MR Angiography from the Distal Aorta to Ankle Joint with a Step-by-Step TechniqueA. Huber1, A. Heuck1, Baur1, T. Helmberger1, Waggershauser1, Billing2, M. Heiss2, R. Petsch1 3 and Reiser1Audio Available | Share
Abdominal sepsis due to intestinal leakage of endogenous gut bacteria is a life-threatening condition. In healthy individuals, T lymphocytes have essential functions in balancing the immune response commensal flora.To determine how shape process diffuse faecal peritonitis.In colon ascendens stent peritonitis (CASP), clinically relevant mouse model peritonitis, kinetics systemic cell activation were investigated by assessment markers. CD4(+) cells then depleted with monoclonal antibodies, and...
Mesenchymal stem/stromal cells (MSCs) are self-renewing multipotent with regenerative, secretory and immunomodulatory capabilities that beneficial for the treatment of various diseases. To avoid issues come using tissue-derived MSCs in therapy, may be generated by differentiation human embryonic stems (hESCs) culture. However, changes occur during process have not been comprehensively characterized. Here, we combined transcriptome, proteome phosphoproteome profiling to perform an in-depth,...
Moving-Table MR Angiography of the Peripheral Runoff Vessels: Comparison Body Coil and Dedicated Phased Array SystemsA. Huber1, J. Scheidler1, B. Wintersperger1, A. Baur1, M. Schmidt2, Requardt2, N. Holzknecht1, T. Helmberger1, Billing3 Reiser1Audio Available | Share
Abstract The glucocorticoid (GC) cortisol, the main mediator of hypothalamic‐pituitary‐adrenal axis has many implications in metabolism, stress response and immune system. Its function is mediated via binding to receptor (GR), a member superfamily ligand‐activated nuclear hormone receptors. activity ligated GR results from its as transcription factor elements (GREs). Two‐dimensional gel electrophoresis with DIGE (fluorescence difference electrophoresis) technology was applied study effects...
The effects of cortisol (CORT) on resting and lipopolysaccharide (LPS)-activated monocyte-derived THP-1 macrophages were investigated by proteomics. Forty-seven proteins found to be modulated, 20 CORT, 11 LPS, 16 CORT LPS. Cortisol-sensitive chaperones cytoskeletal mostly repressed. HCLS1, MGN, MX1 new identified under the transcriptional control this steroid CORT-sensitive variants MX1, SYWC IFIT3 found. FKBP51, a known target gene, showed strongest response synergism with In macrophages,...