A5101563093- HIV-related health complications and treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes and associated disorders
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- Cerebral Palsy and Movement Disorders
- Electronic Health Records Systems
- Spinal Cord Injury Research
- Adipose Tissue and Metabolism
- COVID-19 Clinical Research Studies
- T-cell and B-cell Immunology
- Eicosanoids and Hypertension Pharmacology
- Ethics in Clinical Research
- HIV Research and Treatment
- Family and Disability Support Research
- Apelin-related biomedical research
- Immunotherapy and Immune Responses
- Healthcare Quality and Management
- Immunodeficiency and Autoimmune Disorders
- Sodium Intake and Health
- Health Policy Implementation Science
- Patient Safety and Medication Errors
- Data Quality and Management
- Fatty Acid Research and Health
- SARS-CoV-2 and COVID-19 Research
Vanderbilt University Medical Center
2019-2025
Metro South Health
2015-2024
Vanderbilt University
2019-2023
Princess Alexandra Hospital
2015-2016
Chronic T cell activation and accelerated immune senescence are hallmarks of HIV infection, which may contribute to the increased risk cardiometabolic diseases in people living with (PLWH). lymphocytes play a central role modulating adipose tissue inflammation and, by extension, adipocyte energy storage release. Here, we assessed CD4+ CD8+ profiles subcutaneous (SAT) blood non-diabetic (n=9; fasting glucose [FBG]=126mg/dL) PLWH, addition non- pre-diabetic, HIV-negative controls (n=8). SAT...
Persistent systemic inflammation is associated with an elevated risk of cardiometabolic diseases. However, the characteristics innate and adaptive immune systems in individuals who develop these conditions remain poorly defined. Doublets, or cell-cell complexes, are routinely eliminated from flow cytometric other phenotyping analyses, which limits our understanding their relationship to disease states. Using well-characterized clinical cohorts, including participants controlled human...
Persons with HIV are at increased risk for diabetes mellitus compared individuals without HIV. Adipose tissue is an important regulator of glucose and lipid metabolism, adipose T cells modulate local inflammatory responses and, by extension, adipocyte function. have a high proportion CX3CR1+ GPR56+ CD57+ (C-G-C+) CD4+ in tissue, subset which cytomegalovirus specific, whereas but predominantly CD69+ cells. C-G-C+ cell subsets demonstrate higher receptor clonality the same blood, potentially...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with increased morbidity and mortality in solid organ transplant (SOT) recipients. Despite exclusion from SARS-CoV-2 vaccine clinical trials, these individuals were identified as high-risk prioritized for vaccination public health guidelines.
Persons with HIV (PWH) on long-term antiretroviral therapy (ART) have a higher incidence and prevalence of cardiometabolic diseases attributed, in part, to persistent inflammation despite viral suppression. In addition traditional risk factors, immune responses co-infections such as cytomegalovirus (CMV) may play an unappreciated role comorbidities offer new potential therapeutic targets subgroup individuals. We assessed the relationship CX3CR1 + , GPR56 CD57 +/- T cells (termed CGC )...
Introduction Subcutaneous adipose tissue (SAT) is a critical regulator of systemic metabolic homeostasis. Persons with HIV (PWH) have an increased risk diseases and significant alterations in the SAT immune environment compared general population. Methods We generated comprehensive single-cell multi-omic atlas to characterize cellular compositional transcriptional changes 59 PWH across spectrum health. Results Glucose intolerance was associated lipid-associated macrophages, CD4 + CD8 T...
Abstract Context Cardiometabolic diseases are common in persons with HIV (PWH) on antiretroviral therapy (ART), which has been attributed to preferential lipid storage visceral adipose tissue (VAT) compared subcutaneous (SAT). However, the relationship of SAT-specific cellular and molecular programs VAT volume is poorly understood PWH. Objective We characterized SAT cell-type specific composition transcriptional that associated greater PWH contemporary ART. Methods enrolled long-term ART a...
Introduction The rising global burden of metabolic disease impacts the control endemic tuberculosis (TB) in many regions, as persons with diabetes mellitus (DM) are up to three times more likely develop active TB than those without DM. Active can also promote glucose intolerance during both acute infection and over a longer term, potentially driven by aspects immune response. Identifying patients have persistent hyperglycemia following treatment would enable closer monitoring care, an...
Pediatric hematopoietic cell transplant (HCT) recipients exhibit poor serologic responses to influenza vaccination early after transplant. To facilitate the optimization of timing, we sought identify B- and T-cell subpopulations associated with vaccine immunogenicity in this population. We used mass cytometry phenotype peripheral blood mononuclear cells collected from pediatric HCT enrolled a multicenter trial comparing high- standard-dose formulations over 3 seasons (2016-2019). fit linear...
Background: In the absence of reliable predictive equations, indirect calorimetry (IC) remains gold standard for assessing energy requirements after spinal cord injury (SCI), but it is typically confined to a research setting. The purpose this study assess feasibility and acceptability implementing IC into routine clinical care in an Australian SCI rehabilitation facility. Methods: Bedside (canopy hood) was performed, patients completed questionnaire (open-ended; yes/no; 5-point Likert...
Objective To utilise a concept mapping process to identify key opportunities for electronic medical record (EMR) optimisation allied health professionals (AHPs). Methods A total of 26 participants (allied managers, clinicians and healthcare consumers) completed the process, which included generating statements, then subsequently sorting all statements into groups, also ranking each statement importance changeability (0 = not important/changeable, 4 extremely important/changeable)....
Electronic standard order sets automate the ordering of specific treatment, testing, and investigative protocols by physicians. These tools may help reduce unwarranted clinical variation improve health care efficiency. Despite their routine implementation within electronic medical records (EMRs), little is understood about how they are used what factors influence adoption in practice.
Introduction A vaccine against influenza is available seasonally but not 100% effective. predictor of successful seroconversion in adults an increase activated circulating T follicular helper (cTfh) cells after vaccination. However, the impact repeated annual vaccinations on long-term protection and seasonal efficacy remains unclear. Methods In this study, we examined cell receptor (TCR) repertoire transcriptional profile vaccine-induced expanded cTfh individuals who received sequential...
Abstract An increased risk of cardiometabolic disease accompanies persistent systemic inflammation. Yet, the innate and adaptive immune system features in persons who develop these conditions remain poorly defined. Doublets, or cell-cell complexes, are routinely eliminated from flow cytometric other phenotyping analyses, which limits our understanding their relationship to states. Using well-characterized clinical cohorts, including participants with controlled HIV as a model for chronic...
Background: Electronic medical records (EMRs) have the potential to improve and streamline quality safety of patient care. Harnessing full benefits EMR implementation depends on utilisation advanced features, defined as “mature usage.” At present, little is known about maturity usage by allied health professionals (AHPs). Objective: To examine current use AHPs explore perceived barriers mature optimisation. Method: were recruited from three services. Participants completed a 27-question...
Subcutaneous white adipose tissue (scWAT) is a critical regulator of systemic metabolic homeostasis. HIV infection accompanied by alterations in scWAT immune cells, which may contribute to the increased risk for cardiometabolic diseases persons with (PWH) and serve as model investigate cellular relationships. We generated largest molecular atlas humans date from 91 individuals using paired single-cell transcriptomic proteogenomics determine profile non-diabetic, pre-diabetic, diabetic PWH,...
While antiretroviral therapy (ART) has proven effective in suppressing viremia and disease progression among people living with human immunodeficiency virus (HIV; PLWH), suboptimal CD4+ T cell reconstitution remains a major obstacle nearly 30% of ART-treated individuals. Epidemiological studies demonstrate that obesity, or body mass index (BMI) ≥ 30 kg/m2, is positively correlated greater recovery PLWH on ART. Leptin known immunomodulator produced proportion to fat increased obese...
An increased risk of cardiometabolic disease accompanies persistent systemic inflammation. Yet, the features innate and adaptive immune system in persons who develop these conditions remain poorly defined. Doublets, or cell-cell complexes, are routinely eliminated from flow cytometric other phenotyping analyses, which limits our understanding their relationship to states. Using well-characterized cohorts, including a group individuals with treated HIV as model for chronic inflammation cell...
<sec> <title>BACKGROUND</title> Electronic standard order sets automate the ordering of specific treatment, testing, and investigative protocols by physicians. These tools may help reduce unwarranted clinical variation improve health care efficiency. Despite their routine implementation within electronic medical records (EMRs), little is understood about how they are used what factors influence adoption in practice. </sec> <title>OBJECTIVE</title> This study aims to (1) describe patterns use...
Abstract Background Pediatric hematopoietic cell transplant recipients (PHCTRs) have poor serologic responses to influenza vaccination. While absolute numbers of circulating B and T cells are important predictors response, our goal was identify subpopulations associated with vaccine immunogenicity. Methods We conducted a secondary analysis PHCTRs enrolled in multicenter randomized controlled trial spanning three seasons (2016–2019). Peripheral blood mononuclear were collected before...
Persons with HIV (PWH) are at high risk for diabetes mellitus. Adipose tissue T cells a central regulator of inflammation and adipocyte function, the known establishment reservoir in adipose could contribute to metabolic dysregulation. Here, we show that CD4+ cell subsets increased diabetic PWH, specifically CD69+ CD69lo CD57+ GPR56+ CX3CR1+, clonally expanded shared receptors, suggesting common lineage. We observed both CX3CR1+ have transcriptomes consistent TH1 profile diabetics versus TH2...