A5029261402- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Digital Imaging for Blood Diseases
- Respiratory viral infections research
- Sodium Intake and Health
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Plant Disease Resistance and Genetics
- Adipose Tissue and Metabolism
- Animal Virus Infections Studies
- HIV Research and Treatment
- Plant and Fungal Interactions Research
- Eicosanoids and Hypertension Pharmacology
- Peroxisome Proliferator-Activated Receptors
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- HIV-related health complications and treatments
- Genomics and Phylogenetic Studies
- Bioinformatics and Genomic Networks
- Gut microbiota and health
- Computational Drug Discovery Methods
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Asthma and respiratory diseases
- Infectious Diseases and Mycology
Vanderbilt University Medical Center
2019-2025
Vanderbilt University
2021-2024
University of Georgia
2014-2023
Institute of Bioinformatics
2019
J. Craig Venter Institute
2012-2017
Emory University
2016-2017
Abstract The Omicron variant of SARS‐CoV‐2 achieved worldwide dominance in late 2021. Early work suggests that infections caused by the may be less severe than those Delta variant. We sought to compare clinical outcomes these two strains, confirmed whole genome sequencing, over a short period time, from respiratory samples collected positive patients at large medical center. found significantly morbidity, including admission hospital and requirement for oxygen supplementation, mortality
The genera Aspergillus and Penicillium include some of the most beneficial as well harmful fungal species such penicillin-producer chrysogenum human pathogen fumigatus, respectively. Their mitochondrial genomic sequences may hold vital clues into mechanisms their evolution, population genetics, biology, yet only a handful these genomes have been fully sequenced annotated. Here we report complete sequence annotation six three species: A. clavatus, oryzae, flavus, Neosartorya fischeri (A....
Salt sensitivity of blood pressure is an independent predictor cardiovascular morbidity and mortality. The exact mechanism by which salt intake increases risk unknown. We previously found that sodium entry into antigen-presenting cells (APCs) via the amiloride-sensitive epithelial channel EnaC (epithelial channel) leads to formation IsoLGs (isolevuglandins) release proinflammatory cytokines activate T modulate salt-sensitive hypertension. In current study, we hypothesized ENaC-dependent APCs...
Genetic variations across the SARS-CoV-2 genome may influence transmissibility of virus and host's anti-viral immune response, in turn affecting frequency variants over time. In this study, we examined adjacent amino acid polymorphisms nucleocapsid (R203K/G204R) that arose on background spike D614G change describe how strains harboring these changes became dominant circulating globally.Deep-sequencing data from public databases clinical samples were analyzed to identify map genetic...
Iron plays a critical role in survival and virulence of the opportunistic pathogen Aspergillus fumigatus. Two transcription factors, GATA-factor SreA bZip-factor HapX oppositely monitor iron homeostasis with activating acquisition pathways (e.g., siderophores) shutting down consumptive (and SreA) during starvation conditions whereas negatively regulates corresponding sufficiency. Recently non-ribosomal peptide, hexadehydroastechrome (HAS; tryptophan-derived (III)-complex), has been found...
The soil fungus Rhizoctonia solani is a pathogen of agricultural crops. Here, we report on the 51,705,945 bp draft consensus genome sequence R. strain Rhs1AP. A comprehensive understanding heterokaryotic complexity and organization may provide insight into plant disease ecology adaptive behavior fungus.
Invasive aspergillosis (IA) due to Aspergillus fumigatus is a major cause of mortality in immunocompromised patients. The discovery highly fertile strains A. opened the possibility merge classical and contemporary genetics address key questions about this pathogen. merger involves sexual recombination, selection desired traits, genomics identify any associated loci. We constructed isogenic pair with opposite mating types used them investigate whether type virulence find genetic loci involved...
Chronic malaria is a major public health problem and significant challenge for disease eradication efforts. Despite its importance, the biological factors underpinning chronic are not fully understood. Recent studies have shown that host metabolic state can influence pathogenesis transmission, but role in chronicity known. Here, with goal of identifying distinct modifications metabolite profiles acute versus malaria, metabolomics was performed on plasma from Plasmodium-infected humans...
We developed a metatranscriptomics method that can simultaneously capture the respiratory virome, microbiome, and host response directly from low biomass samples. Using nasal swab samples, we RNA virome with sufficient sequencing depth required to assemble complete genomes. find surprisingly high frequency of syncytial virus (RSV) coronavirus (CoV) in healthy children, RSV-A RSV-B co-detections children symptomatic RSV. In addition, have identified commensal pathogenic bacteria fungi at...
BACKGROUND: The mechanisms by which salt increases blood pressure in people with sensitivity remain unclear. Our previous studies found that high sodium enters antigen-presenting cells (APCs) via the epithelial channel and leads to production of isolevuglandins hypertension. In current mechanistic clinical study, we hypothesized channel–dependent isolevuglandin-adduct formation APCs is regulated epoxyeicosatrienoic acids (EETs) salt-sensitive hypertension humans. METHODS: Salt was assessed...
Persistent systemic inflammation is associated with an elevated risk of cardiometabolic diseases. However, the characteristics innate and adaptive immune systems in individuals who develop these conditions remain poorly defined. Doublets, or cell-cell complexes, are routinely eliminated from flow cytometric other phenotyping analyses, which limits our understanding their relationship to disease states. Using well-characterized clinical cohorts, including participants controlled human...
SUMMARY Plasmodium knowlesi has risen in importance as a zoonotic parasite that been causing regular episodes of malaria throughout South East Asia. The P. genome sequence generated 2008 highlighted and confirmed many similarities differences species, including global view several multigene families, such the large SICAvar family encoding variant antigens known schizont-infected cell agglutination proteins. However, repetitive DNA sequences are bane any project, this other projects have not...
ABSTRACT To elucidate the molecular mechanisms that manifest lung abnormalities during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, we performed whole-transcriptome sequencing of autopsies from 31 patients with COVID-19 and ten uninfected controls. Using metatranscriptomics, identified existence two distinct signatures lethal COVID-19. The dominant ‘classical’ signature (n=23) showed upregulation unfolded protein response, steroid biosynthesis complement...
Abstract Background Genetic variations across the SARS-CoV-2 genome may influence transmissibility of virus and host’s anti-viral immune response, in turn affecting frequency variants over-time. In this study, we examined adjacent amino acid polymorphisms nucleocapsid (R203K/G204R) that arose on background spike D614G change describe how strains harboring these changes became dominant circulating globally. Methods Deep sequencing data from public databases clinical samples were analyzed to...
Cytochrome P450 epoxygenase Cyp2c44, a murine epoxyeicosatrienoic acid (EET)-producing enzyme, promotes insulin sensitivity, and Cyp2c44−/− mice show hepatic resistance. Because resistance leads to lipid accumulation hyperlipidemia, we hypothesized that Cyp2c44 regulates metabolism. Standard chow diet (SCD)-fed male had significantly decreased EET levels increased plasma compared with wild-type mice. We showed membrane localization of the FA transporter 2 (FATP2) total unsaturated fatty...
ABSTRACT Plasmodium coatneyi is a protozoan parasite species that causes simian malaria and an excellent model for studying disease caused by the human parasite, P. falciparum . Here we report complete (nontelomeric) genome sequence of Hackeri generated application only Pacific Biosciences RS II (PacBio II) single-molecule real-time (SMRT) high-resolution technology assembly using Hierarchical Genome Assembly Process (HGAP). This first reported to use PacBio technology. approach has proven...
Little is known about the relationships between symptomatic early severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral load and upper airway mucosal gene expression immune response. To examine association of SARS-CoV-2 with expression, we profiled host transcriptome from nasopharyngeal swab samples 68 adults symptomatic, mild-to-moderate disease 19 (COVID-19). We measured using reverse transcription-quantitative PCR (RT-qPCR). then examined detected in all recovered >80% genome...
Persons with HIV (PWH) on long-term antiretroviral therapy (ART) have a higher incidence and prevalence of cardiometabolic diseases attributed, in part, to persistent inflammation despite viral suppression. In addition traditional risk factors, immune responses co-infections such as cytomegalovirus (CMV) may play an unappreciated role comorbidities offer new potential therapeutic targets subgroup individuals. We assessed the relationship CX3CR1 + , GPR56 CD57 +/- T cells (termed CGC )...
R hizoctonia solani agglutinin ( RSA ) is a 15.5‐kDa lectin accumulated in the mycelium and sclerotia of soil born plant pathogenic fungus . Although it considered to serve as storage protein implicated fungal insecticidal activity, its physiological role remains unclear result lack any structure/function relationship information. Glycan arrays showed that displays high selectivity towards terminal nonreducing N ‐acetylgalactosamine residues. We determined amino acid sequence also crystal...
Mild to severe anaemia is a common complication of malaria that caused in part by insufficient erythropoiesis the bone marrow. This study used systems biology evaluate transcriptional and alterations cell populations marrow during Plasmodium cynomolgi infection rhesus macaques (a model vivax malaria) may affect erythropoiesis. An appropriate erythropoietic response did not occur compensate for acute despite an increase erythropoietin levels. During this period, there were significant...
We describe a multi-omic approach to understanding the effects that anti-malarial drug pyrimethamine has on immune physiology in rhesus macaques (Macaca mulatta). Whole blood and bone marrow RNA-Seq plasma metabolome profiles (each with over 15,000 features) have been generated for five naïve individuals at up seven time-points before, during after three rounds of administration. Linear modelling Bayesian network analyses are both considered, alongside investigations impact statistical...