A5025484839- Renin-Angiotensin System Studies
- Receptor Mechanisms and Signaling
- ATP Synthase and ATPases Research
- Hormonal Regulation and Hypertension
- Protein Degradation and Inhibitors
- Retinoids in leukemia and cellular processes
- Classical Antiquity Studies
- Hippo pathway signaling and YAP/TAZ
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Biotin and Related Studies
- Theatre and Performance Studies
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Mast cells and histamine
- Computational Drug Discovery Methods
- RNA Interference and Gene Delivery
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Contemporary and Historical Greek Studies
- Alzheimer's disease research and treatments
- RNA Research and Splicing
- Click Chemistry and Applications
- interferon and immune responses
- PARP inhibition in cancer therapy
Charité - Universitätsmedizin Berlin
2008-2017
Universidade Federal de Minas Gerais
2017
Jena University Hospital
2017
Institute of Pharmacology
2012
The angiotensin type 2 receptor (AT2R) and the MAS are receptors of protective arm renin-angiotensin system. They mediate strikingly similar actions. Moreover, in various studies, AT2R antagonists blocked effects agonists vice versa. Such cross-inhibition may indicate heterodimerization these receptors. Therefore, this study investigated molecular functional interplay between AT2R. Molecular interactions were assessed by fluorescence resonance energy transfer cross correlation spectroscopy...
Objectives Inhibition of (pro)renin receptor activation was demonstrated to inhibit or even abolish the development end-organ damage in animal models. The new renin inhibitor, aliskiren, markedly increases plasma concentration ligands prorenin and patients. effects inhibitors on signal transduction cascade are currently unknown. Results Our results indicate that were equally potent by decreasing mRNA, increasing phosphatidylinositol-3 kinase p85α mRNA augmenting viable cell number,...
Stroke is one of the major medical burdens in industrialized countries. Animal experiments indicate that blockade angiotensin AT1 receptor (AT1R) improves neurological outcome after cerebral ischemia. These protective effects are partially mediated by AT2 (AT2R). The transcription factor promyelocytic leukemia zinc finger (PLZF) was identified as a direct adapter protein AT2R. Furthermore, our group able to demonstrate PLZF also directly binds and mediates human (pro)renin [(P)RR] which...
Genetic factors strongly contribute to the pathogenesis of sporadic Alzheimer's disease (AD). Nevertheless, genome-wide association studies only yielded single nucleotide polymorphism loci moderate importance. In contrast, microsatellite repeats are functionally less characterized structures within our genomes. Previous work has shown that endothelin-converting enzyme-1 (ECE-1) is able reduce amyloid β content. Here we demonstrate a CpG-CA repeat human ECE-1c promoter highly polymorphic,...
The (pro)renin receptor [(P)RR] is crucial for cardio-renal pathophysiology. distinct molecular mechanisms of this are still incompletely understood. (P)RR able to interact with different signalling proteins such as promyelocytic leukemia zinc finger protein (PLZF) and Wnt receptors. Moreover, domains the essential V-ATPase activity. V-ATPase- Wnt-mediated effects imply constitutive, i.e., (pro)renin-independent functions (P)RR. Regarding ligand-dependent signalling, role prorenin...
The (pro)renin receptor ((P)RR) signaling is involved in different pathophysiologies ranging from cardiorenal end-organ damage via diabetic retinopathy to tumorigenesis. We have previously shown that the transcription factor promyelocytic leukemia zinc finger (PLZF) an adaptor protein of (P)RR. Furthermore, recent publications suggest major functions (P)RR are mediated ligand-independently by its transmembrane and intracellular part, which acts as accessory V-ATPases. transcriptome...
Background/Aims: Putative in vitro-in vivo correlations of pharmacokinetic (PK) parameters are regarded as a prerequisite to filter hits derived from high-throughput screening (HTS) approaches for subsequent murine PK studies. Methods: In this study, we assessed stabilities rat and human microsomes 121 compounds an early, academic drug discovery programme targeting the (pro)renin receptor correlated respective data with single-dose, 22 administered intravenously rats. Results: After...
This paper presents a re-reading of Aristophanes’s comedy Ekklesiazusai (Assembly Women). It shows that this play exhibits the aporias binary gender order, which had evolved in classical fifth-century Athens, along with other dualisms typical period, such as opposition pólis and oíkos. The argues Aristophanes negotiates these against background changing epistemological conditions fifth century, i. e. establishment “principle bivalence”. From perspective theater literary history, development...
The angiotensin AT2-receptor (AT2R) and the receptor MAS share a strinkingly similar spectrum of signaling mechanisms protective, physiological actions. Furthermore, cross-inhibition by respective antagonists has been observed. Therefore we hypothesised that physical interaction between these two receptors may exist. HEK-293 cells were transfected with vectors encoding or AT2R fused in C-terminus fluorophores CFP YFP for FRET GFP mCherry FCCS. photobleaching was used to detect, whether are...