A5066356468- Plant and Fungal Interactions Research
- Parasitic Infections and Diagnostics
- Crustacean biology and ecology
- Invertebrate Immune Response Mechanisms
- Insect and Pesticide Research
- Plant Disease Resistance and Genetics
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Insect Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Plant responses to water stress
- Animal Virus Infections Studies
- Toxoplasma gondii Research Studies
- Fungal and yeast genetics research
- Cellular transport and secretion
- Phytoplasmas and Hemiptera pathogens
- Marine Biology and Environmental Chemistry
- Mosquito-borne diseases and control
- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
Southwest University
2016-2023
Ministry of Agriculture and Rural Affairs
2023
Citrus Research Institute
2023
Research Institute of Resource Insects
2023
State Key Laboratory of Silkworm Genomic Biology
2016-2022
All microsporidia possess a unique, highly specialized invasion mechanism that involves the polar tube and spore wall. The interaction between wall proteins (SWPs) (PTPs) in formation, arrangement, orderly orientation, function of remains to be determined. This study was undertaken examine protein interactions Nosema bombycis SWP7 (NbSWP7), NbSWP9, PTPs. Coimmunoprecipitation, liquid chromatography-tandem mass spectrometry (LC-MS/MS), yeast two-hybrid data demonstrated but not NbSWP7,...
Enterocytozoon hepatopenaei (EHP) is the pathogen of hepatopancreatic microsporidiosis (HPM) in shrimp. The diseased shrimp Litopenaeus vannamei exhibits a slow growth syndrome, which causes severe economic losses. Herein, 4D label-free quantitative proteomics was employed to analyze hepatopancreas L. with light (EHPptp2 < 103 copies/50 ng hpDNA, L group) and heavy > 104 H load EHP better understand pathogenesis HPM. Exactly 786 (L 1056 (H differentially expressed proteins (DEPs) versus...
Microsporidia are a big group of single-celled obligate intracellular organisms infecting most animals and some protozoans. These minimalist eukaryotes lack numerous genes in metabolism vesicle trafficking. Here, we demonstrated that the spore wall protein NbSWP12 microsporidium Nosema bombycis belongs to Bin/Amphiphysin/Rvs (BAR) family can specifically bind with phosphatidylinositol 3-phosphate [Ptdlns(3)P]. Since Ptdlns(3)P is involved endosomal biogenesis trafficking, heterologous...
The polar tube protein is the major component of tube, and can specifically locate on microsporidia plays an important role in invasion host cell. In this study, we analyzed potential O- Nglycosylation sites 1 from Nosema bombycis. NbPTP1 was successfully cloned to eukaryotic expression vector pMT/Bip/V5-His A, involved V5 His tags. After transfection, gene could be efficiently expressed Drosophila S2 cells. addition, Lectin blotting beta elimination analysis showed that cells...
Microsporidia are obligate intracellular eukaryotic parasites that have significantly reduced genomes and lost most of their introns. In the current study, we characterized a gene in microsporidia Nosema bombycis, annotated as TRAPα (HNbTRAPα). The homologous functional component ER translocon facilitates initiation protein translocation substrate-specific manner, which is conserved animals but absent from fungi. coding sequence HNbTRAPα consists 2226 nucleotides, longer than majority...
Abstract Citrus exocortis viroid (CEVd) and citrus bark cracking (CBCVd) are two important viroids that infect plants frequently occur as mixed infections in orchards. However, the mechanism of antagonism between remains unclear. The CEVd/CBCVd–citron system small RNA sequencing (sRNA‐seq) were used to study antagonism. When CBCVd was inoculated before CEVd, CEVd titre significantly reduced symptoms attenuated. Viroid‐derived sRNAs (vd‐sRNAs) from predominantly 21‐nucleotide (nt) 22‐nt...
Abstract The pacific whiteleg shrimp ( Litopenaeus vannamei ) and the crayfish Procambarus clarkii are most productive aquatic animals in world. prevalence of microsporidium Enterocytozoon hepatopenaei (EHP), an intracellular spore-forming unicellular parasite which leading retarded growth L. , has caused severe economic losses farming country. In this study, we found that wild P. living ponds with EHP outbreak, excreted white faeces after temporary laboratory culture. hepatopancreas (HP)...