A5102351316- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Clostridium difficile and Clostridium perfringens research
- Nematode management and characterization studies
- Animal Virus Infections Studies
- Tryptophan and brain disorders
- Fungal Biology and Applications
- Metabolomics and Mass Spectrometry Studies
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Fungal Diseases
- Respiratory Support and Mechanisms
- interferon and immune responses
- Plant-Microbe Interactions and Immunity
- Herpesvirus Infections and Treatments
- Human-Animal Interaction Studies
- Diet and metabolism studies
- Gynecological conditions and treatments
- Antifungal resistance and susceptibility
- Plant biochemistry and biosynthesis
- Uterine Myomas and Treatments
- Probiotics and Fermented Foods
- Microbial Fuel Cells and Bioremediation
- Reproductive System and Pregnancy
- Ferroptosis and cancer prognosis
- Respiratory viral infections research
Yunnan University
2020-2025
Kunming Institute of Zoology
2022
Chinese Academy of Sciences
2022
Abstract To explore potential intermediate host of a novel coronavirus is vital to rapidly control continuous COVID-19 spread. We found genomic and evolutionary evidences the occurrence 2019-nCoV-like (named as Pangolin-CoV) from dead Malayan Pangolins. Pangolin-CoV 91.02% 90.55% identical at whole genome level 2019-nCoV BatCoV RaTG13, respectively. lowest common ancestor RaTG13. The S1 protein much more closely related than Five key amino-acid residues involved in interaction with human...
Dietary selection and intake affect the survival health of mammals under extreme environmental conditions. It has been suggested that dietary composition is a key driver gut microbiota variation; however, how respond to seasonal changes natural conditions remains poorly understood. Sequencing plant trnL (UAA) region 16S rRNA gene analysis were employed determine in freely grazing yaks on Tibetan plateau. was more diverse winter than summer, while Gramineae Rosaceae consumed frequently all...
Abstract SARS-CoV-2 is the cause of COVID-19. It infects multiple organs including respiratory tract and gut. Dynamic changes regional microbiomes in infected adults are largely unknown. Here, we performed longitudinal analyses throat anal swabs from 35 COVID-19 19 healthy adult controls, as well 10 non-COVID-19 patients with other diseases, by 16 S rRNA gene sequencing. The results showed a partitioning into 3-4 categories based on microbial community types (I-IV) both sites. bacterial...
Tibetan pigs (TPs) can adapt to the extreme environments in plateau implicated by their self-genome signals, but little is known about roles of gut microbiota host adaption. Here, we reconstructed 8210 metagenome-assembled genomes from TPs (n = 65) living high-altitude and low-altitude captive (87 China-CPs 200 Europe-EPs) that were clustered into 1050 species-level genome bins (SGBs) at threshold 95% average nucleotide identity. 73.47% SGBs represented new species. The microbial community...
In the late December of 2019, an epidemic pneumonia (announced recently as Corona Virus Disease (COVID-19)) outbreak in city Wuhan China and widely spreads all over world. According to authoritative statistics, COVID-19 has caused more than 40,000 laboratory-confirmed infections with 1000 deaths by 12 February 2020 is still increasing. It was a novel identified coronavirus 2019-nCoV (renamed this virus severe acute respiratory syndrome 2, SARS-CoV-2). Released complete genomes 2019-nCoVs...
Here, we report hierarchical functions of a putative short-chain dehydrogenase/reductase gene Ao274 for arthrobotrisin biosynthesis in nematode-trapping fungus Arthrobotrys oligospora via an enhanced promoter. Transcriptional analysis revealed that exhibited much higher transcriptional levels than all other genes the biosynthetic pathway. We generated four mutants two types with varying reductions transcription through swap experiments. Target isolation and structural elucidation ten novel...
Here we explain the energy mechanism behind Warburg effect of aerobic glycolysis, which has been unsolved for a hundred years. We found that fungal cells can engage in extracellular Fenton reactions share central carbon metabolism with cancer produce effect. Fungal also undergo significantly reducing intracellular ATP levels and allocating large amounts oxygen reactions. The use glycolysis reaction be common phenomenon nature, as is metabolic pathway occurs every cell. development divided...
The gut microbiome has significant effects on healthy aging and aging-related diseases, whether in humans or nonhuman primates. However, little is known about the divergence convergence of microbial diversity between primates during aging, which limits their applicability for studying microbiome's role human health aging. Here, we performed 16S rRNA gene sequencing analysis captive rhesus macaques (Macaca mulatta) compared this data set with other freely available sets containing four...
Knowledge about coronaviruses (CoVs) with furin cleavage sites is extremely limited, although these mediate the hydrolysis of glycoproteins in plasma membranes required for MERS-CoV or SARS-CoV-2 to enter cells and infect humans. Thus, we have examined global epidemiology evolutionary history 248 other CoVs 86 diversified that been detected 24 animal hosts 28 countries since 1954. Besides SARS-CoV-2, two five known humans (HCoV-OC43 HCoV-HKU1) also sites. In addition, human enteric...
Thermomyces dupontii harbors two P450 paralogs (P450S and P450L) in the gene cluster for biosynthesis of prenylated indole alkaloids (PIAs) correponding iron chelators with P450L assigned as one protein containing a CYP like domain fused FAD-binding domain-containing oxidoreductase. Genetic manipulation metabolic profile analysis indicated both P450S were involved transforming simple PIAs to their corresponding chelators. Moreover, is responsible bolstering complex PIAs, reinforcing...
Tryptophan and its derived metabolites have been assumed to play important roles in the development survival of organisms. However, links tryptophan temperature change remained largely cryptic. Here we presented that a class prenyl indole alkaloids biosynthesized from dramatically accumulated thermophilic fungus
The formation of the trapping device induced by nematodes has been assumed as an indicator for a switch from saprophytic to predacious lifestyles nematode-trapping fungi. However, fungal nematocidal activity is not completely synonymous with trap formation. We found that predominant fungus Arthrobotrys oligospora harbored rare NRPS (Ao415) gene cluster was mainly distributed in Ao415 putatively encodes protein unique domain organization, distinct other NRPSs Mutation two key biosynthetic...
The discovery of eukaryotes with an iron overload phenotype offers promising insights for developing treatments and strategies human diseases caused by genetic defects. Under nutrient-limited conditions, Arthrobotrys oligospora other nematode-trapping fungi (NTF) can differentiate their mycelia into specialized structures to capture prey as a nutritional source. Recent work has shown that siderophore deficiency worsens the discrepancies between trapping device development fungal carnivorous...
Sesquiterpenyl epoxy-cyclohexenoids (SECs) that depend on a polyketide synthase-terpenoid synthase (PKS-TPS) pathway are widely distributed in plant pathogenic fungi. However, the biosynthesis and function of acetylated SECs still remained cryptic. Here, we identified AOL_s00215g 273 (273) was responsible for acetylation Arthrobotrys oligospora via construction Δ273, which were absent major antibacterial nonacetylated accumulated. Mutant Δ273 displayed increased trap formation, nematicidal...
Intrauterine adhesions (IUAs) have caused serious harm to women's reproductive health. Although emerging evidence has linked intrauterine microbiome gynecological diseases, the association of with IUA, remains unknown. We performed metagenome-wide association, metabolomics, and transcriptomics studies on IUA non-IUA uteri adult rats identify IUA-associated microbial species, which affected uterine metabolites endometrial transcriptions. A rat model was used one side duplex uterus undergoing...
The predominant nematode-trapping fungus Arthrobotrys oligospora harbors a unique polyketide synthase-prenyltransferase (PKS-PTS) gene cluster AOL_s00215g responsible for the biosynthesis of sesquiterpenyl epoxy-cyclohexenoids (SECs) that are involved in regulation fungal growth, adhesive trap formation, antibacterial activity, and soil colonization. However, function one rare (AOL_s00215g275 (275)) embedded has remained cryptic. Here, we constructed two mutants with disruption 275...
Our previous study reported that seminaturally occurring arthrocolins A to C with unprecedented carbon skeletons could restore the antifungal activity of fluconazole against fluconazole-resistant Candida albicans. Here, we showed synergized fluconazole, reducing minimum and dramatically augmenting survivals 293T human cells nematode Caenorhabditis elegans infected C. Mechanistically, can induce fungal membrane permeability arthrocolins, leading intracellular were critical combination therapy...
Abstract SARS-CoV-2 infects multiple organs including the respiratory tract and gut. Whether regional microbiomes are disturbed significantly to affect disease progression of COVID-19 is largely unknown. To address this question, we performed cross-sectional longitudinal analyses throat anal swabs from 35 adults 15 controls by 16S rRNA gene sequencing. The results allowed a partitioning patients into 3-4 categories (I-IV) with distinct microbial community types in both sites. Lower-diversity...
Abstract Choosing the right and appropriate amount of substances in a changing environment is essential for growth survival all species. We found that elevated oxygen levels significantly enhanced iron-rich trapping devices nematode-trapping fungus (NTF), Arthrobotrys oligospora . Interestingly, genomic analysis revealed NTFs lacked coq7 , key gene involved ubiquinol (UQ) biosynthesis iron chelation. Notably, A. utilizes UQ instead low-aerobic respiration. Transcriptional, metabolic,...
Abstract Background Children are less susceptible to COVID-19 and manifests lower morbidity mortality after infection, for which a multitude of mechanisms may be proposed. Whether the normal development gut-airway microbiome is affected by has not been evaluated. Results We demonstrate that alters respiratory gut children. Alteration was divergent between tract gut, albeit dysbiosis dominated genus Pseudomonas sustained up 25–58 days in different individuals. The distortion persisted 7/8...
Abstract The gut microbiota is essential for host health and survival. Here, using samples from animals living in the Qinghai-Tibetan Plateau, we recovered 119,568 metagenome-assembled genomes (MAGs) that were clustered into 19,251 species-level genome bins (SGBs) of which most represent novel species. We present a mechanism shaping mammalian microbiomes ancestral founder bacteria (AFB) as core skeleton recurring lineage-specific gains microbial species are transferred frequently among...
Abstract Children are less susceptible to COVID-19 and manifests lower morbidity mortality after infection, for which a multitude of mechanisms may be proposed. Whether the normal development gut-airway microbiome is affected by has not been evaluated. We demonstrate that alters respiratory gut children. Alteration was divergent between tract gut, albeit dysbiosis dominated genus Pseudomonas sustained up 25-58 days in different individuals. The distortion persisted 7/8 children at least...