A5072910282- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Pain Mechanisms and Treatments
- Intracerebral and Subarachnoid Hemorrhage Research
- Neuroscience and Neuropharmacology Research
- Schizophrenia research and treatment
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Neurotransmitter Receptor Influence on Behavior
- Chemical Synthesis and Analysis
- Antimicrobial Peptides and Activities
- Biological Activity of Diterpenoids and Biflavonoids
- Tryptophan and brain disorders
- Nicotinic Acetylcholine Receptors Study
- Pharmacological Effects of Natural Compounds
- Peptidase Inhibition and Analysis
- Diet and metabolism studies
- Analytical Chemistry and Chromatography
- Ion channel regulation and function
- Inflammatory mediators and NSAID effects
- Allergic Rhinitis and Sensitization
- Protein Hydrolysis and Bioactive Peptides
- Neurological Complications and Syndromes
- Natural product bioactivities and synthesis
Saitama Medical University
2024
Social Insurance Saitama Chuo Hospital
2024
Kitasato University
2024
Tokyo Women's Medical University
2016-2024
Daiichi Sankyo (Germany)
2024
Daiichi-Sankyo (South Korea)
2024
Fukuyama University
2014-2024
Boston Scientific (Netherlands)
2024
Seikei University
2014-2023
Pharmac
2020-2022
Abstract Wnt-5a is a representative ligand that activates β-catenin-independent pathway in the Wnt signaling. Although abnormal activation of β-catenin-dependent often observed human cancer, relationship between and tumorigenesis not clear. We sought to clarify how involved aggressiveness gastric cancer. Abnormal expression was 71 237 cancer cases by means immunohistochemistry. The positivity correlated with advanced stages poor prognosis had abilities stimulate cell migration invasion...
Abstract: Brain microglia are a major source of inflammatory cytokines, such as tumor necrosis factor‐α (TNF‐α), which have been implicated in the progression neurodegenerative diseases. Recently, were revealed to be highly responsive ATP, is released from nerve terminals, activated immune cells, or damaged cells. It not clear, however, whether ATP can regulate TNF‐α secretion microglia. Here we demonstrate that potently stimulates release, resulting mRNA expression rat cultured brain The...
Liver nodules smaller than 25 mm in diameter (19 hepatocellular carcinomas [HCCs] and nine adenomatous hyperplastic [AHNs]) were examined with color Doppler flow imaging hepatic angiography. Angiography revealed a tumor vessel eight (42%) of the 19 HCCs, while an arterial pulsating afferent 10 (53%) HCCs but none AHNs. In addition, constant-flow efferent continuing to portal branch 15 (79%) or both observed. Therefore, this series, was value distinguishing between these two lesions.
The mast cell plays a pivotal role in initiating allergic inflammation by secreting several cytokines including TNF-alpha, addition to granule mediators such as histamine. Anti-allergic drugs azelastine prevent immediate-type hypersensitivity inhibiting degranulation, well blocking histamine H1 receptors. However, their effects on cytokine release from cells remain unknown. In rat RBL-2H3 line, inhibited Ag- and ionomycin-induced TNF-alpha with IC50 values of 25.7 +/- 3.4 microM 1.66 0.45...
We previously described that recombinant interleukin-1beta (IL-1beta) induced the significant release of substance P (SP) via a cyclooxygenase (COX) pathway in primary cultured rat dorsal root ganglion (DRG) cells. In present study, we examined involvement two types phospholipase A2 (PLA2) enzymes, which lie upstream COX prostanoid-generating pathway, IL-1beta-induced SP from DRG The expression type IIA secretory PLA2 (sPLA2 -IIA) mRNA was undetectable by ribonuclease protection assay...
Abstract To clarify the molecular mechanism of substance P (SP) release from dorsal root ganglion (DRG) neurons, we investigated involvement several intracellular effectors in regulation SP evoked by capsaicin, potassium or/and bradykinin. Bradykinin‐evoked cultured adult rat DRG neurons was attenuated either mitogen‐activated protein kinase (MEK) inhibitor (U0126) or cycloheximide. As long‐term exposure to bradykinin (3 h) resulted extracellular signal‐regulated (ERK) phosphorylation at an...
A technique of covering the proximal nerve stump (PNS) has been reported as a preventative method or treatment for neuroma. However, its detailed pain relief mechanism remains unknown. We created silicone tube model in which PNS rat sciatic was introduced into tube, whereas controls had no tube. The score autotomy observed group lower than that control at 3 days to 2 weeks after surgery, suggested pain-like behavior inhibitory action. To elucidate inhibition, immunohistochemistry and...
The claudin family comprises four‐pass transmembrane proteins involved in the formation of tight junctions (TJs). Relatively recently, domain containing (CLDND) 1, also known as claudin‐25, was identified a novel member family. In present study, we revealed that adult murine brain, CLDND1 is abundant cerebellum among common sites intracerebral hemorrhage. Thus, dynamics after cerebellar hemorrhage were examined. Both mRNA and protein levels transiently decreased at 24 hr hemorrhagic insult....
Abstract Objectives Gadolinium (Gd) affects microglial polarization during remyelination. We previously reported that the suppression of proinflammatory microglia was neuroprotective in intracerebral haemorrhage (ICH). The objective present study to investigate effects Gd on and neuronal injury after ICH. Methods intraperitoneally administered ICH mice prepared by an intrastriatal microinjection collagenase type VII. M1, 2a, b c evaluated real-time PCR using respective markers. Changes...
In the chronic phase after intracerebral hemorrhage (ICH), aftereffect-associated lowering of motivation burdens many patients; however, pathogenic mechanism is unclear. Here, we revealed for first time that indoleamine 2, 3-dioxygenase (IDO) expression and enzyme activity are increased in collagenase-induced murine ICH model. IDO a rate-limiting situated at beginning kynurenine pathway converts tryptophan, source serotonin (5-hydroxytryptamine; 5-HT), to kynurenine. this study, showed...