A5057339710- Genetic Neurodegenerative Diseases
- Neuroscience and Neuropharmacology Research
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Intracerebral and Subarachnoid Hemorrhage Research
- Receptor Mechanisms and Signaling
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Ubiquitin and proteasome pathways
- Sulfur Compounds in Biology
- Lysosomal Storage Disorders Research
- Nuclear Receptors and Signaling
- Amino Acid Enzymes and Metabolism
- Neuropeptides and Animal Physiology
- Neurotransmitter Receptor Influence on Behavior
- Logic, Reasoning, and Knowledge
- Ion Transport and Channel Regulation
- Sirtuins and Resveratrol in Medicine
- RNA regulation and disease
- Extracellular vesicles in disease
- Pharmacological Receptor Mechanisms and Effects
- Nicotinic Acetylcholine Receptors Study
- Logic, programming, and type systems
- Advanced Algebra and Logic
Kumamoto University
2016-2025
Himeji Dokkyo University
2022-2025
Niigata University
1999-2024
Pharmac
2022-2023
Shibaura Institute of Technology
2020
Tokyo Medical and Dental University
2019
Hiroshima University
2007-2018
The University of Tokyo
2008-2016
Juntendo University
2016
National Center for Global Health and Medicine
2016
The taxonomic relationships among 56 strains of 16 species the genus Bacillus were studied by deoxyribonucleic acid (DNA)-DNA hybridization. In general, no significant DNA homology was detected between two different species, except for a group consisting B. subtilis, amyloliquefaciens, licheniformis, and pumilus another including cereus thuringiensis. Species former related, but they independent each other as their homologies 19% or less. indexes three thuringiensis to T, so far tested,...
Spinocerebellar ataxia type 14 (SCA14) is an autosomal dominant neurodegenerative disease caused by mutations in protein kinase Cgamma (PKCgamma). Interestingly, 18 of 22 are concentrated the C1 domain, which binds diacylglycerol and necessary for translocation regulation PKCgamma activity. To determine effect these on function pathology SCA14, we investigated enzymological properties mutant PKCgammas. We found that wild-type PKCgamma, but not domain mutants, inhibits Ca2+ influx response to...
Cerebellar Purkinje cells (PCs) express a large amount of the γ isoform protein kinase C (PKCγ) and modest level PKCα. The PKCγ is involved in pruning climbing fiber (CF) synapses from developing PCs, PKCα plays critical role long-term depression (LTD) at parallel (PF)-PC synapses. Moreover, PKC signaling PCs negatively modulates nonselective transient receptor potential cation channel type 3 (TRPC3), opening which elicits slow EPSCs PF-PC Autosomal dominant spinocerebellar ataxia 14 (SCA14)...
Autophagy-lysosome proteolysis is classified into macroautophagy (MA), microautophagy (mA) and chaperone-mediated autophagy (CMA). In contrast to MA CMA, mA have been mainly studied in yeast. 2011, mammalian was identified as a pathway deliver cytosolic proteins multivesicular bodies. However, its molecular mechanism quite different from yeast mA. Using cell-based method evaluate we revealed that rapamycin, an activator of mA, significantly activated Although rapamycin activates MA, also by...
We previously demonstrated that a synthetic retinoic acid receptor agonist, Am80, attenuated intracerebral hemorrhage (ICH)‐induced neuropathological changes and neurological dysfunction. Because inflammatory events are among the prominent features of ICH pathology affected by this study investigated potential involvement proinflammatory cytokines/chemokines in effect Am80 on ICH. induced collagenase injection into mouse striatum caused upregulation mRNAs for interleukin (IL)−1β, tumor...
Background Chaperone-mediated autophagy (CMA) is a selective autophagy-lysosome protein degradation pathway. The role of CMA in normal neuronal functions and neural disease pathogenesis remains unclear, part because there no available method to monitor activity at the single-cell level. Methodology/Principal Findings We sought establish monitoring by visualizing translocation substrates from cytosol lysosomes using HaloTag (HT) system. GAPDH, substrate, was fused HT (GAPDH-HT); this...
Polyglutamine diseases, including spinocerebellar ataxia type 3 (SCA3), are caused by CAG repeat expansions that encode abnormally long glutamine repeats in the respective disease proteins. While mechanisms underlying neurodegeneration remain uncertain, evidence supports a proteotoxic role for mutant protein dictated part specific genetic and context. To further define pathogenic SCA3, we generated mouse model which expansion of 82 was inserted into murine locus homologous recombination....
Spinocerebellar ataxia type 14 (SCA14) is an autosomal dominant neurodegenerative disease characterized by various symptoms including cerebellar ataxia. Recently, several missense mutations in the protein kinase Cγ (γPKC) gene have been found different SCA14 families. To elucidate how mutant γPKC causes SCA14, we examined molecular properties of seven (H101Y, G118D, S119P, S119F, Q127R, G128D, and F643L) γPKCs fused with green fluorescent (γPKC-GFP). Wild-type γPKC-GFP was expressed...
The mRNA encoding the cloned substance K receptor was microinjected into Xenopus laevis oocytes. After expression of mRNA, Ca2+ imaged in oocytes with a digital imaging fluorescence microscopy system using Ca2(+)-sensitive dyes fura-2 and fluo-3. Application caused dose-related wave mobilization to spread from focus elevate concentration oocyte. Activation endogenous muscarinic or angiotensin II receptors noninjected evoked similar response. rise induced by due internal independent external...
Taxonomic relationships between various species of Bacillus were studied, using 52 test strains 8 species, by deoxyribonucleic acid (DNA)-DNA hybridization and testing transformability the auxotrophic antibiotic resistance genetic markers. Results for identification conventional taxonomic method described in Bergey's Manual (8th ed., 1974) showed good agreement with DNA-DNA data B. licheniformis (16 strains), pumilus (one strain), coagulans (three megaterium cereus brevis strain)....
Niemann–Pick disease type C (NPC) is a recessive hereditary caused by mutation of the NPC1 or NPC2 gene. It characterized abnormality cellular cholesterol trafficking with severe neuronal and hepatic injury. In this study, we investigated potential glycoprotein nonmetastatic melanoma protein B (GPNMB) to act as biomarker reflecting therapeutic effect 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) in an NPC mouse model. We measured serum, brain, liver expression levels GPNMB, evaluated their...
Niemann-Pick disease type C (NPC) is a fatal neurodegenerative disorder caused by abnormal intracellular cholesterol trafficking. Cyclodextrins (CDs), the most promising therapeutic candidates for NPC, but with concerns about ototoxicity, are cyclic oligosaccharides dual functions of unesterified (UC) shuttle and sink that catalytically enhance bidirectional flux net efflux UC, respectively, between cell membrane extracellular acceptors. However, properties CDs regulate these how they could...
Abstract Several causal missense mutations in protein kinase Cγ (γPKC) gene have been found spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease. We previously demonstrated that mutant γPKC SCA14 is susceptible to two types of aggregation, cytoplasmic dot‐like and perinuclear massive causes cell death Chinese hamster ovary cells. Long‐term time‐lapse imaging revealed firstly accumulated aggregation γPKC‐green fluorescent (GFP) gradually formed aggregations,...