Middle East respiratory syndrome coronavirus (MERS-CoV) with pandemic potential is a major worldwide threat to public health. However, vaccine development for this pathogen lags behind as immunity associated protection currently largely unknown. In study, an immunoinformatics-driven genome-wide screening strategy of targets was performed thoroughly screen the vital and effective dominant immunogens against MERS-CoV. Conservancy population coverage analysis epitopes were done by Immune...

<sec> <title>BACKGROUND</title> Diabetes is prevalent worldwide and a chronic metabolic disease. </sec> <title>OBJECTIVE</title> The study developed an application for the guidance of diabetic foot care to explore impact using on patients' assessment awareness self-care management. <title>METHODS</title> design was observational intervention with single-group pre- post-test wait lists (Introduction Wait List Control). This conducted survey in hospital from June 23, 2023, March 31, 2024,...

Background Vascular cognitive impairment (VCI) is a major cause of in the elderly and co-factor development progression most neurodegenerative diseases. With continuing neuroimaging, multiple markers can be combined to provide richer biological information, but little known about their diagnostic value VCI. Methods A total 83 subjects participated our study, including 32 patients with vascular no dementia (VCIND), 21 (VD), 30 normal controls (NC). We utilized resting-state quantitative...

It is generally believed that RNA virus replicating in the cell cytoplasm would not encode microRNAs (miRNAs) due to nucleus inaccessibility. Recent studies have described cytoplasmic genome-derived miRNAs West Nile (WNV) and Dengue (DENV). However, naturally occurring derived from antigenome of a been described. Hepatitis A (HAV) was served as model investigate whether be processed into or miRNA-like small RNAs upon infection. HAV queried for putative miRNA precursors (pre-miRNA) with VMir...

MicroRNAs (miRNAs), including host miRNAs and viral miRNAs, play vital roles in regulating host-virus interactions. DNA viruses encode that regulate the life cycle. However, it is generally believed cytoplasmic RNA do not owing to inaccessible cellular miRNA processing machinery. Here, we provide a comprehensive genome-wide analysis identification of were derived from hepatitis A virus (HAV; Hu/China/H2/1982), which typical virus. Using deep-sequencing silico approaches, identified 2 novel...

Abstract Long non-coding RNAs (lncRNAs) act as competing endogenous (ceRNAs) to compete with microRNAs (miRNAs) in cancer occurrence and development. However, the differential expression of their ceRNA network during development colon (CC) remains unclear. This study was aimed at comprehensive analysis lncRNAs networks associated CC. Whole transcriptome sequencing performed on colorectal adjacent normal tissues different pathological stages. Forty-nine were differently expressed between CC...

Circular RNAs (circRNAs), identified as a class of widely expressed endogenous regulatory RNAs, are involved in diverse physiological and pathological processes. However, their role viral pathogenesis cellular antiviral response remains unexplored. In this study, potent DNA tumor virus, simian virus 40 (SV40), was used model to investigate the influences on circRNA transcriptome. Using RNA-seq, 15,241 putative circRNAs were de novo from 5,057 parental genes monkey kidney-derived Vero cells....

Dengue is the most common mosquito-borne infection worldwide and a serious threat to global public health. Sporadic dengue virus serotype 2 (DENV-2) imported cases from Myanmar have been documented almost every year in Yunnan Province of China since 2005. However, complete genome sequences DENV-2 isolates are not available. The full-length strain (YNPE2), isolated an case 2013, was identified by next-generation sequencing. extreme ends viral were validated 5′/3′ RACE Sanger Furthermore,...

Dengue is one of the most globally serious vector-borne infectious diseases in tropical and subtropical areas for which there are currently no effective vaccines. The highly conserved flavivirus protein, NS5, an indispensable target CD8+ T-cells, making it ideal vaccine design target. Using Immune Epitope Database (IEDB), T-cell epitopes dengue virus (DENV) NS5 protein were predicted by genotypic frequency HLA-A,-B, and-C alleles Chinese population. Antigenicity scores all analyzed using...

The traditional vaccine adjuvant research is mainly based on the trial and error method, mechanisms underlying immune system stimulation remaining largely unknown. We previously demonstrated that heparan sulfate (HS), a TLR-4 ligand endogenous danger signal, effectively enhanced humoral cellular responses in mice immunized by HBsAg. This study aimed to evaluate whether HS induces better against inactivated Hepatitis A or Rabies Vaccines, respectively, compared with adjuvants (e.g. Alum...

Inducing antigen-specific tolerance is a promising treatment for preventing or reversing Type 1 diabetes (T1D). In contrast to vaccine that induces immune responses against pathogens, tolerogenic can suppress immunity antigens causing diseases by administrating mixture of self-antigens with an adjuvant decreases the strength response. Kynurenine (Kyn) endogenous substance inhibit natural killer cell and T proliferation promote differentiation naïve cells into regulatory (Tregs). this study,...

Dengue virus (DENV) infection caused by international visitors has become a public health concern in China. Although sporadic imported cases of DENV have been documented Yunnan, China since 2000, complete genome sequence dengue serotype 3 (DENV-3) from Laos is still not available. Here, we report the first and genomic characterization DENV-3 strain (YNPE3) isolated patient returned Laos. Viral isolation patient's serum was performed using mosquitoes C6/36 cells. Reverse transcriptase...

This study was designed to explore the characteristics of retinal microangiopathy in patients with cerebral small vessel disease (CSVD) and clarify its interaction risk factors for CSVD.Sixty CSVD 15 healthy individuals were enrolled. Demographic data, factors, medical history recorded, magnetic resonance imaging performed detect analyze two groups. The among microangiopathy, vascular total load compared.(1) Hypertension, standard deviation systolic blood pressure (SBPSD), glucose (SDBG),...

Objective To clarify the relationship between serum neurofilament light chain (NfL) and brain structural changes in patients with white matter hyperintensities (WMH), to explore value of NfL for screening progression prediction WMH. Methods Thirty-four healthy people 117 WMH were collected.WMH categorized into mild group moderate-severe group.Gray volume levels compared normal population, group, analyze pattern gray atrophy trend different severity Results Our study found significant...

Type 1 diabetes (T1D) is an autoimmune disorder characterised by the destruction of insulin-producing beta cells in pancreatic islets, resulting from a breakdown immunological tolerance. Currently, T1D treatment primarily relies on insulin replacement or immunosuppressive therapies. However, these approaches often have significant drawbacks, including adverse effects, high costs, and limited long-term efficacy. Consequently, there pressing need for innovative immunotherapeutic strategies...

Hypoplasia of the transverse sinus (TS) is a common anatomical variation. The aim this study was to investigate effects TS variation (i.e., hypoplasia) and no symmetry) their subgroups on clinical outcomes patients with atherosclerotic anterior circulation cerebral infarction (CI).A total 75 were included in divided into group group. further following subgroups: ipsilateral CI contralateral We retrospectively analyzed correlations endpoint events large variant, variants.We found that...