A5047309689- Bacterial Genetics and Biotechnology
- CAR-T cell therapy research
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Cancer Immunotherapy and Biomarkers
- Computational Drug Discovery Methods
- Gut microbiota and health
- Antimicrobial agents and applications
- Heat shock proteins research
- Protein Structure and Dynamics
- Aldose Reductase and Taurine
- Immune Response and Inflammation
- Enzyme Structure and Function
- Tuberculosis Research and Epidemiology
- Luminescence Properties of Advanced Materials
- Synthesis and biological activity
- Enzyme Production and Characterization
- Vibrio bacteria research studies
- Digital Marketing and Social Media
- Advanced Photocatalysis Techniques
- Mass Spectrometry Techniques and Applications
- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Bone Tissue Engineering Materials
Lanzhou University
2015-2024
Shanghai Jiao Tong University
2023-2024
Jiangsu University of Technology
2010
Certain strains of biocontrol bacterium Pseudomonas fluorescens produce the secondary metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) to antagonize soilborne phytopathogens in rhizosphere. The gene cluster responsible for biosynthesis 2,4-DAPG is named phlACBDEFGH and it still unclear how pathway-specific regulator phlH within this regulates metabolism 2,4-DAPG. Here, we found that PhlH strain 2P24 represses expression phlG encoding hydrolase by binding a sequence motif overlapping with -35...
Blockade of the PD-1/PD-L1 immunologic checkpoint using monoclonal antibodies has provided breakthrough therapies against cancer in recent years. Nevertheless, intrinsic disadvantages therapeutic may limit their applications. Thus, blocking interaction by small molecules be a promising alternative for immunotherapy. We used docking-based virtual screening strategy to rapidly identify new molecular inhibitors targeting PD-L1. demonstrated that molecule compound...
Targeting of the PD-1/PD-L1 immunologic checkpoint is believed to have provided a real breakthrough in field cancer therapy recent years. Due intrinsic limitations antibodies, discovery small-molecule inhibitors blocking interaction has gradually opened valuable new avenues past decades. In an effort discover PD-L1 small molecular inhibitors, we carried out structure-based virtual screening strategy rapidly identify candidate compounds. Ultimately, CBPA was identified as inhibitor with KD...
Summary The mqsRA operon encodes a toxin–antitoxin pair that was characterized to participate in biofilm and persister cell formation Escherichia coli . Notably, the antitoxin MqsA possesses C‐terminal DNA‐binding domain recognizes [5’‐AACCT(N) 2‐4 AGGTT‐3′] motif acts as transcriptional regulator controlling multiple genes including general stress response RpoS. However, it is unknown how circuits of homologues have changed bacteria over evolutionary time. Here, we found mqsA Pseudomonas...
Indole is well known as an interspecies signalling molecule to modulate bacterial physiology; however, it not clear how the indole signal perceived and responded by plant growth promoting rhizobacteria (PGPR) in rhizosphere. Here, we demonstrated that enhanced antibiotic tolerance of Pseudomonas fluorescens 2P24, a PGPR for its biocontrol capacity. Proteomic analysis revealed influenced expression multiple genes including emhABC operon encoding major multidrug efflux pump. The was regulated...
Bacteria develop a variety of extracellular fibrous structures crucial for their survival, such as flagella and pili. In this study, we use cryo-EM to identify protein fibrils surrounding lab-cultured Bacillus amyloiquefaciens discover an unreported fibril species in addition the flagellar fibrils. These previously unknown are composed Vpr, serine peptidase. We find that Vpr assembles into enzymatically active form, potentially representing strategy enriching activities around bacterial...
Multidrug resistance (MDR) efflux pumps are involved in bacterial intrinsic to multiple antimicrobials. Expression of MDR can be either constitutive or transiently induced by various environmental signals, which typically perceived two-component systems (TCSs) and relayed the nucleoid, where gene expression is modulated for niche adaptation. Here, we demonstrate that RstA/RstB, a TCS previously shown control acid-induced biofilm-related genes Escherichia coli, confers antibiotics Pseudomonas...
Given the current epidemic of multidrug-resistant tuberculosis, there is an urgent need to develop new drugs combat drug-resistant tuberculosis. Direct inhibitors InhA target do not require activation and thus can overcome drug resistance caused by mutations in drug-activating enzymes. In this work, binding thermodynamic kinetic information its direct inhibitors, phenoxyphenol derivatives, were explored through multiple computer-aided design (CADD) strategies. The results show that van der...
The family of PhlG proteins catalyses the hydrolysis carbon‐carbon bonds and is widely distributed across diverse bacterial species. Two members have been separately identified as 2,4‐diacetylphloroglucinol (2,4‐ DAPG ) hydrolase phloretin hydrolase; however, extent functional divergence catalytic substrates for most this still unknown. Here, using sequence similarity network gene co‐occurrence analysis, we categorized into several subgroups inferred that from Mycobacterium abscessus...
Aberrant canonical NF-κB signaling has been implicated in diseases, such as autoimmune disorders and cancer. Direct disruption of the interaction NEMO IKKα/β developed a novel way to inhibit overactivation NF-κB. Peptides are potential solution for disrupting protein-protein interactions (PPIs); however, they typically suffer from poor stability
Protein filaments are ubiquitous in nature and have diverse biological functions. Cryo-electron microscopy (cryo-EM) enables the determination of atomic structures, even from native samples, is capable identifying previously unknown filament species through high-resolution cryo-EM maps. In this study, we determine structure an unreported a dataset collected Bacillus amyloiquefaciens biofilms. These composed GerQ, spore coat protein known to be involved germination. GerQ assembles into...
FKBP51 is well-known as a cochaperone of Hsp90 machinery and implicated in many human diseases including stress-related diseases, tau-mediated neurodegeneration cancers, which makes an attractive drug target for the therapy FKBP51-associated diseases. However, it has been reported that only nature product rapamycin, cyclosporine A, FK506 its derivatives exhibit good binding affinities when bound to by now. Given advantages peptide-inhibitors, we designed obtained 20 peptide-inhibitor hits...