A5048052125- MicroRNA in disease regulation
- Circular RNAs in diseases
- Neurological Disease Mechanisms and Treatments
- Extracellular vesicles in disease
- Renal cell carcinoma treatment
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
- Renal and related cancers
- Autophagy in Disease and Therapy
- Alzheimer's disease research and treatments
- Medicinal Plants and Bioactive Compounds
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Shoulder Injury and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- IL-33, ST2, and ILC Pathways
- Tendon Structure and Treatment
- Protein Degradation and Inhibitors
- Electrospun Nanofibers in Biomedical Applications
- Botulinum Toxin and Related Neurological Disorders
- Cryospheric studies and observations
- 3D Printing in Biomedical Research
- Hydrogels: synthesis, properties, applications
- Advanced Sensor and Energy Harvesting Materials
- PI3K/AKT/mTOR signaling in cancer
China South Industries Group (China)
2024
Affiliated Hospital of Jiangsu University
2017-2024
Jiangsu University
2017-2024
The Wallace H. Coulter Department of Biomedical Engineering
2024
Guizhou University
2024
Fujian Normal University
2023-2024
Qilu Hospital of Shandong University
2023
Ministry of Education of the People's Republic of China
2022-2023
Northwest University
2022-2023
Nantong University
2010-2022
Exosomes are critically involved in tumor growth, metastasis, and therapy resistance. have the potential to be utilized as cancer biomarkers. In this study, we aimed explore roles clinical values of exosomal miRNAs gastric cancer. We found that concentration exosomes was significantly higher serum patients culture supernatants cells than healthy volunteers mucosa epithelial cells. particular, miR‐423‐5p elevated patients, level remarkably correlated with lymph node metastasis. High...
Exosomes are critically involved in cancer development and progression. The exosomal contents have been suggested as ideal biomarkers. In this study, we investigated the expression of proteins serum gastric patients their roles cancer.The proteomic profile exosomes from was detected by using LC-MS/MS. TRIM3 healthy controls assessed ELISA western blot. Immunohistochemistry used to detect tissues matching adjacent tissues. growth migration abilities cells with overexpression or knockdown...
The deficiency or mutation of p53 has been linked to several types cancers. mesenchymal stem cell (MSC) is an important component in the tumor microenvironment, and exosomes secreted by MSCs can transfer bioactive molecules, including proteins nucleic acid, other cells microenvironment influence progress a tumor. However, whether state impact molecule secretion promote cancer progression regulatory mechanism remains elusive. Our study aimed investigate regulation ubiquitin protein ligase E3...
Abstract Tobacco smoke (TS) is the most important single risk factor for bladder cancer. Epithelial–mesenchymal transition (EMT) a transdifferentiation process, involved in initiation of TS-related Cancer stem cells (CSCs) have an essential role progression many tumors including However, molecular mechanisms TS exposure induced urocystic EMT and acquisition CSCs properties remains undefined. Wnt/ β -catenin pathway critical maintenance CSCs. The aim our present study was to investigate...
Osteoarthritis (OA) constitutes a widespread degenerative joint disease predominantly affecting the elderly, leading to disability. There is still lack of biomarkers for OA, so it cannot be intervened in time. OA were identified from human cartilage datasets using LASSO and SVM-RFE, followed by ROC analysis. LOXL1 was prioritized further research due its high expression robust predictive performance. Anterior cruciate ligament transection (ACLT) surgery-induced rats used explore correlation...
Here, we demonstrated that DNA-PKcs is over-expressed in multiple human renal cell carcinoma (RCC) tissues and primary/established RCCs. Pharmacological or genetic inhibition of suppressed proliferation RCC cells. was the complex mTOR SIN1, mediating mTORC2 activation HIF-2α expression Inhibiting silencing AKT Ser-473 phosphorylation expression. In vivo, knockdown oral administration inhibitor NU-7441 inhibited phosphorylation, 786-0 xenograft growth nude mice. We showed miRNA-101 level...
Mutations in optineurin (OPTN) are associated with several human disorders including amyotrophic lateral sclerosis (ALS) and primary open-angle glaucoma (POAG). OPTN is known to be a multifunctional autophagy receptor that plays important roles NF-κB signaling, vesicle trafficking, maintenance of the Golgi apparatus autophagy. Given loss neurons an abnormal aggregation disease proteins two key features neurodegenerative diseases, protein quality control systems considered tightly...
Abstract Introduction Rheumatoid arthritis (RA) is a chronic inflammatory disease involving variety of immune cells, including adaptive T and B cells innate lymphoid (ILCs). Understanding the pathogenic role these in RA provides new insights into intervention treatment RA. Methods A total 86 patients with (RA group) 50 healthy controls (HC) were included study. The CD4 + , CD19 B, NK, Th17, Treg, ILCs, their subsets (i.e., ILC1s, ILC2s, ILC3s) characterized peripheral blood mononuclear by...
miRNAs play a crucial part in multiple biological processes of cell proliferation, migration, apoptosis, and chemoresistance. In cancer, can be divided into oncogenes or tumor suppressors on the basis their functions carcinogenic process. The purpose this study was to explore roles clinical diagnostic value miR-370-3p breast cancer. Our results demonstrated that significantly promoted metastasis, stemness cancer vitro vivo. particular, data revealed high expression serum exosomal from...
Multidrug resistance (MDR) has become one of the most intractable problems in clinics as it would cause failure chemotherapy. In this study, we demonstrated that a nanoscale self-assembled nanomedicine, which almost consisted pure chemo-drug, could efficiently overcome MDR. Celastrol (CST) was directly assembled into discrete nanomedicine by precipitation, and then CST nanoparticles (CNPs) inhibited drug efflux pumps activating HSF-1 expression promoting translocation nucleus to suppress Pgp...
The expression and biological function of Grb2-associated binding 2 (Gab2) in renal cell carcinoma (RCC) cells was tested here. We showed that Gab2 significantly elevated human RCC tissues cells. It correlated with over-activation Akt downregulation microRNA-302c-3p ("miR-302c-3p"), a putative Gab2-targeting microRNA. Knockdown inhibited activation 786-O proliferation. Reversely, forced over-expression led to hyper-activation facilitate Exogenous miR-302c caused downregulation, inhibition...
Mammalian target of rapamycin (mTOR)in renal cell carcinoma (RCC) represents a valuable oncotarget for treatment. We here tested the potential anti-RCC activity by novel mTOR kinase inhibitor WYE-687in vitro and in vivo.WYE-687 was cytotoxic anti-proliferative to established RCC lines (786-O A498) primary human cells. Yet, it non-cytotoxic toHK-2 tubular epithelial cells.WYE-687 provoked caspase-dependent apoptosis At molecular level, WYE-687 almost completely blocked mTORC1 (p-S6K1 p-S6)...
Bromodomain-containing protein 4 (BRD4) and PI3K-AKT are both important for renal cell carcinoma (RCC) development progression. SF2523 is a BRD4 dual inhibitor. The present study demonstrated that was cytotoxic anti-proliferative to established RCC lines (786-O A498) primary human cells. induced activation of caspase apoptosis in Further, disrupted cycle progression inhibited migration vitro. At the signaling level, in-activated PI3K-AKT-mTOR, downregulated BRD4-dependent proteins, Bcl-2...
Injectable hybrid hydrogels were cross-linked multi-arm amino-terminated poly(ethylene glycol) (PEG) with aldehyde hyaluronic acid (HA) upon mixing, without the addition of a chemical crosslinking agent. The gelation is attributed to Schiff-base reaction between amino and groups PEG oxidized HA. In current work, 4-arm 8-arm crosslinked HA characterized. vitro time, morphologies, swelling ratio, weight loss compressive modulus PEG/HA in phosphate buffered saline at 37°C studied. Results...
Helminths and their products can regulate immune response offer new strategies to control alleviate inflammation, including asthma. We previously found that a peptide named as SJMHE1 from Schistosoma japonicum suppress asthma in mice. This study mainly investigated the molecular mechanism of inhibiting inflammation.SJMHE1 was administered mice with OVA-induced via subcutaneous injection, its effects were detected by testing airway inflammation The Th cell distribution analyzed flow...
Cerebral neuroinflammation models were established by injecting 10 μg lipopolysaccharide into the hippocampus of male Sprague-Dawley rats. The rats treated with an intraperitoneal injection 120, 90, or 60 mg/kg oxymatrine daily for three days prior to injection. Twenty-four hours after model induction, was analyzed real-time quantitative PCR, and cerebral cortex enzyme-linked immunosorbent assay western blot assay. results PCR showed that secretion mRNA expression pro-inflammatory cytokines...