A5107117996- Natural product bioactivities and synthesis
- Phytochemicals and Medicinal Plants
- Phytochemical Studies and Bioactivities
- Phytochemical compounds biological activities
- Plant tissue culture and regeneration
- Essential Oils and Antimicrobial Activity
- Prenatal Screening and Diagnostics
- Phytochemistry and Biological Activities
- Medicinal Plants and Neuroprotection
- Plant-based Medicinal Research
- Plant biochemistry and biosynthesis
- Pharmacological Effects of Natural Compounds
- Medicinal Plants and Bioactive Compounds
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Insect Pest Control Strategies
- Natural Antidiabetic Agents Studies
- Hepatitis B Virus Studies
- Natural Compounds in Disease Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Traditional Chinese Medicine Analysis
- Antioxidant Activity and Oxidative Stress
- Nuts composition and effects
- Plant-Derived Bioactive Compounds
- Ginseng Biological Effects and Applications
Heilongjiang University of Chinese Medicine
2016-2024
Kunming University of Science and Technology
2024
Innovation Team (China)
2024
First Affiliated Hospital of Heilongjiang University of Chinese Medicine
2023
Third Hospital of Hebei Medical University
2020
Hebei Medical University
2020
Ningxia University
2017
Zhengzhou University
2015
Chinese Academy of Forestry
2014
Research Institute of Tropical Forestry
2014
Abstract In this study, we observed loss of heterozygosity (LOH) in human chromosomal fragment 6q25.1 sporadic lung cancer patients. LOH was 65% the 26 tumors examined and narrowed down to a 2.2-Mb region. Single-nucleotide polymorphism (SNP) analysis genes located within region identified candidate gene, termed p34. This also designated as ZC3H12D, C6orf95, FLJ46041, or dJ281H8.1, carries an A/G nonsynonymous SNP at codon 106, which alters amino acid from lysine arginine. Nearly 73%...
A new sesquiterpenoid glycoside, consisting of one vetispirane-type, dmetelisproside (1), together with two known megastigmane sesquiterpenoids, citroside (2) and staphylionoside D (3) were isolated from the leaves Datura metel L. The structures determined by analysis their MS NMR data as well comparison literature values. All isolates evaluated for cytotoxicity toward SGC-7901, Hepg2, HeLa, MCF-7, MDA-MB-231 cancer cell lines, anti-inflammatory activity against nitric oxide (NO) in RAW...
Four new steroid saponins 1–4 were isolated from the rhizomes of Anemarrhena asphodeloides (Asparagaceae), as well four known saponins: anemarsaponin B (5) timosaponin D (6), E1 (7) II (8). Their structures established through UV and NMR MS data. All compounds evaluated for cytotoxicity against HepG2 SGC7901 human cancer lines. Compounds 3 7 displayed medium antiproliferative activities on cells, with IC50 values 43.90 57.90 μM, respectively.
Phytochemical investigation of the roots Solanum melongena L. resulted in isolation ten terpenes and sixteen lignans, including a new triterpene saponin, officinoterpenoside E (1) twenty-five known compounds (2-26). All were firstly isolated from S. except 2, 13, 21, 22. The structures these determined by 1 D 2 NMR spectra referring to literatures, together with high-resolution mass spectrometric analysis. evaluated for cytotoxicity against three cancer cell lines (HepG2, Hela, MCF-7) vitro....
Abstract Fifteen constituents, including one new lignan (schisandroside E) and terpenoid (schisandenoid A) as well nine known lignans four terpenoids, were isolated from Schisandra chinensis leaves. The structures of schisandroside E schisandenoid A established by entirely meticulous spectroscopic analysis (NMR, MS, CD, IR UV). All compounds tested for cytotoxicity against MGC‐803, Caco‐2 Ishikawa cell lines. Some showed strong these three cancer lines with IC 50 <1 μ m .
Epilepsy (EP) is one of the most common neurological diseases in world. Anemarrhena asphodeloides Bunge. (AA), as a typical heat-cleaning medicine, has been proven to possess antiepileptic effect clinical and experimental studies. steroidal saponins (AAS) are main components. However, therapeutic effects underlying mechanisms AAS against EP not fully elucidated. In this study, 63 were discovered by UPLC-Q-TOF/MS analysis. Pharmacological behavioral analysis demonstrated that could...
We explored the application of single nucleotide polymorphism microarray (SNP array) in molecular karyotype analysis for early spontaneous abortion detection assisted reproductive technology (ART). SNP array was performed 81 cases. Of cases, 16 experienced natural conception (NC) and 65 were pregnant by ART. 4 underwent artificial insemination (AI), 32 fresh vitro fertilization-embryo transfer (IVF-ET), 9 intracytoplasmic sperm injection (ICSI), 20 thawed embryo transfer. In cases examined...
Phenylpropionamides in the seed of Cannabis sativa L. (PHS) have a protective effect on neuroinflammation and antioxidant activity. In this study, UHPLC-Orbitrap-fusion-TMS-based metabolomics approach was used to analyze serum samples identify potential biomarkers Streptozotocin (STZ) induced Alzheimer's disease (AD) rats. The results revealed that primary bile acid biosynthesis taurine hypotaurine metabolism were significantly correlated with STZ-induced AD addition, key enzymes these two...
Withanolides from six parts (flower, leaf, stem, root, seed, and peel) of Datura metel L. (D L.) obtained ten production areas in China were identified quantified by UPLC-MS/MS. A total 85 withanolides characterized for the first time using UPLC-Q-TOF-MS/MS system. Additionally, a simultaneous, rapid accurate measurement method was developed determination 22 bioactive with UPLC-Q-TRAP-MS/MS The results show withanolide content is highest leaves (155640.0 ng/g) lowest roots (14839.8 ng/g)....
Five new oleanane-type triterpenoid saponins (1-5), together with 24 known (6-29) were isolated from the fruit of Acanthopanax senticosus. Their structures determined by extensive spectroscopic analysis, including 1D, 2D nuclear magnetic resonance (NMR), and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), in combination chemical methods (acid hydrolysis). The neuroinflammation model was established lipopolysaccharide (LPS)-induced BV2 microglia, neuroprotective effects...
Seven new glycosides (1-7) and seventeen known analogues (8-24) were isolated from the leaves of Datura metel L. The structures these compounds all elucidated by detailed spectroscopic analyses comparison with literature values. All isolates evaluated for cytotoxicity against Hela, MGC-803, Ishikawa cell lines 6, 7, 14-17 24 exhibited different degrees antiproliferative effects.
A new ent-kaurane diterpenoid, named kaurane daturoside (1), was isolated from the 70%-EtOH extract of dried pericarps Datura metel L., along with six known terpenoids, 16α,17-dihydroxy-ent-kauran-19-diglycoside (2), cyclosieversioside F (3), astragaloside II (4), ginsenoside Rg1 (5), astrojanoside (6), celerioside E (7). The structures were elucidated by means spectroscopic analyses, and compounds 2, 3, 7 separated Solanaceae for first time. Meanwhile, among isolates, 2 5 exhibited...
Fifteen phenylpropanoids were isolated from the ethanol extract of roots Solanum melongena L., including a new compound, melongenapanoid A (1), together with fourteen known compounds (2-15). Their chemical structures elucidated by 1D and 2D NMR HR-MS data according to those values literatures. The (2-15) all firstly this plant. While, isolates evaluated for inhibitory activities on nitric oxide (NO) production induced lipopolysaccharide (LPS) in RAW 264.7 cell line. Compounds 2, 4 5 showed...
Congenital talipes equinovarus (CTEV) is a common birth defect with an unclear genetic pathogenesis that results from both and environmental factors. The present study aimed to identify novel variants in patients CTEV using whole‑exome sequencing (WES) investigate the factors responsible for development of CTEV.A cohort nine neonates/infants suspected was recruited. Subsequently, sequential tests, including chromosome karyotyping WES, were performed each participants. Familial validation...
Human papillomavirus (HPV) is associated with cervical cancer. In this study, we developed a high-throughput microwell-plate hybrid capture (MPHC) method for epidemiological studies of high-risk HPV (HRHPV). The results 1238 specimens from female outpatients showed concordance rate 94.3 % between the MPHC and Hybrid Capture II assay. assay an average HRHPV 29.3 populations in populous cities China. established could sensitively specifically detect 13 types suitable large-scale screening,...
Two new terpenoids (1 and 2), arenterpenoid D (1) pinnasesquiterpene A (2), along with 16 phenylpropanoids (3-18) 8 known (19-26) were isolated from 70% EtOH extract of the Arenga pinnata (Wurmb) Merr. fruits. Their structures elucidated by spectroscopic methods including HR-ESI-MS, 1D, 2D NMR. The absolute configuration was defined X-ray crystallographic analysis. Furthermore, we evaluated anti-inflammatory activity all compounds, outcomes showed that 2 21 exposed moderate suppressive...
Two new acyclic sesquiterpenoids (1-2) and fourteen known monocyclic monoterpenoids (3-16) were isolated from the aerial parts of Clematis chinensis Osbeck. All compounds C. for first time. The structures all characterized by spectroscopic methods (1 D, 2 D NMR HRESIMS). In-vitro cytotoxic activity against two human cancer cell lines (MGC-803 Ishikawa) evaluated CCK-8 assay.
Establishing the genetic transformation system of medicinal plant is important to study their functional genes. Based on established regeneration Sophra alopecuroides, 6 factors were optimized, that was concentration Agrobacterium tumefaciens, infection time, co-cultivation time agrobacterium tumefaciensand S.alopecuroides callus, preculture adding method ofacetosyringone (AS) and AS, respectively. The results showed a maximum efficiency 83.33% achieved with 15d-precultured which infected by...