A5110017465- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Vectors
- Cancer Diagnosis and Treatment
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Oral microbiology and periodontitis research
- Aquaculture disease management and microbiota
- Colorectal Cancer Surgical Treatments
- Multiple and Secondary Primary Cancers
- Receptor Mechanisms and Signaling
- Retinal Development and Disorders
- Chemical synthesis and alkaloids
- Genetic factors in colorectal cancer
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Synthetic Organic Chemistry Methods
- Diabetes Treatment and Management
- Herpesvirus Infections and Treatments
- Axial and Atropisomeric Chirality Synthesis
- Colorectal and Anal Carcinomas
Oregon Health & Science University
2013-2024
Osaka University
2024
Aichi Gakuin University
2014-2020
NTT WEST Osaka Hospital
2017-2018
University of Pittsburgh
2010-2011
Hiroshima University
2010
Saitama Medical University
2009
Nagasaki University
2007-2008
Kyoto Pharmaceutical University
1995-1998
Kyoto University
1994-1998
Abstract Adeno-associated virus (AAV) capsid engineering is an emerging approach to advance gene therapy. However, a systematic analysis on how each amino acid contributes multiple functions remains challenging. Here we show proof-of-principle and successful application of novel approach, termed AAV Barcode-Seq, that allows us characterize phenotypes hundreds different strains in high-throughput manner therefore overcomes technical difficulties the analysis. In this generate DNA...
Adeno-associated virus (AAV) vectors have made great progress in their use for gene therapy; however, fundamental aspects of AAV's capsid assembly remain poorly characterized. In this regard, the discovery assembly-activating protein (AAP) sheds new light on crucial part AAV biology and vector production. Previous studies shown that AAP is essential assembly; how its mechanistic roles might differ among serotypes remains uncharacterized. Here, we show biological properties AAPs processes are...
A relationship between periodontal disease and atherosclerosis has been suggested by epidemiological studies. Ligature-induced experimental periodontitis is an adequate model for clinical periodontitis, which starts from plaque accumulation, followed inflammation in the tissue. Here we have demonstrated using a ligature-induced that activates monocytes/macrophages, subsequently circulate blood adhere to vascular endothelial cells without altering serum TNF-α concentration. Adherent...
Calmodulin-dependent cyclic nucleotide phosphodiesterase from bovine brain is found to be composed of two distinct subunits, 60,000-and 63,000-dalton polypeptides.Peptide mapping the subunits by partial proteolysis demonstrated that 60-kDa polypeptide not derived 63-kDa species.The interaction enzyme with three monoclonal antibodies, A6, C 1, and A2, analysis immunocomplexes sucrose density gradient centrifugation revealed calmodulin-dependent exists in different forms, Le. (a) homodimer...
Recombinant adeno-associated virus serotype 9 (rAAV9) vectors show robust in vivo transduction by a systemic approach. It has been proposed that rAAV9 enhanced ability to cross the vascular endothelial barriers. However, scientific basis of administration and its mechanisms have not fully established. Here, we indirect evidence suggesting capillary walls still remain as significant barrier cardiac but so hepatic mice, distinctively delayed blood clearance plays an important role overcoming...
ABSTRACT Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2) is a nucleolar-localizing that plays critical role in transporting the viral capsid VP3 to nucleolus for assembly. Here, we identify and characterize AAV2 AAP (AAP2) nuclear (NLS) nucleolar (NoLS) localization signals near carboxy-terminal region AAP2 (amino acid positions 144 184) (AAP2 144–184 ). This contains five basic-amino-acid-rich (BR) clusters, KSKRSRR (AAP2BR1), RRR (AAP2BR2), RFR (AAP2BR3),...
Abstract Aims/Introduction The involvement of glucose‐dependent insulinotropic polypeptide ( GIP ) on inflammation was explored in atherosclerosis and adipose tissue. Periodontal disease is a chronic inflammatory disease, considered one the diabetic complications. In present study, to examine effect periodontitis, we induced experimental periodontitis receptor‐knockout mice GIPRKO ). We also investigated anti‐inflammatory culture system. Materials Methods Experimental by ligature wire C57 BL...
AAV vectors show promise for gene therapy; however, kidney transfer remains challenging. Here we conduct a barcode-seq-based comparison of 47 capsids administered through different routes in mice, followed by individual validation. We find that local delivery AAV-KP1, but not AAV9, via the renal vein or pelvis effectively transduces proximal tubules with minimal off-target liver transduction, while systemic enhances tubule and podocyte transduction chronic disease. demonstrate these...
Abstract Es wird eine Verallgemeinerung der in I und II vorgeschlagenen Analysenmethode polarographischen Stromspannungskurven beschrieben. Diese verallgemeinerte Methode kann auf Systeme mit folgendem Reaktionsmechanismus angewandt werden: Eine ganze Reihe verschiedenen Komplexe existiert Lösung nebeneinander die sukzessiven Dissoziations‐ Assoziationsreaktionen zwischen Komplexen stehen auch bei Stromfluß im praktisch eingestellten Gleichgewicht. Alle vorhandenen können sich eigenen...
Abstract Despite recent remarkable advancements in adeno-associated virus (AAV) vector technologies, effective gene delivery to the kidney remains a significant challenge. Here we show that AAV transduction proximal tubules and podocytes, crucial targets for renal therapy, can be enhanced remarkably through meticulous selection of both capsids route administration, tailored condition kidney. In this study, performed side-by-side comparison 47 using Barcode-Seq identified six capsids,...
Directed evolution through genetic engineering of viral capsids followed by selection has emerged as a powerful means to create novel recombinant adeno-associated virus (rAAV) vectors with desired tropism and enhanced properties. One the most effective approaches uses rAAV-based random peptide display libraries. Here we report system based on an infection-defective rAAV1.9-3 platform for display, show that biopanning libraries in vitro effectively identifies peptides restore enhance rAAV...
Periodontitis is one of the diabetic complications due to its high morbidity and severity in patients with diabetes. The prevention periodontitis especially important because relationship between diabetes bidirectional. Here, we evaluated impacts glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide on amelioration periodontitis. Five-wk-old Male Sprague–Dawley (SD) rats ( <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mi>n</a:mi> <a:mo>=</a:mo> <a:mn>30</a:mn>...
Overlapping open reading frames (ORFs) in viral genomes undergo co-evolution; however, how individual amino acids coded by overlapping ORFs are structurally, functionally, and co-evolutionarily constrained remains difficult to address conventional homologous sequence alignment approaches. We report here a new experimental computational evolution-based methodology this question its preliminary application elucidating mode of co-evolution the frame-shifted adeno-associated virus (AAV) serotype...
Abstract Background Betanodaviruses, members of the family Nodaviridae , have bipartite, positive-sense RNA genomes and are causal agents viral nervous necrosis in many marine fish species. Recently, viruses were shown to infect a few freshwater species including model medaka ( Oryzias latipes ). Although virological study using cultured cells would provide lot insight into virus-fish interactions molecular aspects, no such yet been tested for virus susceptibility. Results We ten cell lines...
The association between diabetes and periodontal disease is considered to be bidirectional. However, there still controversy surrounding the relationship type 1 diabetes. We investigated whether insulin improves periodontitis without any local treatments for under conditions using ligature-induced experimental model.Type diabetic rats were induced by streptozotocin injection. Experimental was ligature in normal rats. Half of treated with insulin. Two weeks after ligature, evaluated.Insulin...