Cardiomyocyte apoptosis is a major event in the pathogenesis of diabetic cardiomyopathy. Currently, no single effective treatment for cardiomyopathy exists. The present study investigated whether advanced oxidative protein products (AOPPs) have detrimental role survival cardiomyocytes and if glucagon‑like peptide‑1 (GLP‑1) exerts cardioprotective effect under these circumstances. also aimed to determine underlying mechanisms. H9c2 cells were exposed increasing concentrations AOPPs presence...

Abstract Background Gastric cancer (GC) is a common-sighted which hard to cure over the world. Substantial researches revealed that long non-coding RNAs (lncRNAs) were fundamental regulators in process of cancers. Nevertheless, biological function LINC00511 and how was involved regulatory system GC remained unclear. Methods RIP assays luciferase reporter performed illustrate combination between miR-625-5p. Loss-of-function applied for identifying GC. Results In our study, discovered...

Irinotecan, an analog of camptothecin, which is inhibitor topoisomerase I, currently used in the treatment metastatic colorectal cancer. Camptothecin derivatives have been demonstrated to exert radiosensitizing effects on several types cancer cells. However, date, at least best our knowledge, few studies examined these cell lines. In present study, we irinotecan p53-mutant lines, HT29 and SW620, explored potential underlying mechanisms. Drug cytotoxicity tests revealed that 24 h half-maximal...

// Mingzhi Song 1, 2 , Yong Wang 3 Zhen Zhang * Shouyu 1 Department of Orthopaedics, The First Affiliated Hospital Dalian Medical University, 116011, Dalian, Liaoning, People's Republic China Third 116200, Jinpu New Area, Central Shenyang College, 110024, Shenyang, and contributed equally to this manuscript Correspondence to: Zhang, email: doctorzz@126.com Wang, dmuwsy@sina.com Keywords: osteosarcoma; PSMC2; carcinogenesis; oncogenes Received: January 01, 2016 Accepted: November 04,...

Advanced glycation end product (AGE)-induced vascular smooth muscle cell (VSMC) proliferation is vital to the progression of diabetic vasculopathy. A grape seed procyanidin extract has been reported possess anti-oxidative and anti-inflammatory properties display a significant cardiovascular protective effect, but little know about underlying mechanism. The objective this present study was determine whether GSPB2 (grape B2), which dimeric more biologically active, could inhibit AGE-induced...

It is well known that the activation of Aurora A/B (Aur A/B) or inactivation BRCA1/2 induces tumor formation. Others and we have reported mutual suppression between Aur may manipulate cancer cell growth tumorigenesis, however, interactive regulation mechanism these molecules are still elusive. In this study, by consecutive silencing or/and with specific shRNAs, showed that, in BRCA2-deficient pancreatic line Capan-1 ovarian OVCA433, inversely regulated expression each other likely through...

The peroxisome proliferator-activated receptor γ (PPARγ) is emerging as an important regulator in various metabolic processes of cancer. Genistein, a major isoflavonoid isolated from dietary soybean, possesses wide variety biological activities, particularly, cancer prevention. However, the mechanisms by which genistein elicits its growth inhibiting effects osteosarcoma (OS) MG-63 cells have not been extensively elucidated. were treated for 2 days with concentrations and/or GW9662 (a...

Colorectal cancer is the third most frequently diagnosed and combination of radiation with capecitabine has been shown to achieve only 15% 25% pathologic complete response. This study aimed investigate effect MLN4924, a potent small molecule inhibitor SKP1-Cullin-F-box proteins E3 ubiquitin ligases, as novel radiosensitizing agent in colorectal cells. Indeed, we found that MLN4924 effectively sensitized cells sensitivity-enhancement ratio 1.61 for HT-29 1.35 HCT-116 Mechanistically,...

Conversion from fibroblasts to neurons has recently been successfully induced. However, the underlying mechanisms are poorly understood. Here, we find that depletion of p53 alone converts into all three major neural lineages. The induced neuronal cells express multiple neuron-specific proteins and generate action potentials transmitter-receptor-mediated currents. Surprisingly, does not affect well-known tumorigenic target, p21. Instead, knockdown upregulates neurogenic transcription factors,...

Metal-containing compounds have been extensively studied for many years as potent proteasome inhibitors. The 20S proteasome, the main component of ubiquitin pathway, is one excellent targets in anticancer drug development. We recently reported that several copper complexes were able to inhibit cancer-special and induce cell death human cancer cells. However, involved molecular mechanism not known yet. therefore synthesized three investigated their abilities on inhibiting activity inducting...

BACKGROUND:The aim of this study was to investigate the effects and mechanisms long noncoding (lnc) RNA FOXD2-AS1 in hepatocellular carcinoma development. MATERIAL AND METHODS:Collecting 3 pairs adjacent tissue analysis by gene chip. Evaluating expression situ hybridization assay. Bel-7402 biological activity vitro Cell Counting Kit-8, flow cytometry, Transwell wound healing assay correlation between miR-185 dual-luciferase reporter The relative proteins expressions were evaluated western...

Thioredoxin (Trx) is a pro-oncogenic molecule that underlies tumor initiation, progression and chemo-resistance. PX-12, Trx inhibitor, has been used to treat certain tumors. Currently, factors predicting sensitivity PX-12 are unclear. Given hydrogen sulfide (H2S), gaseous bio-mediator, promotes activity, we speculated it might affect response PX-12. Here, tested this possibility. Exposure of several different types cells caused cell death, which was reversely correlated with the levels...

The antipsychotic drug pimozide has been found to exhibit anticancer effects. Previously, it was demonstrated that inhibits hepatocellular carcinoma (HCC) cell growth, but its pharmacodynamic characteristics remain unclear. aim of the present study investigate reversibility and mechanism ability inhibit proliferation in liver cancer. Cell viability determined by Counting Kit‑8 colony formation assay. cycle distribution analyzed flow cytometry with Ki‑67 PI staining. ROS production HCC cells...

Cefepime (FEP), which is a member of the fourth-generation cephalosporin class, has been extensively studied as biochemical and antimicrobial reagent in recent years. Manganese (Mn) important physiological processes many living organisms, it also high expressed some tumor tissues. In present study, we aimed to investigate proteasome-inhibitory anti-proliferative properties 8 metal complexes (FEP‑Cu, FEP-Zn, FEP-Co, FEP-Ni, FEP-Cd, FEP-Cr, FEP-Fe, FEP-Mn) MDA-MB‑231 human breast cancer cells....

Background: Colorectal cancer (CRC) is one of the most common aggressive neoplasms worldwide. Circular RNAs (circRNAs) have been involved in biological process CRC. This study aimed to explore effects circ_0005927 on CRC progression and underneath mechanism. Materials Methods: The expression circ_0005927, microRNA-942-5p (miR-942-5p) basic leucine zipper ATF-like transcription factor 2 (BATF2) was detected by quantitative real time polymerase chain reaction (qRT-PCR). protein BATF2...

Glucagon-like peptide-1 (GLP-1) and its analog exendin (EX)-4 have been considered to promote β-cell growth expansion. In the present, study we investigated effect of GLP-1 on proliferative activity cell cycle regulation in pancreatic insulin-secreting line, INS-1, treated with intermittent high glucose. INS-1 cells were normal glucose (5.5 mmol/l), constant (30 mmol/l) (rotation/24 h 5.5 or 30 presence absence (100 nmol/l) for seven days. Proliferative activity, expression cyclin D1, p21,...

Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 TC still unclear, so it remains be explored. aim research investigate and regulatory mechanism TC.TMPO-AS1 TMPO expression tumors cells was detected by TCGA database QRT-PCR assay respectively. CCK-8, EDU, TUNEL western blot assays were conducted identify biological functions TC. Luciferase reporter RNA...

<title>Abstract</title> Migrasomes are newly discovered organelles with demonstrated functions in organ morphogenesis and angiogenesis. However, the effect of migrasomes tissue repair remains unreported. Our transmission electron microscopy findings revealed that were directly connected retraction fibers could release their contents into surroundings human rat skins oral mucosae. Multiplex immunofluorescence staining results these originated from fibroblasts. Live-cell imaging mucosal...