A5048281975- Congenital Heart Disease Studies
- Mechanical Circulatory Support Devices
- Colorectal Cancer Treatments and Studies
- Heart Failure Treatment and Management
- Sepsis Diagnosis and Treatment
- Skin and Cellular Biology Research
- Cardiac Arrest and Resuscitation
- Colorectal and Anal Carcinomas
- Cellular Mechanics and Interactions
- Cardiac, Anesthesia and Surgical Outcomes
- Respiratory Support and Mechanisms
- Cancer therapeutics and mechanisms
- Neonatal Respiratory Health Research
- Hair Growth and Disorders
- Barrier Structure and Function Studies
- Tissue Engineering and Regenerative Medicine
- Composite Material Mechanics
- Health and Medical Research Impacts
- Lung Cancer Treatments and Mutations
- Nanoplatforms for cancer theranostics
- Artificial Intelligence in Healthcare and Education
- Polymer crystallization and properties
- Hepatocellular Carcinoma Treatment and Prognosis
- Nanoparticle-Based Drug Delivery
- Neonatal and Maternal Infections
Duke University
2013-2025
Duke Medical Center
2012-2024
Duke University Hospital
2012-2023
Carolina Institute for NanoMedicine
2016-2018
University of North Carolina at Chapel Hill
2014-2018
The epidermis is a multilayered epithelium that requires asymmetric divisions for stratification. A conserved cortical protein complex, including LGN, nuclear mitotic apparatus (NuMA), and dynein/dynactin, plays key role in establishing proper spindle orientation during divisions. requirements the recruitment of these proteins, however, remain unclear. In this work, we show NuMA required to recruit dynactin cell cortex keratinocytes. NuMA's LGN; LGN interactions are not sufficient...
Novel agents are needed to improve chemoradiotherapy for locally advanced rectal cancer. In this study, we assessed the ability of CRLX101, an investigational nanoparticle-drug conjugate containing payload camptothecin (CPT), therapeutic responses as compared with standard chemotherapy. CRLX101 was evaluated a radiosensitizer in colorectal cancer cell lines and murine xenograft models. potent CPT vitro its radiosensitize cells. Evaluations vivo demonstrated that addition significantly...
During differentiation, many cells reorganize their microtubule cytoskeleton into noncentrosomal arrays. Although these microtubules are likely organized to meet the physiological roles of tissues, functions in most cell types remain unexplored. In epidermis, differentiation induces reorganization cell-cell junctions a desmosome-dependent manner. Here, we recapitulate cultured epidermal cells. Using this assay, show that cortical recruit myosin II cortex order engage adherens junctions,...
To explore threats and opportunities to chart a path for safely navigating the rapid changes that generative artificial intelligence (AI) will bring clinical research, Duke Clinical Research Institute convened multidisciplinary think tank in January 2024. Leading experts from academia, industry, nonprofits, government agencies highlighted potential of AI automation documentation, strengthening participant community engagement, improvement trial accuracy efficiency. Challenges include...
Many tissues in our body experience mechanical stresses caused by both internal and external forces. The skin, for example, must tolerate diverse insults. In this paper, we report a role β-catenin providing stability to epithelia under stress. Loss of during epidermal development perinatal lethality. Mutant embryos up-regulated stress responses at sites active morphogenesis, which became more widespread after the associated with birth. addition, selective loss tight junctions occurred focal...
Proper development and tissue maintenance requires cell-cell adhesion structures, which serve diverse crucial roles in morphogenesis. Epithelial tissues have three main types of junctions: tight junctions, play a major role barrier formation, adherens junctions desmosomes, provide mechanical stability organize the underlying cytoskeleton. Our current understanding function is hindered by lack tools methods to image mammals. To better understand dynamics we created knock-in ZO-1-GFP mouse...
<b>Background:</b> Infants undergoing cardiac surgery with cardiopulmonary bypass (CPB) frequently receive intra-operative methylprednisolone (MP) to suppress CPB-related inflammation; however, the optimal dosing strategy and efficacy of MP remain unclear. <b>Methods:</b> We retrospectively analyzed all infants under 90 days-old who received for CPB from 2014–2017 at our institution. combined real-world data electronic health record (EHR) two previously developed population...
The difference in the incidence of early-onset sepsis caused by group B streptococcus among term neonates whose mothers received first-line vs second-line intrapartum prophylaxis is poorly described.
Abstract Cardiac surgery‐associated acute kidney injury (CS‐AKI) occurs in approximately 65% of neonates undergoing cardiac surgery on cardiopulmonary bypass and contributes to morbidity mortality. Caffeine may reduce CS‐AKI by counteracting adenosine receptor upregulation after bypass, but pharmacokinetics (PK) this population are unknown. The goal our analysis is address knowledge gaps age‐, disease‐, bypass‐related effects caffeine disposition explore preliminary associations between...
High-quality, efficient, pharmacokinetic (PK), pharmacodynamic (PD), and safety studies in children are needed. Point-of-care trials adults have facilitated clinical trial participation for patients providers, minimized the disruption of workflow, capitalized on routine data collection. The feasibility value point-of-care to study PK/PD unknown, but appear promising. Opportunistic Trial Critically Ill Children with Heart Disease (OPTIC) is a programmatic approach critically ill that seeks...
OBJECTIVES Early warning scores detecting clinical deterioration in pediatric inpatients have wide-ranging performance and use a limited number of features. This study developed machine learning model leveraging multiple static dynamic features from the electronic health record to predict composite outcome unplanned transfer ICU within 24 hours inpatient mortality 48 hospitalized children. METHODS Using retrospective development cohort 17 630 encounters across 10 388 patients, 2 models...
Acyclovir is the first-line therapy for neonatal herpes simplex virus infections. Therapy can mitigate morbidity and mortality but carries a risk toxicity. We aimed to compare acyclovir dosing in intensive care units published recommendations based on population pharmacokinetic (PopPK) analysis.