A5100323015- Radiopharmaceutical Chemistry and Applications
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Iron Metabolism and Disorders
- Prostate Cancer Treatment and Research
- Medical Imaging Techniques and Applications
- Complement system in diseases
- Traditional Chinese Medicine Studies
- Healthcare and Venom Research
- Glutathione Transferases and Polymorphisms
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Genetic and phenotypic traits in livestock
- Traditional Chinese Medicine Analysis
- Oral Health Pathology and Treatment
- Machine Learning in Bioinformatics
- Hemoglobinopathies and Related Disorders
- Epigenetics and DNA Methylation
- Invertebrate Immune Response Mechanisms
- Chromatography in Natural Products
- Genomics, phytochemicals, and oxidative stress
- Renal Diseases and Glomerulopathies
- Radioactive Decay and Measurement Techniques
- Pharmacological Effects of Natural Compounds
- Ovarian function and disorders
Shanghai Pulmonary Hospital
2025
Tongji University
2025
Shanghai Institute of Pharmaceutical Industry
2013-2024
Hubei University of Chinese Medicine
2024
Nanjing University of Chinese Medicine
2024
Soochow University
2021-2023
First Affiliated Hospital of Soochow University
2021-2023
Alexion Pharmaceuticals (United States)
2020-2023
AstraZeneca (United States)
2022-2023
Wuhu Fourth People Hospital
2022
Circulating microRNA (miRNA) expression profiles have been reported to be promising biomarkers for drug-induced liver injury in preclinical and clinical practice. Proper normalization is critical accurate miRNAs analysis. Herein, using SYBR green quantitative real-time PCR (RT-qPCR), we evaluated the stability of six candidate reference genes including two commonly used small RNAs (U6, 5S) four (let-7a, miR-92a, miR-103 miR-16) plasma rats with acetaminophen-induced hepatotoxicity. Data were...
Real-time RT-PCR has become a common and robust technique to detect quantify low-abundance mRNA expression is prefered tool when examining fungal gene in infected host tissues. However, correct evaluation of data requires accurate reliable normalization against reference transcript. Thus, the identification genes with stable during different conditions paramount importance. Here, we present study where vitro planta experiments were used validate stability candidates Puccinia helianthi Schw.,...
We developed a novel PCR-based pre-amplification (PreAmp) technology that can increase the abundance of over 350 target genes one million-fold. To assess potential bias introduced by PreAmp we utilized ERCC RNA reference standards, model system quantifies measurement error in analysis. assessed three types bias: amplification bias, dynamic range and fold-change bias. show our workflow introduces only minimal under stringent conditions. do detect if gene is highly abundant occurred for 16 or...
Abstract Carcinoembryonic antigen-related cell adhesion molecule1 (CEACAM1) is a tumor-associated factor that known to be involved in apoptosis, but the role of CEACAM1 cardiovascular disease unclear. We aims investigate whether influences cardiac remodeling mice with myocardial infarction (MI) and hypoxia-induced cardiomyocyte injury. Both serum patients levels were significantly increased response MI, while elevated neonatal rat cardiomyocytes (NRCs) exposed hypoxia. Eight weeks after...
Radionuclide drug conjugates (RDCs) with antibodies serve as a novel approach for the treatment of malignant tumors including glioblastoma.However, RDCs require optimal to work efficiently.Hu4G4, B7-H3-targeting humanized monoclonal IgG1 antibody, is highly specific human B7-H3 protein (a marker tumor cells, glioblastoma cells).Herein, we established 131 I-labeled hu4G4 ( I-hu4G4) and showed that it specifically bound high affinity (Kd = 0.99 ± 0.07 nM) inhibited growth U87 cells in vitro....
Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) is a sensitive and widely used technique for gene expression analysis that depends on stability of the reference genes data normalization. Tree peony ( Paeonia suffruticosa ), known as one most famous traditional ornamental plants in China, very popular both domestic international markets its showy colorful flowers. To date, no systematic studies have been performed tree with different flower colors. In this...
Legubicin is a novel conjugate of doxorubicin and legumain-cleavable peptide linker. It has been developed to ameliorate the side effects doxorubicin. Biodistribution in tumor-bearing mice, acute tolerance, potential systemic toxic Sprague-Dawley rats beagle dogs legubicin were assessed. exists mainly as protein complex plasma after entering circulation. Compared with conventional at an equal molar dose we found higher exposure tumor (approximately 1.7-fold increase) while lower normal...
Catechol-O-methyltransferase (COMT) plays a central role in DNA repair and estrogen-induced carcinogenesis. Many recent epidemiologic studies have investigated the association between COMT Val158Met polymorphism cancer risk, but results are inconclusive. In this study, we performed meta-analysis to investigate susceptibility different genetic models. Overall, no significant associations were found risk (homozygote model: odds ratio [OR] =1.05, 95% confidence interval [CI] = [0.98, 1.13];...
Immunotherapy is a valuable option for cancer treatment, and the curative effect of anti-PD-1/PD-L1 therapy correlates closely with PD-L1 expression levels. Positron emission tomography (PET) imaging feasible using 68Ga-NOTA-Nb109 nanobody. was generated by radionuclide (68Ga) labeling Nb109 NOTA chelator. To facilitate clinical trials, we explored optimal dose range in BALB/c A375-hPD-L1 tumor-burdened nude mice C57-hPD-L1 transgenic MC38-hPD-L1 administration single intravenous confirmed...
To develop a method for labeling human bone marrow mesenchymal stem cells (hMSCs) with 89Zr-oxine to characterize the biodistribution characteristics of hMSCs in normal Sprague-Dawley (SD) rats real-time by micro-PET-computed tomography (micro-PET/CT) imaging.89Zr-oxine complex was synthesized from 89Zr-oxalate and 8-hydroxyquinoline (oxine). After were labeled complex, radioactivity retention, viability, proliferation, apoptosis, differentiation, morphology, phenotype assessed. The...
Abstract ATP-citrate lyase (ACL), one of the lipogenic enzymes, catalyses formation acetyl-coenzyme A (CoA) involved in synthesis fatty acid and cholesterol. In pig, very little is known about ACL gene. this work, mRNA differential display technique was used to analyse differences gene expression between Meishan Large White pigs F1 hybrids both direct reciprocal crosses. Our results show that among differentially expressed genes up-regulated backfat hybrids. After cloning analysing...
Objective. Prostate cancer is one of the most common types found to occur in males and ranked as second-highest cause cancer-associated deaths among male patients. In this study, we have shown influence a new palladium-based anticancer agent contrast six distinct prostate lines primary cultures. Methods. used cell lines, that is, PNT2-C2, LNCaP, BPH-1, PC-3, PNT1A, P4E6. The MTP ATP assay were performed evaluate growth flow cytometry investigate status cycle. antigrowth effect palladium...
Abstract Chushi Weiling Decoction (CWD) is a classic prescription in traditional Chinese medicine used to treat dampness-heat skin diseases. However, the material composition of CWD and its therapeutic mechanism remained largely unknown. This study aimed investigate pharmacological basis their potential effects using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), gas chromatography-mass (GC-MS), network pharmacology. In this work,...
Abstract Background The non-invasive PET imaging using zrconium-89 labelled antibodys (Abs) can play important roles for personalized immunotherapeutic strategies. Hu4G4 was a humanized Abs of the transmembrance glycoprotein B7-H3, which has been approved as an attractive new target cancer immunotherapy. This study aimed to investigate potential 89 Zr hu4G4 with dose escalation design. Methods radiolabelled after modification p -SCN-deferoxamine. Zr-Immuno-PET scans U87 xenograft mice were...
Topic: 32. Platelet disorders Background: Atypical hemolytic uremic syndrome (aHUS) is a rare disease characterized by anemia, thrombocytopenia and organ damage. Ravulizumab (RAV) eculizumab (ECU) are complement C5 inhibitors (C5i) approved for the treatment of aHUS. There limited consensus on duration C5i appropriate monitoring after discontinuation patients with Aims: To understand real-world clinical practice healthcare professionals (HCPs) regarding re-initiation Methods: A double-blind...
为了解齐口裂腹鱼高迁移率蛋白B1 ( Schizothorax prenanti high mobility group box 1，SpHMGB1)的序列特征及其与嗜水气单胞菌胁迫的相关性，实验根据齐口裂腹鱼脾脏TSA库中获得的序列设计引物，采用克隆技术克隆 SpHMGB 1的核心序列并进行生物信息学分析，采用实时荧光定量PCR (qPCR)技术检测嗜水气单胞菌在体和离体胁迫后组织和巨噬细胞中 1的响应情况。序列分析结果显示， 1开放阅读框长为615 bp，编码204个氨基酸，理论分子量为23.5 ku，等电点为6.79，包含2个基本的HMG盒：A盒和B盒，以及一个酸性尾巴。SpHMGB1二级结构主要由47.06%的α-螺旋和50%的无规则卷曲构成。系统进化树分析显示，SpHMGB1与鱼类HMGB1聚为一支，与斑马鱼和鲫的亲缘关系最近，氨基酸一致性分别为90.7%和90.4%。qPCR检测结果显示，嗜水气单胞菌感染后 1具有不同的时空表达趋势，其中血液在72 h表达量最高，肾脏在6 h表达量最高，脾脏在120 h表达量最高；热灭活嗜水气单胞菌刺激巨噬细胞后，...