P2X7 is important in inflammation and tissue injury. The aim of the present study was to investigate effect inhibition, using a specific inhibitor (A438079) prevent development liver injury fibrosis mouse model fibrosis. induced by carbon tetrachloride (CCl4). Mice received subcutaneous administration vehicle (saline/olive oil), CCl4 or A438079. pro‑inflammatory pro‑fibrotic factors were determined western blot analysis. biochemistry, histopathology, collagen deposition nuclear factor‑κB...

Invasion and metastasis is the main causes leading to death of hepatocellular carcinoma (HCC) patients. However, underlying mechanism still be explored. Transforming growth factor β1 (TGF-β1) a stronger inducer HCC cell invasion. downstream effector TGF-β1 that promotes invasion unknown. In this study, we found PI3K/Akt activation takes place following stimulation TGF-β1. The inhibition abolished epithelial-mesenchymal transition (EMT) cells induced by Myocyte enhancer factors 2 (MEF2)...

Circular RNA (circRNA) is a key regulator for the malignant progression of cancer. However, role circRNA anthrax toxin receptor 1 (circANTXR1) in hepatocellular carcinoma (HCC) still unclear.Quantitative real-time PCR was performed to detect expression. Cell proliferation, migration and invasion were determined using MTT assay, EdU staining, colony formation wound healing assay transwell assay. The protein levels metastasis markers, x-ray repair cross complementing 5 (XRCC5) exosome markers...

Abstract This study aims to explore novel and reliable biomarkers for predicting hepatocellular carcinoma (HCC) prognosis. Circular RNAs (circRNAs) were determined by analysis of human circRNA arrays quantitative reverse transcription polymerase reactions. To test an interaction between circDLG1, we used luciferase reporter assays, RNA immunoprecipitation, fluorescence in situ hybridization assays that employed the miR-141-3p, WTAP. q-RT-PCR western blot evaluate target regulation miR-141-3p...

Objective: To investigate the predictive effect of major adverse cardiac events (MACE) in malignant obstructive jaundice (OJ) patients using plasma brain natriuretic peptide (BNP) level and surgical Apgar scoring (SAS) system.Methods: Forty one OJ undergoing treatments were studied at a single center. Pre-and postoperative BNP level, total bilirubin (TBil) data function (HR, CVP, CI, LVEF%) detected, SAS was calculated during surgery, relationship both with MACE after surgery...

One novel non-phosphorus multi-component composite water treatment agent was developed from polyepoxysuccinic acid, polyaspartic polyacrylic acid and benzotriazole.The performances of scale corrosion inhibition were evaluated by bubble experiments, rotary hanging-piece tests electrochemical.The testing piece characterized SEM.The results showed that the new had good performance inhibition.Further potentiodynamic polarization curves also indicated acted as a cathodic polarization-based...

Objective To explore the expression of microRNA (miRNA, miR)-449a in liver cancer, effect on migration and invasion, possible regulatory mechanism. Methods Eighteen cases cancer tissues adjacent were collected. The miR-449a tissues, 4 kinds human cell lines, normal hepatocytes was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). lines transfected with mimic negative control (NC) plasmid. after transfection RT-PCR. number invasion two groups measured Transwell...

Objective To explore the effect of microRNA(miRNA, miR)- 200c in reversing chemo- resistance human pancreatic cancer stem cells. Methods CD24+ CD44+ ESA+ cells were sorted from PANC- 1 cell line by FACS.miR-200c precursor fragment and negative control transfected into cells. The real- time fluorescent quantitative polymerase chain reaction(FQ- PCR)was used to detect miR-200c expression.MTT assay was measure drug sensitivity gemcitabine. Results CD24+ cells(0.8%) isolated...

Objective To investigate the sensitivity of human pancreatic cancer stem cells to chemotherapy and expression microRNA(miRNA, miR)- 200c in cells. Methods CD24+ CD44+ ESA+ were sorted from PANC- 1 cell line by fluorescence- activated sorter(FACS).The like properties this subpopulation assessed non- obese diabetic (NOD)/severe combined immunodeficiency(SCID) xenograft transplantation experiment.Sensitivity gemcitabine apoptosis ratio detected methyl thiazol tetrazolium(MTT)assay FACS...

Objective To explore the therapeutic effect of wortmannin on acute pancreatitis(AP) with renal dysfunction and phosphatidylinositol 3 kinase(PI3K)/protein kinase B(PKB)signaling pathway. Methods Eighty healthy male SD rats were randomly divided into normal control group, sham operation group(SO group), AP group group.SO further 3, 6 12 h sub-groups(n= 8 each).The model was established by bile duct retrograde injection.Wortmannin intraperitoneally injected 4 before operation.Serum urea...

Objective To investigate AXIN2 mRNA expression level in hepatocellular carcinoma (HCC) , and to analyze the effect of gene methylation status on its HCC genesis development. Methods Fifty-three surgical excised specimens paired adjacent non-cancerous specimens, seven normal liver five cell lines were collected. The at promoter determined by quantitative PCR. Results was lower tissues (0.1629 + 0.0679) than that (0. 4155 0. 2330), there significant difference (Z= -2. 567, P = 010). (39. 77%...

Objective To investigate effects of electricity perforation parameters on electrotransfection efficiency and survival rate B cells activated by CD40 ligand transfected with human alpha fetal protein (AFP) mRNA.Methods lymphocyte were fractionated,purified,and then recombinant soluble sCD40L.PGEM4Z/AFP/A64-EGFP plasmid was established in vitro,mixed polymerase T7RNA,and transcribed into AFP mRNA PolyA end sequence; electrotransfected sCD40L under different electric voltage ( 150-350 V),pulse...

Objective To investigate the anti-tumor efficiency of cytotoxic T-lymphocyte (CTL) induced by activated B lymphocyte after hepatocellular carcinoma (HCC) alpha fetoprotein (AFP) mRNA transfection.Methods lymphocytes were fractionated,purified and recombinant human soluble sCD40L.PGEM4Z/AFP/A64-EGFP plasmid was established in vitro,mixed with polymerase T7RNA,and then transcribed into AFP Poly (A) sequence.B electrotransfected experimental group,B GAPDH negative control group,and untreated...

Objective To study the effects of deleted in malignant brain tumours 1 (DMBT1)over-expression on sugar chain structures established gallbladder carcinoma cell line GBC-SD with stable expression DMBT1.Methods Both lectin-labeled probes,namely concanavalin (ConA) and Datura stramonium agglutinin ( DSA),were applied to detect DMBT1.Flow cytometry (FCM) was further employed types both bioti-labeled agglutinins converged surface GBC-SD/DMBT1.Reverse transcription-polymerase reaction (RT-PCR)...

Objective To observe the effect of immunotherapy on hepatocellular carcinoma (HCC) by melanoma-associated antigen-A3 (MAGE-A3) activated dendritic cells (DC)-induced antigen-specific CD8+ cytotoxic T lymphocytes (CTL) and offer help to HCC treatment. Methods After MAGE-A3 DC biomarkers, interleukin (IL)-12, IL-10 in were tested. MAGE-A3-DC was used induce CTL. The apoptosis L02 HepG2 detected after treatment with Interferon (IFN)-γ CTL enzyme-linked immunospot assay (ELISPOT)....

Objective To improve the anti-tumor effect in vitro and vivo , a bispecific antibody (CT-BiAb)was constructed. Methods CT-BiAb was obtained by overlapping polymerase chain reaction (PCR) between variable region of Trastuzumab, G4S linker Cetuximab. Pichia pastoris used to express . The binding activity CT-BiAb PANC-1 detected flow cytometry. Methyl thiazol tetrazolium (MTT) assay detect inhibition cell growth. And cytometry apoptosis after treatment with CT-BiAb. Finally, mouse tumor...

Objective To investigate the effect of miR-29c on radiosensitivity hepatoma HepG2 cells by targeting AKT2 gene. Methods The expression in human normal hepatocytes THLE-3 and cell was detected RT-PCR. The relationship between were predicted informative analysis verified dual luciferase reporter gene test Western blot. mimic/AKT2 recombinant plasmid inhibitor/ lentivirus vector shRNA transfected into Liposome 2000. irradiated with different doses (0, 2, 4, 6 8 Gy) X-rays, effects...