Chrysin is a major active ingredient of flavonoids, known to exhibit protective effects against various types cancer. However, the anticancer role chrysin hepatocellular carcinoma (HCC) and underlying molecular mechanisms remain unclear. In order evaluate on cell viability apoptosis in human HCC, HepG2 QGY7701 cells were used present study. Cell was monitored using an MTT assay. addition, Annexin V-fluorescein isothiocyanate/propidium iodide kit for labeling apoptotic cells, which then...

Dihydromyricetin (DHM) is a major active ingredient of flavonoids compounds. It exhibited anticancer activity and induced apoptosis in human hepatocellular carcinoma HepG2 cells according to our previous data. In this study, we investigated whether p53 involved DHM-triggered viability inhibition induction cancer cells. MTT [3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay was employed evaluate the after DHM treatment. Meanwhile, small interfering RNA (siRNA) adopted...

Chronic kidney disease (CKD) is acknowledged as one of the largest public health problems in world, characterized by a complex and diverse pathogenesis. Adenine-induced CKD, classical model with multiple injury mechanisms, has been extensively employed CKD research. However, complete elucidation mechanisms underlying adenine-induced remains elusive. In this study, impacts adenine (200 mg/kg/day) intake on urine metabolome rats were initially investigated using non-targeted metabolomics, then...

Helicobacter pylori (HP) eradication rates are higher by treated with the potassium-competitive acid blocker vonorasan than proton pump inhibitors (PPIs). Herein, this study analyzed clinical efficacy of vonoprazan combined high-dose amoxicillin for dual therapy in personalized HP. This retrospective analysis included 452 patients type I HP who were assigned into observation and control groups. Cytotoxin-associated gene A (CagA) vacuolating cytotoxin (VacA) antibodies detected using H....

The aim of this study was to determine the role small intestinal (SI) cytochrome P450 (P450) enzymes in metabolic activation diclofenac (DCF), a widely used nonsteroidal anti-inflammatory drug, and DCF-induced toxicity. DCF induces ulcers humans mice, but underlying mechanisms, including necessity for drug bioactivation target tissues sources identities reactive intermediates, are not fully understood. We found that number (at 50 mg/kg p.o.) significantly smaller an epithelium (IE)-specific...

The process of necroptosis mediated by tumor necrosis factor alpha (TNF-α) might play an important role in the onset and development osteonecrosis femoral head (ONFH). dysfunctions bone microvascular endothelial cells (BMECs) have been identified as part pathological processes steroid-induced ONFH. An aptamer is a single-stranded DNA or RNA oligonucleotide sequence. Previous studies designed screened various aptamers that could bind to specific targets receptors order block their...

Osteoarthritis is a chronic degenerative joint disease, and increasing evidences suggest that the pathogenic mechanism involves immune system inflammation.The aim of current study was to uncover hub genes linked infiltration in osteoarthritis synovial tissue using comprehensive bioinformatics analysis experimental confirmation.Multiple microarray datasets (GSE55457, GSE55235, GSE12021 GSE1919) for Gene Expression Omnibus database were downloaded analysis. Differentially expressed (DEGs)...

Introduction Based on the previous research that oroxylin A can suppress inflammation, we investigated hepatoprotective role of against CCl4-induced liver damage in mice and then studied possible alteration activities cytokine signaling participating regeneration. Wild type (WT) were orally administrated with (60 mg/kg) for 4 days after CCl4 injection, anti-inflammatory effects assessed directly by hepatic histology indirectly measuring serum levels aspartate aminotransferase (AST), alanine...

Hyperuricemia and dyslipidemia are involved in Cd nephrotoxicity. The aim of this study was to determine the effect quercetin, a dietary flavonoid with anti-hyperuricemic anti-dyslipidemic properties, on alteration renal UA transport system disorder lipid accumulation 3 6 mg/kg Cd-exposed rats for 4 weeks. exposure induced hyperuricemia XOR hyperactivity excretion dysfunction rats. Simultaneously, abnormal expression levels transport-related proteins including RST, OAT1, MRP4 ABCG2 were...

Hydrostatin-SN1 (peptide sequence, DEQHLETELHTLTSVLTANGFQ), a kind of peptides extracted from snake venom, has been reported to have anti-inflammatory effect, but its truncated mutant hydrostatin-SN10 DEQHLETELH) on pancreatitis-induced acute lung injury not well documented. Interleukin- (IL-) 6-induced Janus Kinase 2/Signal Transducer and Activator Transcription 3 (JAK2/STAT3) pathway is involved with inflammatory oxidative stress activities may be associated the pathogenesis injury,...

Heterotopic ossification (HO) is characterized by extraskeletal in soft tissue. Thus far, there a lack of effective drug therapy against HO. Loss PTEN osteoblasts has been reported to accumulate bone mass skeletal development and promote fracture healing association with the activation PI3K/AKT pathway. However, role PTEN/PI3K/AKT signaling HO pathogenesis remains unknown. The present study investigated implication this pathway during BMP2‑induced osteogenic differentiation ectopic...

Drug resistance is a significant concern in the treatment of diseases, including cryptococcosis caused by Cryptococcus neoformans (Cne) and gattii (Cga). Alternative drug targets are necessary to overcome before it attains critical stage. Splicing inteins from pro-protein precursors crucial for activities essential proteins hosting intein elements many organisms, human pathogens such as Cne Cga. Through high-throughput screening, we identified calcimycin (CMN) potent Prp8 splicing inhibitor...

Background: Osteonecrosis of the femoral head (ONFH) is difficult to treat, and leads an impaired microcirculation activation a repair response. The type H vessels have been proved mediate growth bone vasculature couple angiogenesis osteogenesis. aim study investigate experimental verification subtype vascular endothelial cells (HSVECs) in human head. Methods: Vascular were isolated from heads patients who underwent hip replacement. Cells by methods enzymic digestion density gradient...

Aim: To identify novel inhibitors of SIRT1 and to understand their mechanism action in hepatocellular carcinoma. Materials & methods: Molecular docking dynamic simulations were conducted potential inhibitors. The vitro efficacy the was evaluated by methyl thiazolyl tetrazolium assays, flow cytometry western blot analysis. Additionally, vivo antitumor activity inhibitor evaluated. Results: Tipranavir, a US FDA-approved anti-HIV-1 medication, found possess as inhibitor. Tipranavir selectively...

Bone microvascular endothelial cells (BMECs) constitute the central part of femoral head's intramural microenvironment network and have an essential role in development steroid‑induced osteonecrosis head. Recently, rapid microfluidic technology has led to innovations fields chemistry, medicine life sciences. It is now possible use microfluidics organ‑on‑a‑chip techniques assess osteonecrosis. In present study, BMECs were cultured on a platform explore pathogenesis femoral‑head necrosis. The...

Ovarian cancer (OC) is a highly lethal gynecological malignancy, often diagnosed at advanced stages with limited treatment options. Here, we demonstrate that the antimicrobial peptide CS-piscidin significantly inhibits OC cell proliferation, colony formation, and induces death. Mechanistically, causes necrosis by compromising membrane. Furthermore, can activate Receptor-interacting protein kinase 1 (RIPK1) induce apoptosis cleavage of PARP. To improve tumor targeting ability, modified adding...