A5100423204- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Proteins in Food Systems
- Metabolomics and Mass Spectrometry Studies
- Biochemical Analysis and Sensing Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Hydrolysis and Bioactive Peptides
- 3D Printing in Biomedical Research
- Extracellular vesicles in disease
- Virus-based gene therapy research
- Biofuel production and bioconversion
- Inflammasome and immune disorders
- Biopolymer Synthesis and Applications
- Microbial Inactivation Methods
- Proteoglycans and glycosaminoglycans research
- Plasma Applications and Diagnostics
- Garlic and Onion Studies
- Immunotherapy and Immune Responses
- Carbohydrate Chemistry and Synthesis
- Phytase and its Applications
- Microbial metabolism and enzyme function
- Inflammatory Bowel Disease
- Transgenic Plants and Applications
- Vibrio bacteria research studies
- GABA and Rice Research
Hangzhou Medical College
2025
Zhejiang Provincial People's Hospital
2025
South China University of Technology
2022-2024
Tianjin University of Science and Technology
2024
Heze University
2024
Beijing National Laboratory for Molecular Sciences
2022
China Pharmaceutical University
2022
Chinese Academy of Sciences
2022
University of Chinese Academy of Sciences
2022
Academy of State Administration of Grain
2021
Abstract Background Komagataella phaffii , a type of methanotrophic yeast, can use methanol, favorable non-sugar substrate in eco-friendly bio-manufacturing. The dissimilation pathway K. leads to the loss carbon atoms form CO 2 . However, ΔFLD strain, engineered lack formaldehyde dehydrogenase—an essential enzyme pathway—displayed growth defects when exposed methanol-containing medium. Results Inhibiting triggers an excessive accumulation and decline intracellular NAD + /NADH ratio. Here, we...
Summary The inactivation of polyphenol oxidase (PPO) in peach juice and PPO carrot was investigated by high‐pressure carbon dioxide (HPCD), their kinetics analysed compared. temperature 35–55 °C, the pressure 5–15 MPa under HPCD condition. Results showed that enhanced effect on two PPOs. well fitted to a first‐order kinetic model, fraction‐conversion model as function temperatures or pressures. Susceptibility rate constant k not altered lessened temperature, but susceptibility changed when...
The aggregation of β-amyloid peptide (Aβ) is one potential cause for Alzheimer's disease (AD). Heparin can either promote or inhibit Aβ aggregation. sulfation pattern and chain size determine its binding affinity role. Using 2D-NMR analysis molecular modelling, the motif heparin oligoaccharides to was determined be HexA-GlcNS-IdoA2S-GlcNS6S. Iduronic acid epimerization 6-O-sulfation are key factors affinity, while 3-O-sulfation Arixtra (heparin pentasaccharide) not involved in Aβ....
High methanol-tolerant strains were obtained using iterative adaptive laboratory evolution (microbial microdroplet culture (MMC) and shake flask (SFC)).
This study optimized the process of extracting protein from black garlic using an alkaline dissolution and acid precipitation method through response surface methodology. The optimal extraction conditions were determined as a solid-to-liquid ratio 1:50, time 100 min, temperature 30 °C, pH 9.0. Under these conditions, actual (BGP) yield was 12.10% ± 0.21%, isoelectric point obtained BGP 3.1. Subsequently, this extracted under subjected it to enzymatic hydrolysis different enzymes (trypsin,...