A5011293326- Peroxisome Proliferator-Activated Receptors
- Chromatin Remodeling and Cancer
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Cancer Mechanisms and Therapy
- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- 14-3-3 protein interactions
- Helicobacter pylori-related gastroenterology studies
- Genetic factors in colorectal cancer
- Neurological Disease Mechanisms and Treatments
- Inflammatory mediators and NSAID effects
- Immune cells in cancer
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Traditional Chinese Medicine Analysis
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Spectroscopy Techniques in Biomedical and Chemical Research
- DNA and Nucleic Acid Chemistry
- Drug Transport and Resistance Mechanisms
University of Sannio
2016-2025
Ceinge Biotecnologie Avanzate (Italy)
2012
University of Naples Federico II
1999-2010
Zero to Three
2003
Federico II University Hospital
2000-2002
MicroRNA-130b (miR-130b) is involved in several biologic processes; its role colorectal tumorigenesis has not been addressed so far. Herein, we demonstrate that miR-130b up-regulation exhibits clinical relevance as it linked to advanced cancers (CRCs), poor patients' prognosis, and molecular features of enhanced epithelial-mesenchymal transition (EMT) angiogenesis. high-expressing cells develop large, dedifferentiated, vascularized tumors mouse xenografts, are reverted by intratumor...
5-Fluorouracil (5-FU) is a key component of chemotherapy for colorectal cancer (CRC). 5-FU efficacy established by intracellular levels folate cofactors and DNA damage repair strategies. However, drug resistance still represents major challenge. Here, we report that alterations in serine metabolism affect sensitivity vitro vivo CRC models. In particular, 5-FU-resistant cells display strong dependency achieved either upregulating endogenous synthesis or increasing exogenous uptake....
Peroxisome proliferator-activated receptor (PPAR)-gamma is a major regulator of adipogenesis and insulin sensitivity. The PPAR-gamma gene generates two isoforms through alternative splicing, PPAR-gamma1 -gamma2, the latter having an additional stretch 28 amino acids at its NH2-terminus in ligand-independent activation domain. This extension renders PPAR-gamma2 more sensitive to action. Since there Pro12Ala substitution this domain, we tested whether it related type 2 diabetes or resistance....
Abstract Impairment of the immune response and aberrant expression microRNAs are emerging hallmarks tumour initiation/progression, in addition to driver gene mutations epigenetic modifications. We performed a preliminary survey independent adenoma colorectal cancer (CRC) miRnoma data sets and, among most dysregulated miRNAs, we selected miR-27a disclosed that it is already upregulated further increases during evolution adenocarcinoma. To identify novel genes pathways regulated by this miRNA,...
Immunogenic cell death (ICD) evoked by chemotherapeutic agents implies emission of selected damage-associated molecular patterns (DAMP) such as surface exposure calreticulin, secretion ATP and HMGB1. We sought to verify whether miR-27a is implicated in ICD, having demonstrated that it directly targets calreticulin. To this goal, we exposed colorectal cancer lines, genetically modified express high or low levels, two bona fide ICD inducers (mitoxantrone oxaliplatin). Low miR-27a-expressing...
Abstract Background Metabolic reprogramming towards aerobic glycolysis in cancer supports unrestricted cell proliferation, survival and chemoresistance. The molecular bases of these processes are still undefined. Recent reports suggest crucial roles for microRNAs. Here, we provide new evidence the implication miR-27a modulating colorectal (CRC) metabolism Methods A survey expression profile TCGA-COAD dataset revealed that miR-27a-overexpressing CRCs enriched gene signatures mitochondrial...
Fibrates are hypolipidemic drugs that activate the peroxisome proliferator‐activated receptors. Since fibrates may also increase energy expenditure, we investigated whether fenofibrate (FF) had this effect in diet‐induced obese rats. A 2‐month administration of a high‐fat palatable diet to adult rats increased body weight by 25% and white adipose mass 163% compared with standard diet. These effects were prevented FF, both when administered for 2 months feeding given only second month....
The relationship between peroxisome proliferator-activated receptor γ (PPARG) expression and epigenetic changes occurring in colorectal-cancer pathogenesis is largely unknown. We investigated whether PPARG epigenetically regulated colorectal cancer (CRC) progression. was assessed CRC tissues paired normal mucosa by western blot immunohistochemistry related to patients' clinicopathological parameters survival. promoter methylation analyzed methylation-specific-PCR bisulphite sequencing. were...
Aberrant DNA methylation often occurs in colorectal cancer (CRC). In our study we applied a genome-wide analysis approach, MethylCap-seq, to map the differentially methylated regions (DMRs) 24 tumors and matched normal colon samples. total, 2687 frequently hypermethylated 468 hypomethylated were identified, which include potential biomarkers for CRC diagnosis. Hypermethylation tumor samples was enriched at CpG islands gene promoters, while hypomethylation distributed throughout genome. Using...
Circadian disruption and deranged molecular clockworks are involved in carcinogenesis. The cryptochrome genes (CRY1 CRY2) encode circadian proteins important for the functioning of biological oscillators. Their expression human colorectal cancer (CRC) colon cell lines has not been evaluated so far. We investigated CRY1 CRY2 fifty CRCs CaCo2, HCT116, HT29, SW480 lines. (p = 0.01) < 0.0001) was significantly changed tumour tissue, as confirmed a large independent CRC dataset. In addition,...
We examined the peroxisome proliferator-activated receptor gamma (PPARG) locus in an attempt to identify expressed sequence tags and/or conserved non-coding sequences intron containing open reading frames and potentially able encode new proteins. identified a PPARG transcript, defined gammaORF4, which harbors readthrough 4. The expected translated protein lacks ligand-binding domain encoded by exons 5 6. transcript human tumor cell lines tissues, synthesized cDNA, cloned it expression...
We describe a novel bioinformatic and translational pathology approach, gene Signature Finder Algorithm (gSFA) to identify biomarkers associated with Colorectal Cancer (CRC) survival. Here robust set of CRC markers is selected by an ensemble method. By using dataset 232 expression profiles, gSFA discovers 16 highly significant small signatures. Analysis dichotomies generated the signatures results in 133 samples stably classified good prognosis group 56 poor group, whereas 43 remain...
Taurisolo® is a pomace extract from Aglianico Grapes, wine cultivar native to Campania (Southern Italy). It exhibits very high polyphenolic content and, consumed as nutraceutical, effective in reducing the level of Trimethylamine N-oxide (TMAO), cardiovascular diseases risk factor marker. We here show effects on rat brain microvascular alterations induced by diminution cerebral blood flow (CBFD) for 30 min, due bilateral common carotid artery occlusion, and subsequent restoration (CBFR) 60...
Tumors of the gastrointestinal tract are among most frequent human malignancies and account for approximately 30% cancer-related deaths worldwide. Peroxisome proliferator-activated receptors (PPARs) ligand-activated transcription factors that control diverse cellular functions such as proliferation, differentiation, cell death. Owing to their involvement in so many processes, they play crucial roles also development physiology tract. Consistently, PPARs deregulation has been implicated...
Peroxisome proliferator-activated receptor gamma (PPAR γ ) plays critical roles in lipid storage, glucose metabolism, energy homeostasis, adipocyte differentiation, inflammation, and cancer. Its function colon carcinogenesis has largely been debated; accumulating evidence, however, supports a role as tumor suppressor through modulation of crucial pathways cell apoptosis, metastatic dissemination. Epigenetics adds further layer complexity to gene regulation several biological processes. In...