A5083957043- Vector-borne infectious diseases
- Viral Infections and Vectors
- Planarian Biology and Electrostimulation
- Mosquito-borne diseases and control
- Toxoplasma gondii Research Studies
- Marine Ecology and Invasive Species
- Mycobacterium research and diagnosis
- Bacterial biofilms and quorum sensing
- Plant and Biological Electrophysiology Studies
- Legionella and Acanthamoeba research
- Tuberculosis Research and Epidemiology
- Whipple's Disease and Interleukins
- Genomics and Phylogenetic Studies
- Vector-Borne Animal Diseases
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Bartonella species infections research
- Rabies epidemiology and control
- Invertebrate Immune Response Mechanisms
- Cellular transport and secretion
- Aquaculture disease management and microbiota
- Streptococcal Infections and Treatments
- Autophagy in Disease and Therapy
- Venomous Animal Envenomation and Studies
- bioluminescence and chemiluminescence research
Méditerranée Infection Foundation
2016-2022
Aix-Marseille Université
2011-2021
Institut de Recherche pour le Développement
2010-2021
Centre National de la Recherche Scientifique
2009-2018
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2009-2018
Inserm
2012-2018
Délégation Provence et Corse
2018
Walter and Eliza Hall Institute of Medical Research
2016
University of Turin
2012
Institut de Neurobiologie de la Méditerranée
1999-2011
Here we report the discovery of two Tupanvirus strains, longest tailed Mimiviridae members isolated in amoebae. Their genomes are 1.44-1.51 Mb linear double-strand DNA coding for 1276-1425 predicted proteins. Tupanviruses share same ancestors with mimivirus lineages and these giant viruses present largest translational apparatus within known virosphere, up to 70 tRNA, 20 aaRS, 11 factors all translation steps, related tRNA/mRNA maturation ribosome protein modification. Moreover, sequences...
Macrophages are critical components of the innate and adaptive immune responses, they first line defense against foreign invaders because their powerful microbicidal activities. widely distributed throughout body present in lymphoid organs, liver, lungs, gastrointestinal tract, central nervous system, bone, skin. Because repartition, participate a wide range physiological pathological processes. highly versatile cells that able to recognize microenvironmental alterations maintain tissue...
Mimivirus, or Acanthamoeba polyphaga mimivirus (APMV), a giant double-stranded DNA virus that grows in amoeba, was identified for the first time 2003. Entry by phagocytosis within amoeba has been suggested but not demonstrated. We demonstrate here APMV internalized macrophages non-phagocytic cells, leading to productive replication. Clathrin- and caveolin-mediated endocytosis pathways, as well degradative endosome-mediated endocytosis, were used invade macrophages. Ultrastructural analysis...
Background. Necrotizing enterocolitis (NEC) is the most common and serious gastrointestinal disorder among preterm neonates. We aimed to assess a specific gut microbiota profile associated with NEC.
Abstract The subversion of microbicidal functions macrophages by intracellular pathogens is critical for their survival and pathogenicity. replication Coxiella burnetii, the agent Q fever, in acidic phagolysosomes nonphagocytic cells has been considered as a paradigm life bacteria. We show this study that C. burnetii THP-1 monocytes was not related to phagosomal pH because bacterial vacuoles were independently virulence. In contrast, virulent escapes killing resting preventing phagosome...
Macrophages are critical components of the innate and adaptive immune responses, they first line defense against foreign invaders because their powerful microbicidal activities. widely distributed throughout body present in lymphoid organs, liver, lungs, gastrointestinal tract, central nervous system, bone, skin. Because repartition, participate a wide range physiological pathological processes. highly versatile cells that able to recognize microenvironmental alterations maintain tissue...
Abstract The role of Toll-like receptors (TLRs) in the recognition extracellular and facultative intracellular bacteria by innate immune system has been extensively studied, but their obligate organisms remains unknown. Coxiella burnetii, agent Q fever, is an bacterium that specifically inhabits monocytes/macrophages. We showed this study C. burnetii LPS involved uptake virulent macrophages not avirulent variants. was dependent on TLR4 because it reduced from TLR4−/− mice. In addition,...
Orientia tsutsugamushi is the causal agent of scrub typhus, a public health problem in Asia-Pacific region and life-threatening disease. O. an obligate intracellular bacterium that mainly infects endothelial cells. We demonstrated here also replicated monocytes isolated from healthy donors. In addition, altered expression more than 4,500 genes, as by microarray analysis. The type I interferon, interferon-stimulated genes associated with M1 polarization macrophages was significantly...
Q fever is a zoonosis caused by Coxiella burnetii, an obligate intracellular bacterium typically found in myeloid cells. The infection source of severe obstetrical complications humans and cattle can undergo chronic evolution minority pregnant women. Because C. burnetii the placentas aborted fetuses, we investigated possibility that it could infect trophoblasts. Here, show infected replicated BeWo trophoblasts within phagolysosomes. Using pangenomic microarrays, induced specific...
Abstract Pregnancy is dependent on maternal–fetal tolerance that may be compromised because of infections or inflammation the placenta. In this study, we examined whether context placental immune affected functions resident macrophages and if their were altered during chorioamnionitis, an infectious pathology Macrophages from at-term placentas expressed CD14, exhibited macrophage microbicidal functions, but less inflammatory than monocyte-derived macrophages. Moreover, spontaneously matured...
Q fever manifests as primary infection or acute and may become chronic in patients with underlying valvulopathy. Because Coxiella burnetii depends on host response, we measured tumor necrosis factor (TNF), interleukin (IL)–6 , IL-12, IL-10 different clinical presentations of fever. Compared control subjects, uncomplicated exhibited increased release the 4 cytokines. Their amounts were higher hepatitis than pneumonia. In valvulopathy, who highest risk evolution, TNF without production was...
Tropheryma whipplei was established as the agent of Whipple's disease in 2000, but mechanisms by which it survives within host cells are still unknown. We show here that T. HeLa controlling biogenesis its phagosome. Indeed, colocalized with lysosome-associated membrane protein 1, a marker late endosomal and lysosomal compartments, not cathepsin D, hydrolase. This defect phagosome maturation is specific to live organisms, since heat-killed bacilli D. In addition, survived adapting acidic pH....
Coxiella burnetii, an obligate intracellular bacterium, is the agent of Q fever. The chronic form disease associated with overproduction interleukin-10 and deficient C. burnetii killing by monocytes. We hypothesized that replication inside monocytes requires a macrophage-deactivating cytokine such as interleukin-10. In absence interleukin-10, survived but did not replicate in (measured 15 days) was induced interleukin-10-treated This effect specific since transforming growth factor beta1 had...
Organophosphorus insecticides (OPs) are toxic compounds used for agricultural purposes and responsible severe types of contamination worldwide. OPs may also induce chronic deleterious effects developmental disruption. Finding remediation strategies is a major concern to diminish their impact on environment human health. Enzymes have emerged as promising eco-friendly route decontaminating OPs. The enzyme SsoPox from the archaea Sulfolobus solfataricus has been particularly studied,...
Dendritic cells (DCs) orchestrate host defenses against microorganisms. In infectious diseases due to intracellular bacteria, the inefficiency of immune system eradicate microorganisms has been attributed hijacking DC functions. this study, we selected bacterial pathogens with distinct lifestyles and explored responses monocyte-derived DCs (moDCs). Using lipopolysaccharide as a control, found that Orientia tsutsugamushi, causative agent scrub typhus survives in cytosol target cells, induced...
Mycobacterium abscessus is an emerging human pathogen causing severe pulmonary infections and refractory to standard antibiotherapy, yet few drug resistance mechanisms have been reported in this organism. Recently, mutations MAB_4384 leading up-regulation of the MmpS5/MmpL5 efflux pump were linked increased thiacetazone derivatives. Herein, DNA-binding activity was investigated by electrophoretic mobility shift assays using palindromic sequence IRS5/L5 located upstream mmpS5/mmpL5....