A5019760324- Biosensors and Analytical Detection
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Biochemical and Structural Characterization
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Bacterial biofilms and quorum sensing
- Viral Infections and Vectors
- Salmonella and Campylobacter epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- Bacterial Identification and Susceptibility Testing
- Advanced Nanomaterials in Catalysis
- Poxvirus research and outbreaks
- Circular RNAs in diseases
- Animal health and immunology
- Hepatitis B Virus Studies
- Rabies epidemiology and control
- Aquaculture disease management and microbiota
- Wound Healing and Treatments
- Vibrio bacteria research studies
- Advanced Biosensing Techniques and Applications
Chinese Academy of Medical Sciences & Peking Union Medical College
2021-2024
Kunming University of Science and Technology
2018-2024
First People's Hospital of Yunnan Province
2024
Second People's Hospital of Yunnan Province
2021
Jilin University
2020
First Hospital of Jilin University
2020
Shigella flexneri is a serious threat to global public health, and rapid detection method urgently needed. The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system widely used in gene editing, therapy, vitro diagnosis. Here, we combined loop-mediated isothermal amplification (LAMP) CRISPR/Cas12a develop novel diagnostic test (CRISPR/Cas12a-E-LAMP) for the diagnosis of S. . CRISPR/Cas12a-E-LAMP protocol conducts LAMP reaction templates followed by...
Respiratory infections pose a serious threat to global public health, underscoring the urgent need for rapid, accurate, and large-scale diagnostic tools. In recent years, CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system, combined with isothermal amplification methods, has seen widespread application in nucleic acid testing (NAT). However, achieving single-tube reaction system containing all necessary components is challenging due competitive...
Antimicrobial peptides (AMPs) are a class of templates with application potential for drug development. Amphibians important sources AMPs. Duttaphrynus melanostictus is the main source traditional Chinese medicine "Chansu", which has anti-infection effect while without clear mechanism. This study aimed to find cathelicidin peptide in D. and then investigate activity vivo vitro, an AMP-encoding gene (cathelicidin-DM, GenBank: KJ820824.1) was obtained from constructed cDNA library...
Over the past decade, increasing evidence has shown that circular RNAs (circRNAs) play important regulatory roles in viral infection and host antiviral responses. However, reports on role of circRNAs Zika virus (ZIKV) are limited. In this study, we identified 45 differentially expressed ZIKV-infected A549 cells by RNA sequencing. We clarified a downregulated circRNA, hsa_circ_0007321, regulates ZIKV replication through targeting miR-492 downstream gene NFKBID. NFKBID is negative regulator...
Pseudomonas aeruginosa is one of the three most pathogenic bacteria that frequently cause life‑threatening opportunistic human infections, pneumonia, and lower respiratory tract infections in immunocompromised hosts, particularly burns ward. The present study aimed to establish a loop‑mediated isothermal amplification (LAMP) method for rapid sensitive detection P. aeruginosa‑specific gene hypothetical protein (GenBank ID: 882161). was obtained through local online BLAST, specific primers...
Escherichia coli, Staphylococcus aureus, Shigella, Pseudomonas aeruginosa, and Klebsiella pneumoniae are common foodborne pathogens. In this study, the light-induced PMAxx-coupled multiplex PCR (PMAxx-mPCR) was established to detect aforementioned five pathogens in fresh juice at same time. Moreover, PMAxx pretreatment could effectively distinguish live bacteria from dead bacteria. The optimized conditions were incubation with a final concentration of 10 μmol/L for min then photolysis 8 min....
Abstract Cytidine/uridine monophosphate kinase 2 (UMP‐CMP 2, CMPK2) has been reported as an antiviral interferon‐stimulated gene (ISG). We previously observed that the expression of CMPK2 was significantly upregulated after Zika Virus (ZIKV) infection in A549 cells. However, association and underlying mechanisms between induction ZIKV replication remain to be determined. investigated during effect on A549, U251, Vero, IFNAR‐deficient U5A its parental 2fTGH cells, Huh7 RIG‐I‐deficient...
Klebsiella pneumoniae is a common human pathogen that causes several nosocomial chronic infections. Many methods have been reported to detect pneumonia; however, simple, rapid, and visual method remains be developed. Here, we developed detection employing nanoparticle immunosensor (NPIS) with sensitivity of 8 CFU mL-1. Two monoclonal antibodies (mAbs) recognizing different antigenic determinants on the surface pneumonia were screened by an indirect enzyme-linked immunosorbent assay (ELISA),...
Shigella flexneri (S. flexneri) is prevalent worldwide and the most common in many countries, causing highly contagious diarrhea, which seriously threatens public health. This study aimed to develop a colorimetric loop-mediated isothermal amplification (LAMP) for rapid, accurate, visualization detection of S. flexneri.According screened specific genes flexneri, three groups LAMP primers were designed evaluated, reaction volume was optimized. The specificity validated by 20 96 non-S. clinical...
Abstract A small percentage of couples who regularly donated blood in China tested positive for HBsAg. Although it is well known that donors can acquire hepatitis B virus (HBV) infection from a chronically infected sexual partner, the prevalence occult infections (OBIs) among donations partners HBV‐infected spouses and risk to safety remain poorly understood. Among 212 763 donors, 54 pairs (108 donations) were enrolled because one partner Several molecular serological examinations conducted....
In recent years, food safety incidents caused by Escherichia coli have occurred and endangered human health. Due to the complex matrix of milk samples long pretreatment time, existing methods cannot quickly detect E. in samples. It is necessary enrich improve detection sensitivity. The outer membrane protein A (OmpA) widely present on cell may be used as a new target coli. this study, purified recombinant OmpA was immunize BALB/c mice produce polyclonal antibody. Immunomagnetic beads were...
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, rapid accurate detection K. important for timely treatment infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method sensitive pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The was obtained through local online BLAST, specific primers were designed its detection. Positive...
Background: Klebsiella pneumoniae is an important human pathogen that causes severe diseases including urinary tract infection, pneumonia, and bacteremia. However, a rapid sensitive detection method remains to be developed. Objectives: This study aimed develop rapid, real-time, visual for K. pneumoniae. primary screening improve the diagnosis of infection save much precious time clinical practice. Methods: was used as antigen produce monoclonal antibodies (mAb). The mAb 1E6 recognizing outer...
Escherichia coli is the most common pathogenic bacteria that frequently causes life-threatening opportunistic human infections, diarrhea, and septicemia in immunocompromised hosts.This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for rapid sensitive detection of hypothetical protein from an E. coli-specific gene (GenBank ID: 13702648). The was obtained through local online BLAST, specific primers were designed this gene. Reaction conditions optimized at...
Hepatocellular carcinoma (HCC) is a highly aggressive tumor with limited treatment options and high mortality. Senecavirus A (SVA) has shown potential in selectively targeting tumors while sparing healthy tissues. This study aimed to investigate the effects of SVA on HCC cells
CRISPR-Cas technology is a widely utilized gene-editing tool that involves gRNA-guided sequence recognition and Cas nuclease-mediated cleavage. The design evaluation of gRNA are essential for enhancing CRISPR/Cas editing efficiency. Various assays such as single-strand annealing,
Antibiotics are highly effective drugs used in the treatment of infectious diseases. Aminoglycoside antibiotics one most common bacterial infections. However, development drug resistance against those medicines is becoming a serious concern.This study aimed to develop an efficient, rapid, accurate, and sensitive detection method that applicable for routine clinical use.Escherichia coli was as model organism reliable multiplex polymerase chain reaction (M-PCR) four aminoglycoside modifying...
Background: Staphylococcus aureus and coagulase-negative staphylococci are highly antibiotic-resistant pathogens exhibit multiple virulence factors that threaten public health. However, few reports have evaluated the resistance of in community settings. Objectives: This study surveyed epidemiology, antimicrobial resistance, genes densely-populated university town Kunming Southwestern China. Methods: Various strains were isolated from hand-touching surfaces Chenggong University Town, China,...
Abstract: Background : Cytidine/uridine monophosphate kinase 2 (UMP-CMP 2, CMPK2) has been reported as an antiviral interferon-stimulated gene (ISG). We previously observed that the expression of CMPK2 was significantly upregulated after Zika Virus (ZIKV) infection in A549 cells. However, role ZIKV replication remains to be determined. Methods or retinoic acid inducible I (RIG-I) overexpressed by plasmid transfection knocked down siRNA, respectively cells before were infected with ZIKV. RNA...