A5087866685- Viral Infections and Vectors
- Mosquito-borne diseases and control
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Virus-based gene therapy research
- Long-Term Effects of COVID-19
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- HIV Research and Treatment
- Liver physiology and pathology
- Retinoids in leukemia and cellular processes
- Viral Infections and Outbreaks Research
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2024
Massachusetts General Hospital
2016-2024
Harvard University
2016-2024
Zunyi Medical University
2022
Abstract HCV replication disrupts normal endoplasmic reticulum (ER) function and activates a signaling network called the unfolded protein response (UPR). UPR is directed by three ER transmembrane proteins including ATF6, IRE1 PERK. increases TGF-β1 oxidative stress, which play important roles in liver fibrogenesis. has been shown to induce through generation of reactive oxygen species (ROS) p38 MAPK, JNK, ERK1/2 NFκB-dependent pathways. However, relationship between HCV-induced stress...
Long noncoding RNAs (lncRNAs) play a critical role in the regulation of many important cellular processes. However, mechanisms by which lncRNAs regulate viral infection and host immune responses are not well understood. We sought to explore lncRNA hepatitis C virus (HCV) interferon response. performed RNA sequencing (RNAseq) Huh7.5.1 cells with or without alpha (IFNα) treatment. Clustered regularly interspaced short palindromic repeats/Cas9 guide (gRNA) was used knock out selected genes. The...
Shigella flexneri is a serious threat to global public health, and rapid detection method urgently needed. The CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system widely used in gene editing, therapy, vitro diagnosis. Here, we combined loop-mediated isothermal amplification (LAMP) CRISPR/Cas12a develop novel diagnostic test (CRISPR/Cas12a-E-LAMP) for the diagnosis of S. . CRISPR/Cas12a-E-LAMP protocol conducts LAMP reaction templates followed by...
Respiratory infections pose a serious threat to global public health, underscoring the urgent need for rapid, accurate, and large-scale diagnostic tools. In recent years, CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system, combined with isothermal amplification methods, has seen widespread application in nucleic acid testing (NAT). However, achieving single-tube reaction system containing all necessary components is challenging due competitive...
Persons with chronic HBV infection coinfected HIV experience accelerated progression of liver fibrosis compared to those monoinfection. We aimed determine whether and its proteins promote HBV-induced in HIV/HBV-coinfected cell culture models through HIF-1α TGF-β1 signaling.
Aging | doi:10.18632/aging.202877. Shasha Li, Xiaoqiong Duan, Yujia Ming Yong Gao, Tuantuan Shilin Lin Tan, Tuo Shao, Andre J. Jeyarajan, Limin Chen, Mingfeng Han, Wenyu Lin, Xiuyong Li
Background: 2′, 5′-oligoadenylate synthetase 2 (OAS2) has been known as an antiviral interferon-stimulated gene (ISG). However, the role of OAS2 on Zika virus (ZIKV) replication is still unknown. In this study, we sought to explore effect ZIKV and its underlying mechanism. Methods: We performed RNA-Seq in A549 cells with or without infection. RIG-I was overexpressed by plasmid transfection knocked down siRNA cells. Expression levels mRNA protein selected genes were detected RT-qPCR Western...
Hepatitis B virus (HBV)/hepatitis C (HCV) coinfection accelerates liver fibrosis progression compared with HBV or HCV monoinfection. Octamer binding transcription factor 4 (OCT4) and Nanog are direct targets of the profibrogenic TGF-β1 signaling cascade. We leveraged a coculture model to monitor effects on fibrogenesis in both sodium taurocholate cotransporting polypeptide-transfected Huh7.5.1 hepatoma cells LX2 hepatic stellate (HSCs). used CRISPR-Cas9 knock out OCT4 evaluate their HBV-,...
Abstract Background: Human umbilical cord mesenchymal stem cells (hUCMSCs) are promising seed in bone tissue engineering. circRNA and N6-methyladenosine (m6A) RNA methylation play important roles osteogenic differentiation. Here, we investigated the potential relevance of a critical circRNA, hsa_circ_0003376 (circCTTN), methyltransferase-like 3 (METTL3) differentiation hUCMSCs. Methods: Expression circCTTN after hUCMSC induction was detected by qRT-PCR. Three databases (RMBase v2.0, BERMP,...
We previously identified that miR-130a downregulates HCV replication through two independent pathways: restoration of host immune responses and regulation pyruvate metabolism. In this study, we further sought to explore antiviral target genes regulated by miR-130a. performed a RT² Profiler™ PCR array identify the The putative binding sites between its downregulated were predicted miRanda. over-expressed or knocked down siRNA CRISPR/Cas9 gRNA. Selected gene mRNAs their proteins, together with...
Previous studies demonstrated that dengue virus (DENV) infection developed resistance to type-I interferons (IFNα/β). The underlying mechanism remains unclear. USP18 is a negative regulator of IFNα/β signaling, and its expression level significantly increased following DENV in cell lines patients’ blood. Our previous study revealed contributed the IFN-α Hepatitis C Virus (HCV). However, role replication elusive. In this current study, we aimed explore DENV-2 IFN-α. was up-regulated by...
Over the past decade, increasing evidence has shown that circular RNAs (circRNAs) play important regulatory roles in viral infection and host antiviral responses. However, reports on role of circRNAs Zika virus (ZIKV) are limited. In this study, we identified 45 differentially expressed ZIKV-infected A549 cells by RNA sequencing. We clarified a downregulated circRNA, hsa_circ_0007321, regulates ZIKV replication through targeting miR-492 downstream gene NFKBID. NFKBID is negative regulator...
Background MicroRNAs (miRNAs) play critical roles in regulating virus infection and replication. However, the mechanism by which miRNA regulates Zika (ZIKV) replication remains elusive. We aim to explore how differentially expressed miR-103a-3p ZIKV clarify underlying molecular mechanism. Methods Small RNA sequencing (RNA-Seq) was performed identify miRNAs A549 cells with or without some of dysregulated were validated quantitative real time PCR (qRT-PCR). The effect on examined transfecting...
Abstract Cytidine/uridine monophosphate kinase 2 (UMP‐CMP 2, CMPK2) has been reported as an antiviral interferon‐stimulated gene (ISG). We previously observed that the expression of CMPK2 was significantly upregulated after Zika Virus (ZIKV) infection in A549 cells. However, association and underlying mechanisms between induction ZIKV replication remain to be determined. investigated during effect on A549, U251, Vero, IFNAR‐deficient U5A its parental 2fTGH cells, Huh7 RIG‐I‐deficient...
Shigella flexneri (S. flexneri) is prevalent worldwide and the most common in many countries, causing highly contagious diarrhea, which seriously threatens public health. This study aimed to develop a colorimetric loop-mediated isothermal amplification (LAMP) for rapid, accurate, visualization detection of S. flexneri.According screened specific genes flexneri, three groups LAMP primers were designed evaluated, reaction volume was optimized. The specificity validated by 20 96 non-S. clinical...
Mitophagy is a selective degradation mechanism that maintains mitochondrial homeostasis by eliminating damaged mitochondria. Many viruses manipulate mitophagy to promote their infection, but its role in Zika virus (ZIKV) unclear. In this study, we investigated the effect of activation on ZIKV replication uncoupling agent niclosamide. Our results demonstrate niclosamide-induced inhibits fragmented mitochondria, both vitro and mouse model ZIKV-induced necrosis. Niclosamide induces...
Abstract Background Although interferon regulatory factor 2 (IRF2) was reported to stimulate virus replication by suppressing the type I signaling pathway, because cell cycle arrest found promote viral replication, IRF2-regulated fork (FAM111A and RFC3) might be able affect ZIKV replication. In this study, we aimed investigate function of IRF2, FAM111A RFC3 underlying mechanism. Methods siIRF2, siFAM111A, siRFC3 pIRF2 in ZIKV-infected A549, 2FTGH U5A cells were used explore mechanism IRF2...
Ran-specific binding protein 1 (RANBP1) is involved in the regulation of cell cycle, while its role hepatocellular carcinoma (HCC) unknown. Therefore, we aimed to demonstrate association RANBP1 with clinicopathologic features and potential biological functions HCC based on The Cancer Genome Atlas (TCGA) data.
Aim: TMEM2 has been demonstrated to suppress HBV infection by activating the Jak/STAT pathway. In this study, we sought explore mechanism which effects on Zika virus (ZIKV) replication. Materials & methods: was overexpressed. Selected gene mRNA, p-STAT1 levels and interferon stimulated response element activity were examined qRT-PCR, Western blot luciferase assay respectively. Results: Overexpression of significantly inhibited ZIKV replication, upregulated MDA5 RIG-I expression, increased...