A5014529493- CRISPR and Genetic Engineering
- interferon and immune responses
- Hepatitis B Virus Studies
- HIV Research and Treatment
- Advanced biosensing and bioanalysis techniques
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Blood donation and transfusion practices
- Autophagy in Disease and Therapy
- Hepatitis C virus research
- Blood groups and transfusion
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2025
China XD Group (China)
2023-2025
Guangzhou Blood Center
2023-2025
Respiratory infections pose a serious threat to global public health, underscoring the urgent need for rapid, accurate, and large-scale diagnostic tools. In recent years, CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system, combined with isothermal amplification methods, has seen widespread application in nucleic acid testing (NAT). However, achieving single-tube reaction system containing all necessary components is challenging due competitive...
ABSTRACT Utilize 16S rRNA sequencing technology to characterize bacterial species susceptible people living with HIV (PLWH) across different stages. This mapping aims establish a foundational framework for preventing secondary infections, prolonging patient survival, enhancing quality of life, and advancing the diagnosis, treatment, research co‐infections. In this study, we classified participants into three groups: The blood donors (DI group), AIDS patients who have received ART treatment...
ABSTRACT In our previous studies, 45.2% of donations with HBV HBsAg reactive (+) by ELISA but negative (−) NAT were confirmed to have infections in Shenzhen Blood Center, China. However, the serological and molecular characteristics this type infection remain unclear, several indeterminate results require further investigation. current study, blood ELISA+/DNA NAT− collected characterized various tests, quantitative PCR (qPCR) nested from January, 2019 December, 2021. Molecular...
Abstract Cytidine/uridine monophosphate kinase 2 (UMP‐CMP 2, CMPK2) has been reported as an antiviral interferon‐stimulated gene (ISG). We previously observed that the expression of CMPK2 was significantly upregulated after Zika Virus (ZIKV) infection in A549 cells. However, association and underlying mechanisms between induction ZIKV replication remain to be determined. investigated during effect on A549, U251, Vero, IFNAR‐deficient U5A its parental 2fTGH cells, Huh7 RIG‐I‐deficient...
Abstract A small percentage of couples who regularly donated blood in China tested positive for HBsAg. Although it is well known that donors can acquire hepatitis B virus (HBV) infection from a chronically infected sexual partner, the prevalence occult infections (OBIs) among donations partners HBV‐infected spouses and risk to safety remain poorly understood. Among 212 763 donors, 54 pairs (108 donations) were enrolled because one partner Several molecular serological examinations conducted....
Hepatitis B virus (HBV) infection is a major global health burden with 820 000 deaths per year. In our previous study, we found that the knockdown of autophagy-related protein 5 (ATG5) significantly upregulated interferon-stimulated genes (ISGs) expression to exert anti-HCV effect. However, regulation ATG5 on HBV replication and its underlying mechanism remains unclear. this screened altered type I interferon (IFN-I) pathway using RT² Profiler™ PCR array following knock-down bone marrow...
CRISPR-Cas technology is a widely utilized gene-editing tool that involves gRNA-guided sequence recognition and Cas nuclease-mediated cleavage. The design evaluation of gRNA are essential for enhancing CRISPR/Cas editing efficiency. Various assays such as single-strand annealing,
Abstract: Background : Cytidine/uridine monophosphate kinase 2 (UMP-CMP 2, CMPK2) has been reported as an antiviral interferon-stimulated gene (ISG). We previously observed that the expression of CMPK2 was significantly upregulated after Zika Virus (ZIKV) infection in A549 cells. However, role ZIKV replication remains to be determined. Methods or retinoic acid inducible I (RIG-I) overexpressed by plasmid transfection knocked down siRNA, respectively cells before were infected with ZIKV. RNA...