A5087041006- Cellular transport and secretion
- Connective tissue disorders research
- Congenital Diaphragmatic Hernia Studies
- Renal and related cancers
- Neonatal Respiratory Health Research
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Erythrocyte Function and Pathophysiology
- Bacterial Genetics and Biotechnology
- Epigenetics and DNA Methylation
- Lipid Membrane Structure and Behavior
- Vibrio bacteria research studies
- Wnt/β-catenin signaling in development and cancer
- Bacterial biofilms and quorum sensing
- Cancer Cells and Metastasis
- Microscopic Colitis
- Bacteriophages and microbial interactions
- Hippo pathway signaling and YAP/TAZ
- Salmonella and Campylobacter epidemiology
- Collagen: Extraction and Characterization
- Congenital heart defects research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Venomous Animal Envenomation and Studies
- Galectins and Cancer Biology
Stanford University
2018-2024
Centre for Genomic Regulation
2014-2018
Universitat Pompeu Fabra
2014-2018
Imperial College London
2012-2016
Medical Research Council
2016
Universidade do Porto
2013-2016
Instituto Gulbenkian de Ciência
2012
Cell stress and infection promote the formation of ubiquitinated aggregates in both non-immune immune cells. These structures are recognised by autophagy receptor p62/sequestosome 1 substrates for selective autophagy. The intracellular growth Salmonella enterica occurs a membranous compartment, Salmonella-containing vacuole (SCV), is dependent on effectors translocated to host cytoplasm pathogenicity island-2 (SPI-2) encoded type III secretion system (T3SS). Here, we show that bacterial...
Collagen export from the endoplasmic reticulum (ER) requires TANGO1, COPII coats, and retrograde fusion of ERGIC membranes. How do these components come together to produce a transport carrier commensurate with bulky cargo collagen? TANGO1 is known form ring that corrals we show here how this or fence assembled. Our data reveal organized by its radial interaction COPII, lateral interactions cTAGE5, TANGO1-short itself. Of particular interest finding recruits membranes for collagen via NRZ...
Procollagens, pre-chylomicrons, and pre–very low-density lipoproteins (pre-VLDLs) are too big to fit into conventional COPII-coated vesicles, so how these bulky cargoes exported from the endoplasmic reticulum (ER)? We have shown that TANGO1 located at ER exit site is necessary for procollagen export. report a role TANGO1-like (TALI), chimeric protein resulting fusion of MIA2 cTAGE5 gene products, in export pre-chylomicrons pre-VLDLs ER. binds TALI, both interact with apolipoprotein B (ApoB)...
Previously we showed that membrane fusion is required for TANGO1-dependent export of procollagen VII from the endoplasmic reticulum (ER) (<xref ref-type="bibr" rid="bib28">Nogueira, et al., 2014</xref>). Along with t-SNARE Syntaxin 18, now reveal complete complement SNAREs in this process, t-SNAREs BNIP1 and USE1, v-SNARE YKT6. TANGO1 recruits YKT6-containing ER Golgi Intermediate Compartment (ERGIC) membranes to VII-enriched patches on ER. Moreover residues 1214-1396, include...
TANGO1 binds and exports Procollagen VII from the endoplasmic reticulum (ER). In this study, we report a connection between cytoplasmic domain of SLY1, protein that is required for membrane fusion. Knockdown SLY1 by siRNA arrested in ER without affecting recruitment COPII components, general secretion, retrograde transport KDEL-containing BIP, ERGIC53. known to interact with ER-specific SNARE proteins Syntaxin 17 18, however only 18 was export. Neither nor export equally bulky I ER....
Autoinducer-2 (AI-2) a signal produced by range of phylogenetically distant microorganisms, enables inter-species cell-cell communication and regulates many bacterial phenotypes. Certain bacteria can interfere with AI-2-regulated behaviours neighbouring species internalizing AI-2 using the Lsr transport system (encoded lsr operon). imported is phosphorylated LsrK kinase AI-2-phosphate inducer operon. Here we show that in Escherichia coli phosphoenolpyruvate phosphotransferase (PTS) required...
How does mitosis influence the critical process of endocytosis? Some experiments lead to conclusion that endocytosis arrests completely during mitosis, whereas others indicate persists. We have resolved this apparent discrepancy by showing how conditions experiment its outcome. The dynamics clathrin-coated pit formation and uptake transferrin are maintained in naturally dividing cells but nearly absent mitotic arrested chemically treatment with nocodazole, S-Trityl-L-cysteine, or RO-3306....
Cell surface exposed cholesterol is critical for cell attachment and invasion of many viruses bacteria, including the bacterium Salmonella, which causes typhoid fever gastroenteritis. Using flow cytometry 3D confocal fluorescence microscopy, we found that mitotic cells - even though representing only 1–4% an exponentially growing population were much more efficiently targeted by Salmonella. This targeting was not dependent on spherical shape cells, but instead SipB- cholesterol-dependent....
ABSTRACT The distal lung contains terminal bronchioles and alveoli that facilitate gas exchange is affected by disorders including interstitial disease, cancer, SARS-CoV-2-associated COVID-19 pneumonia. Investigations of these localized pathologies have been hindered a lack 3D in vitro human culture systems. Further, stem cell identification has impaired quiescence, anatomic divergence from mouse lineage tracing clonogenic culture. Here, we developed robust feeder-free, chemically-defined...
Abstract Tissue-resident immunity underlies essential host defenses against pathogens, but analysis in humans has lacked vitro model systems where epithelial infection and accompanying resident immune cell responses can be observed en bloc. Indeed, human primary organoid cultures typically omit cells, tissue resident-memory lymphocytes are conventionally assayed without an component, for instance from peripheral blood, or after extraction organs. Further, the study of animals complicated by...
ABSTRACT Several bacterial pathogens and viruses interfere with the cell cycle of their host cells to enhance virulence. This is especially apparent in bacteria that colonize gut epithelium, where inhibition infected enhances intestinal colonization. We found intracellular Salmonella enterica serovar Typhimurium induced binucleation a large proportion epithelial by 14 h postinvasion effect was dependent on an intact pathogenicity island 2 (SPI-2) type 3 secretion system. The SPI-2 effectors...
This article was published in Mol Microbiol (2012) 84(1), 93–104. An error occurred where the wrong reference inadvertently cited with respect to trehalose measurements LB. Specifically sentence “Interestingly, is one of most abundant carbohydrates LB media (Xavier et al., 1995)”, which appeared second half first column on page 100 this paper wrong. should read: 1996)” and correct is: Xavier, K.B., Martins, L.O., Peist, R., Kossmann, M., Boos, W., Santos, H. (1996) High-affinity...