A5059946449- Cutaneous Melanoma Detection and Management
- Cutaneous lymphoproliferative disorders research
- Melanoma and MAPK Pathways
- Lymphoma Diagnosis and Treatment
- melanin and skin pigmentation
- Nonmelanoma Skin Cancer Studies
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Nail Diseases and Treatments
- Genetic and rare skin diseases.
- Autoimmune Bullous Skin Diseases
- Cellular Mechanics and Interactions
- Medicine and Dermatology Studies History
- Innovations in Medical Education
- Cancer and Skin Lesions
- Obesity, Physical Activity, Diet
- Skin Protection and Aging
- HER2/EGFR in Cancer Research
- Chronic Lymphocytic Leukemia Research
- Fungal Infections and Studies
- Monoclonal and Polyclonal Antibodies Research
- Oral Health Pathology and Treatment
- Allergic Rhinitis and Sensitization
- T-cell and Retrovirus Studies
- Cell Adhesion Molecules Research
Instituto de Salud Carlos III
2018-2025
Centro de Investigación Biomédica en Red de Cáncer
2018-2025
Hospital Universitari Sant Joan de Reus
2025
Universitat Rovira i Virgili
2025
Biomedical Research Institute of Lleida
2016-2025
Universitat de Lleida
2015-2025
Hospital Universitari Arnau de Vilanova
2016-2025
Kantonsspital Aarau
2025
Complejo Hospitalario Torrecárdenas
2003-2024
Universidad de Salamanca
2022
Cell migration is a key procedure involved in many biological processes including embryological development, tissue formation, immune defense or inflammation, and cancer progression. How physical, chemical, molecular aspects can affect cell motility challenge to understand migratory cells behavior. In vitro assays are excellent approaches extrapolate vivo situations study live Here we present four protocols that describe step-by-step migration, invasion adhesion strategies their...
ROCK-Myosin II drives fast rounded-amoeboid migration in cancer cells during metastatic dissemination. Analysis of human melanoma biopsies revealed that amoeboid with high Myosin activity are predominant the invasive fronts primary tumors proximity to CD206+CD163+ tumor-associated macrophages and vessels. Proteomic analysis shows controls an immunomodulatory secretome, enabling recruitment monocytes their differentiation into tumor-promoting macrophages. Both associated support abnormal...
The extracellular matrix (ECM) controls tumour dissemination. We characterise ECM organization in human and mouse tumours, identifying three regions: body, proximal invasive front distal front. Invasive areas show increased density, fibre thickness, length, alignment, with unique radial orientation at the correlating amoeboid features. Using patient samples murine models, we find that metastases recapitulate features of primary tumour. Ex vivo culture cancer cells isolated from different...
Cell migration underlies metastatic dissemination of cancer cells, and fast "amoeboid" in the invasive fronts tumors is controlled by high levels actomyosin contractility. How amoeboid regulated extracellular signals sustained over time transcriptional changes not fully understood. Transforming growth factor β (TGF-β) well known to promote epithelial-to-mesenchymal transition (EMT) contribute metastasis, but melanocytes are neural crest derivatives that have undergone EMT during embryonic...
Abstract Melanoma is a highly aggressive tumour that can metastasize very early in disease progression. Notably, melanoma disseminate using amoeboid invasive strategies. We show here high Myosin II activity, levels of ki-67 and tumour-initiating abilities are characteristic cells. Mechanistically, we find WNT11-FZD7-DAAM1 activates Rho-ROCK1/2-Myosin plays crucial role regulating potential, local invasion distant metastasis formation. Importantly, cells express both proliferative gene...
Abstract Cell migration is crucial for cancer dissemination. We find that AMP-activated protein kinase (AMPK) controls cell by acting as an adhesion sensing molecular hub. In 3-dimensional matrices, fast-migrating amoeboid cells exert low adhesion/low traction linked to ATP/AMP, leading AMPK activation. turn, plays a dual role controlling mitochondrial dynamics and cytoskeletal remodelling. High activity in adhering migratory cells, induces fission, resulting lower oxidative phosphorylation...
Metastasis involves dissemination of cancer cells away from a primary tumour and colonization at distal sites. During this process, the mechanical properties nucleus must be tuned since they pose challenge to negotiation physical constraints imposed by microenvironment tissue structure. We discovered increased expression inner nuclear membrane protein LAP1 in metastatic melanoma cells, invasive front human tumours metastases. Human express two isoforms (LAP1B LAP1C), which differ their amino...
We have studied a consecutive case series of patients with multiple primary melanoma (MPM) for the involvement susceptibility loci CDKN2A and CDK4.One hundred four MPM (81 two melanomas, 14 three, five four, one five, six, seven) were included.Seven different germline mutations identified in 17 (16.3%). In total, we 15 exon 2, 1alpha missense mutation, 1beta frameshift mutation. The age onset was significantly lower number melanomas higher mutations. more frequent familial history (35.5%)...
• Influence of clinicopathologic data on survival was analyzed in 43 patients with cutaneous T-cell lymphoma. The median age 66 years; 35 were male and eight female. extent the disease, established according to a modification TNM system, as follows: T1, three patients; T2, 15; T3,14; T4,11; N0, N1,28; M0, 38; M1,5; B0, 37; B1, six. first treatment applied after staging skin-limited therapy seven different regimens systemic chemotherapy 29. Seven received no or only topical corticosteroids...
We have recently reported that human melanoma cells express a variety of voltage-gated calcium (Ca(2+) ) channel types, including low-voltage-activated T-type channels play significant role in cell cycle progression. Here, we challenged metastatic with blockers clinical use and found dual effect on viability: (i) reduction the proliferation rate, through halt progression to G1 -S phase; (ii) promotion death was partially dependent activation caspases. An in-depth analysis process showed...
Background: Muir‐Torre syndrome (MTS) is characterized by the co‐existence of sebaceous gland tumors skin and internal malignancies. Currently, MTS regarded as a variant hereditary non‐polyposis colon cancer (HNPCC). Both HNPCC are secondary to germline mutations in DNA mismatch repair genes (mainly MSH‐2 MLH‐1). Methods: Cutaneous (eight adenomas, one carcinoma keratoacanthoma) (four colonic adenocarcinomas, two endometrial carcinomas, transitional cell carcinomas renal pelvis ureter,...
The expression of voltage-gated calcium channels (VGCCs) has not been reported previously in melanoma cells spite increasing evidence a role VGCCs tumorigenesis and tumour progression. To address this issue we have performed an extensive RT-PCR analysis VGCC human melanocytes range cell lines biopsies. In addition, tested the functional these using Ca(2+) imaging techniques examined their relevance for viability proliferation cells. Our results show that control express channel isoforms...
The Spanish National Cutaneous Melanoma Registry (Registro Nacional de Cutáneo [RNMC]) was created in 1997 to record the characteristics of melanoma at diagnosis. In this article, we describe these tumors diagnosis.This a cross-sectional observational study prevalent and incident cases for which initial biopsy results were available population-based RNMC.The RNMC contains information on 14,039 patients. We analyzed 13,628 melanomas diagnosed between 2011. total, 56.5% patients studied women...
We have recently demonstrated that proteasome inhibitors can be effective in inducing apoptotic cell death endometrial carcinoma lines and primary culture explants. Increasing evidence suggests reactive oxygen species are responsible for inhibitor-induced killing. Antioxidants thus block apoptosis (cell death) triggered by inhibition. Here, we evaluated the effects of different antioxidants (edaravone tiron) on cells treated with aldehyde (MG-132 or ALLN), boronic acid-based inhibitor...
The presence of at least one MC1R gene variant is associated with a reduction in age melanoma diagnosis families CDKN2A mutations.To describe dermoscopic features early mutation-positive Spanish individuals and to evaluate the possibility correlation between particular dermatoscopic pattern variants.Patients whom was diagnosed during specific follow up high-risk carrying mutations (with familial or personal history previous melanoma) were included this study. decision remove such melanomas...
The Spanish National Cutaneous Melanoma Registry (Registro Nacional de Cutáneo [RNMC]) was created in 1997 to record the characteristics of melanoma at diagnosis. In this article, we describe these tumors This a cross-sectional observational study prevalent and incident cases for which initial biopsy results were available population-based RNMC. RNMC contains information on 14,039 patients. We analyzed 13,628 melanomas diagnosed between 2011. total, 56.5% patients studied women 43.5% men....
Data regarding response to treatment in lymphomatoid papulosis (LyP) are scarce.To assess the daily clinical practice approach LyP and first-line treatments.This was a retrospective study enrolling 252 patients with LyP.Topical steroids, methotrexate phototherapy were most common treatments, prescribed for 35%, 20% 14% of patients, respectively. Complete (CR) achieved 48% treated patients. Eczematous lesions significantly increased relative risk (RR) not achieving CR (RR = 1.76; 95% CI...
Acral lentiginous melanoma (ALM) is a rare subtype primarily located in acral regions. However, ALMs exhibit distinctive genetic profile characterized by high number of copy variations (CNVs) and limited point mutations. Late diagnosis restricted therapeutic efficacy contribute to its poor prognosis. The secretome within the tumor microenvironment (TME) influences immune modulation plays vital role progression. We aim analyze ALM CNVs with prognosis primary patients. Here, we demonstrated...
Abstract The G101W founder mutation is the most common CDKN2A in Italy, Spain, and France. As background of modifying genes, environmental exposures, sun behavior vary across countries, studying carriers from distinct countries offers a unique opportunity to evaluate possible factors melanoma development. We evaluated 76 cases 59 carrier controls France, United States. Hair color dysplastic nevi distributions differed significantly 4 study groups. Cases also varied for eye color, freckling,...
Background Cutaneous malignant melanoma is an aggressive type of skin cancer which causes disproportionate mortality in young and middle-aged adults. Once disseminated, can be considered incurable disease, highly resistant to standard antineoplastic treatment, such as chemotherapy or radiation therapy. The proteasome represents a novel target for therapy that potentially used melanoma. Objectives To assess the effect four structurally different inhibitors on human cutaneous melanoma-derived...
Abstract Despite the use of multiple therapeutic strategies, metastatic melanoma remains a challenge for oncologists. Thus, new approaches using combinational treatment may be used to try improve prognosis this disease. In report, we have analyzed expression receptor tyrosine kinases (RTKs) in specimens and four cell lines. Both lines expressed RTKs, suggesting that they represent eventual targets multitargeted kinase inhibitor, Suntinib. Sunitinib reduced proliferation two (M16 M17)...