A5017814619- Ion Transport and Channel Regulation
- Neuroscience and Neuropharmacology Research
- Rheumatoid Arthritis Research and Therapies
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- HER2/EGFR in Cancer Research
- Bone Metabolism and Diseases
- Barrier Structure and Function Studies
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
- Signaling Pathways in Disease
- Cytokine Signaling Pathways and Interactions
- Ion Channels and Receptors
- Connexins and lens biology
- Bone health and treatments
- Galectins and Cancer Biology
- Systemic Lupus Erythematosus Research
- Neuroblastoma Research and Treatments
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- S100 Proteins and Annexins
- Mitochondrial Function and Pathology
- Protease and Inhibitor Mechanisms
- Nerve injury and regeneration
Nagoya City University
2013-2022
Mizuho (Japan)
1998-2021
Choju Medical Institute
2018
Fukushimura Hospital
2018
Teikyo University
2018
Nagoya Institute of Technology
2018
Kyoto University
2012-2014
McGill University
2009
Institute of Neurobiology and Molecular Medicine
2008
Japan Clinical Cancer Research Organization
2008
The K+ Cl- cotransporter KCC2 plays an important role in chloride homeostasis and neuronal responses mediated by ionotropic GABA glycine receptors. expression levels of neurons determine whether neurotransmitter are inhibitory or excitatory. is decreased developing neurons, as well response to various models injury epilepsy. We investigated there also direct modulation activity changes phosphorylation during such stressors. examined tyrosine rat hippocampal under different conditions vitro...
The membrane pore proteins, aquaporins (AQPs), facilitate the osmotically driven passage of water and, in some instances, small solutes. Under hyperosmotic conditions, expression AQPs changes, and studies have shown that AQP1 AQP5 is regulated by MAPKs. However, mechanisms regulating AQP4 AQP9 induced stress are poorly understood. In this study, we observed mannitol increased cultured rat astrocytes, intraperitoneal infusion brain cortex. addition, a p38 MAPK inhibitor, but not ERK JNK...
Abstract Interleukin (IL)‐1β is known to play a role in the formation of brain edema after various types injury. Aquaporin (AQP)4 also reported be involved progression edema. We tested hypothesis that AQP4 induced response IL‐1β. found expression mRNA and protein was significantly up‐regulated by IL‐1β cultured rat astrocytes, intracerebroventricular administration increased brain. The effects on induction were concentration time dependent. mediated through receptors because they abolished...
This study was conducted to determine changes in the expression of vascular endothelial growth factor (VEGF) murine retina after retinal scatter laser photocoagulation.Photocoagulation (PHC) performed on wild-type C57BL/6J mice using a diode laser, and eyes were enucleated 1, 2, 3, 4, 7, 14 days treatment. VEGF monocyte chemoattractant protein (MCP)-1 levels sensory pigmented epithelial (RPE) cells both tissues measured by ELISA. The mRNA real-time RT-PCR. Leukocyte infiltration into...
Ataxia telangiectasia (A-T) is a neurodegenerative disease caused by mutations in the large serine-threonine kinase ATM. A-T patients suffer from degeneration of cerebellum and show abnormal elevation serum alpha-fetoprotein. Here, we report novel signaling pathway that links ATM via cAMP-responsive-element-binding protein (CREB) to transcription factor ZFHX3 (also known as ATBF1), which turn promotes survival neurons inducing expression platelet-derived growth receptor β (PDGFRB). Notably,...
Abstract The hypoxia‐responsive cytokine erythropoietin (EPO) provides neuroprotective effects in the damaged brain during ischemic events and neurodegenerative diseases. purpose of present study is to evaluate EPO/EPO receptor (EPOR) endogenous system between astrocyte oligodendrocyte precursor cell (OPC) under hypoxia. We report here elevated EPO mRNA levels protein release cultured astrocytes following hypoxic stimulation by quantitative RT‐PCR ELISA. Furthermore, EPOR gene expressions...
The present study aimed to elucidate the function of AT motif-binding factor 1 (ATBF1) during neurogenesis in developing brain and primary cultures neuroepithelial cells cell lines (Neuro 2A P19 cells). Here, we show that ATBF1 is expressed differentiating field association with neuronal differentiation markers beta-tubulin MAP2 day E14.5 embryo rat brain, suggesting it promotes differentiation. In support this, suppresses nestin expression, a neural stem marker, activates promoter Neurod1...
Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinase (TIMPs) have been suggested to be involved in the pathogenesis asthma. Their expression airway smooth muscle (ASM) cells could collagen turnover migration these thus may contribute remodelling.To examine effect pro-fibrotic growth factors TGF-beta platelet-derived factor (PDGF) on MMPs/TIMPs cultured human ASM role MMP cells.ASM were stimulated with and/or PDGF. Expression activity MMP-1, MMP-2, MMP-3, TIMP-1 TIMP-2...
Cellular turnover of brain capillary endothelial cells (BCECs) by the balance cell proliferation and death is essential for maintaining homeostasis blood-brain barrier. Stimulation metabotropic ATP receptors (P2Y) transiently increased intracellular Ca²(+) concentration ([Ca²(+)](i)) in t-BBEC 117, a line derived from bovine BCECs. The [Ca²(+)](i) rise induced membrane hyperpolarization via activation apamin-sensitive small-conductance Ca²(+)-activated K(+) channels (SK2) enhanced 117. Here,...
Abstract: Hydraulic conductivity (L p ) of the plasma membrane Chara corallina was inhibited by HgCl 2 maximally about 95%. The inhibition reversed 2‐mercaptoethanol, reconfirming observation obtained Henzler and Steudle (1995). results suggest that osmotic water transport through cells occurs mostly via mercury‐sensitive channels containing thiol groups. ZnCl dissolved in APW (pH 5.6) also L 80% within 1–2 h, while Hepes‐Tris buffer 7.4) it 90% several minutes. Inhibition suggesting zinc...
Gliostatin is a polypeptide growth inhibitor of apparent M(r) = 100,000 with homodimeric structure comprising two 50-kDa subunits, acting on astrocyte as well astrocytoma cells (Asai, K., Hirano, T., Kaneko, S., Moriyama, A., Nakanishi, Isobe, I., Eksioglu, Y.Z., and Kato, T. (1992) J. Neurochem., 59, 307-317). The amino acid sequences 13 tryptic peptides including the terminus were completely identical to those platelet-derived endothelial cell factor (PD-ECGF) (Ishikawa, F., Miyazono,...
Perineural invasion is a prominent clinical feature of pancreatic cancer which causes difficulty in curative resection. In the present study, human cell lines, PaCa-2, AsPC-1, SW1990 and Capan-2, were all found to express abundant c-ret proto-oncogene mRNA RET protein, member receptor-tyrosine-kinase superfamily, identified as being receptor for glial-cell-line-derived neurotrophic factor (GDNF). an assay, migration cells was markedly induced by co-cultivation with glioma cells, T98G or...
Understanding the cellular and molecular changes in bone microenvironment is important for developing novel therapeutics to control breast cancer metastasis. Although underlying mechanism(s) of metastasis has been focus intense investigation, relatively little known about complex interactions between malignant cells stroma. Using a murine syngeneic model that mimics osteolytic associated with human cancer, we examined role tumor-bone interaction tumor-induced osteolysis growth...
Perinatal hypoxia-ischemia (HI) frequently causes white-matter injury, leading to severe neurological deficits and mortality, only limited therapeutic options exist. The white matter of animal models human patients with HI-induced brain injury contains increased numbers oligodendrocyte progenitor cells (OPCs). However, the origin fates these OPCs their potential repair injured remain unclear. Here, using cell-type- region-specific genetic labeling methods in a mouse HI model, we...