A5044328995- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Land Use and Ecosystem Services
- Environmental Impact and Sustainability
- Hedgehog Signaling Pathway Studies
- Advanced biosensing and bioanalysis techniques
- Hippo pathway signaling and YAP/TAZ
- Environmental Quality and Pollution
- Protist diversity and phylogeny
- Cancer-related molecular mechanisms research
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Remote Sensing and Land Use
- CRISPR and Genetic Engineering
- Geological and Geophysical Studies
- Wnt/β-catenin signaling in development and cancer
- Seismic Imaging and Inversion Techniques
- Extracellular vesicles in disease
- Chromosomal and Genetic Variations
- Aluminum Alloys Composites Properties
- Fetal and Pediatric Neurological Disorders
- Vehicle emissions and performance
- Metallurgy and Material Forming
- Biomedical Research and Pathophysiology
- Climate Change, Adaptation, Migration
Henan University
2025
China University of Mining and Technology
2010-2024
Qufu Normal University
2024
Lanzhou University
2022-2024
Army Medical University
2024
Southwest Hospital
2024
Model Animal Research Center
2024
Nanjing University
2024
Soochow University
2024
Second Affiliated Hospital of Soochow University
2024
Abstract Coring/logging data and physical property measurements from International Ocean Discovery Program Expedition 349 are integrated with, correlated to, reflection seismic to map sequence boundaries facies of the central basin neighboring regions South China Sea. First‐order interpreted, which Oligocene/Miocene, middle Miocene/late Miocene, Miocene/Pliocene, Pliocene/Pleistocene boundaries. A characteristic early Pleistocene strong reflector is also identified, marks top extensive...
PKD2 and PKD1 genes are mutated in human autosomal dominant polycystic kidney disease. can form either a homomeric cation channel or heteromeric complex with the receptor, presumed to respond ligand(s) and/or mechanical stimuli. Here, we identify two-residue hydrophobic gate PKD2L1, single-residue PKD2. We find that gain-of-function mutant effectively rescues knockdown-induced phenotypes embryonic zebrafish. The structure of activating F604P by cryo-electron microscopy reveals π- α-helix...
Osteogenic suppressors such as Sclerostin not only regulate skeletal development and regeneration but also serve anti-osteoporosis drug targets. However, very few druggable have been identified due to limited understanding of the molecular mechanisms governing osteogenesis. Here, we show that fibroblast activation protein (Fap), a serine protease inhibited by bone growth factor Osteolectin, is an osteogenic suppressor. Genetic deletion Fap significantly ameliorates limb trabecular loss...
Abstract Unlike adult mammalian heart, zebrafish heart has a remarkable capacity to regenerate after injury. Previous study shown Notch signaling activation in the endocardium is essential for regeneration of myocardium and this mediated by hemodynamic alteration injury, however, molecular mechanism not been fully explored. In we demonstrated that blood flow change could be perceived transmitted primary cilia dependent manner control responsive klf2 gene expression subsequent endocardium....
The highly conserved intraflagellar transport (IFT) proteins are essential for cilia formation in multiple organisms, but surprisingly, form zebrafish ift mutants. Here, we detected maternal deposition of gene products and found that ciliary assembly occurs only during early developmental stages, supporting the idea contribution masks function IFT initial development. In addition, basal bodies multiciliated cells pronephric duct mutants were disorganized, with a pattern suggestive defective...
Primary ciliary dyskinesia (PCD) is an autosomal recessive disease caused by defective cilia motility. The identified PCD genes account for about half of incidences and the underlying mechanisms remain poorly understood. We demonstrate that Reptin/Ruvbl2, a protein known to be involved in epigenetic transcriptional regulation, essential motility zebrafish. further show Reptin directly interacts with Lrrc6/Seahorse this interaction critical vivo function Moreover, whereas expression levels...
One major function of adipocytes is to store excess energy in the form triglycerides. Insufficient adipose lipid storage associated with many pathological conditions including hyperlipidemia, insulin resistance, and type 2 diabetes. In this study, we observed overexpression SUMO-specific protease (Senp2) tissues during obesity. Adipocyte Senp2 deficiency resulted less accompanied by an ectopic fat accumulation resistance under high-fat diet feeding. We further found that SET domain...
Phosphoinositides, a family of phosphorylated derivatives phosphatidylinositol (PtdIns), are tightly regulated both temporally and spatially by PtdIns phosphatases kinases. Mutations in inositol polyphosphate 5-phosphatase E ( INPP5E ) cause Joubert syndrome, human disorder associated with numerous ciliopathic defects, including renal cyst formation, linking phosphoinositides to ciliopathies. However, the molecular mechanism which INPP5E-mediated signaling regulates ciliogenesis cystogenesis...
Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism rarely been achieved. Here, we performed systematic all genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, high-throughput bioinformatics analysis, found that sequence...
P-TEFb activation by cAMP-PKA signaling promotes cystogenic gene transcription elongation and disease progression in ADPKD.
Cerebral ischemia-reperfusion injury (CIRI) is the main factor that leads to poor prognosis of cerebral ischemia. Apoptosis has been shown occur during process CIRI. Extracellular vesicles derived from mesenchymal stromal cells (MSCs-EVs) have broad potential for treating brain dysfunction and eliciting neuroprotective effects after stroke through neurogenesis angiogenesis. However, mechanism action extracellular CIRI not well known.A middle artery occlusion (MCAO) model was induced by...
Significance Both Hippo and c-Jun N-terminal kinase (JNK) signaling have well-established roles in promoting tissue growth, while it remains elusive whether how the two pathways interact or coordinate growth control. We report present study that Hpo–Yki pathway modulates Rho1–JNK signaling-mediated Drosophila wing development. show impaired Hpo activates JNK through transcriptional up-regulation of Rho1. These findings expands our understanding about cross-talk between important pathways,...
Transient receptor potential (TRP) channels, subdivided into 6 subfamilies in mammals, have essential roles sensory physiology. They respond to remarkably diverse stimuli, comprising thermal, chemical, and mechanical modalities, through opening or closing of channel gates. In this study, we systematically substituted the hydrophobic residues within distal fragment pore-lining helix S6 with hydrophilic and, based on Xenopus oocyte mammalian cell electrophysiology a gate theory, identified...
Although the CRISPR/Cas9 has been successfully applied in zebrafish, considerable variations efficiency have observed for different gRNAs. The workload and cost of zebrafish mutant screening is largely dependent on mutation rate injected embryos; therefore, selecting more effective gRNAs especially important construction. Besides sequence features, local chromatin structures may effects efficiency, which remain unexplored. In only related study nucleosome organization was not found to an...
There are about 180 villages in Mentougou District. The typical regional landscape characteristics and profound cultural historical geographical value of these ancient have rendered the district a unique tourism resource west Beijing. With ongoing social development population migration, values carried by faced with risk o f diminish ment. It is therefore great significance to integrate protect traditional promote this area. Based on 62 villages, paper uses concept linearity define spatial...
Many studies have proven that campus green space has healing effects, but there are few evaluation on the effects of a small-scale courtyard landscape high-density campus. This research comprehensively employs method semantic differential (SD method) and perceived restorative scale (PRS) to construct an framework based environmental preference in order quantify capability landscapes. The findings demonstrate following: (1) Plants close or higher than human visual height, such as trees...
The root system architecture (RSA) determines plant growth and yield. characterization of optimal RSA discovery genetic loci or candidate genes that control traits are therefore important research goals. However, the hidden nature makes it difficult to perform nondestructive, rapid analyses RSA. In this study, we developed an automated, high-throughput phenotyping platform (Root-HTP) a corresponding data processing pipeline for efficient, large-scale wheat (Triticum aestivum L.) This is...