A5071766653- Helicobacter pylori-related gastroenterology studies
- Burkholderia infections and melioidosis
- Antibiotic Resistance in Bacteria
- Mycobacterium research and diagnosis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Veterinary medicine and infectious diseases
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Gut microbiota and health
- Bone Tissue Engineering Materials
- Galectins and Cancer Biology
- Cystic Fibrosis Research Advances
- Clostridium difficile and Clostridium perfringens research
- Eosinophilic Esophagitis
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- IL-33, ST2, and ILC Pathways
- Graphene and Nanomaterials Applications
- Titanium Alloys Microstructure and Properties
- Metal and Thin Film Mechanics
- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Natural product bioactivities and synthesis
University of Malaya
2016-2025
University Malaya Medical Centre
2014-2024
University Kebangsaan Malaysia Medical Centre
2017
Hospital Kuala Lumpur
2014-2016
Faculty (United Kingdom)
2016
Imperial College London
2003
Wellcome Trust
2003
University of London
1986-1989
London School of Hygiene & Tropical Medicine
1986
University of Surrey
1986
Significance We demonstrate, to our knowledge for the first time, that bacterial biofilms are associated with colorectal cancers, one of leading malignancies in United States and abroad. Colon biofilms, dense communities bacteria encased a likely complex matrix contact colon epithelial cells, nearly universal on right tumors. Most remarkably, biofilm presence correlates tissue invasion changes biology enhanced cellular proliferation, basic feature oncogenic transformation occurring even...
Colorectal cancer (CRC) remains the third most common worldwide, with a growing incidence among young adults. Multiple studies have presented associations between gut microbiome and CRC, suggesting link risk. Although CRC continue to profile larger patient cohorts increasingly economical rapid DNA sequencing platforms, few been identified, in part due limitations taxonomic resolution differences analysis methodologies. Complementing these is newly recognized phenomenon that bacterial...
Background Accumulating evidence shows that Helicobacter pylori protects against some metabolic and immunological diseases in which the development of these coincide with temporal or permanent dysbiosis. The aim this study was to assess effect H. eradication on human gut microbiome. Methods As part currently on-going ESSAY (Eradication Study Stable Adults/Youths) study, we collected stool samples from 17 pylori-positive young adult (18–30 years-old) volunteers. same cohort followed up 6, 12...
Background Zingiber zerumbet Smith is a perennial herb, broadly distributed in many tropical areas. In Malaysia, it's locally known among the Malay people as "lempoyang" and its rhizomes, particularly, widely used traditional medicine for treatment of peptic ulcer disease beyond other gastric disorders. Aim study The aim current to evaluate gastroprotective effect zerumbone, main bioactive compound rhizome, against ethanol-induced model rats. Materials Methods Rats were pre-treated with...
Abstract Abnormal conduction and improper electrical impulse propagation are common in heart after myocardial infarction (MI). The scar tissue is non-conductive therefore the communication between adjacent cardiomyocytes disrupted. In current study, we synthesized characterized a conductive biodegradable scaffold by incorporating graphene oxide gold nanosheets (GO-Au) into clinically approved natural polymer chitosan (CS). Inclusion of GO-Au CS displayed two fold increase conductivity....
Herein, copper oxide nanoparticles (CuONPs) are proposed for widespread use in emerging biomedical applications.
Chromobacterium violaceum (C. violaceum) is a Gram-negative, rod-shaped facultatively anaerobic bacterium implicated with recalcitrant human infections. Here, we evaluated the anti-QS and antibiofilm activities of ethyl acetate extracts Passiflora edulis (P. edulis) on likely inactivation acyl-homoserine lactone (AHL)-regulated molecules in C. both by vitro silico analyses. Our investigations showed that sub-MIC levels were 2, 1, 0.5 mg/mL, concentrations marked reduction violacein pigment...
Human stomach is the only known natural habitat of Helicobacter pylori ( Hp ), a major bacterial pathogen that causes different gastroduodenal diseases. Despite this, impact on diversity and composition gastric microbiota has been poorly studied. In this study, we have analyzed culturable 215 Malaysian patients, including 131 positive 84 negative individuals were affected by Non- bacteria isolated from biopsy samples identified matrix assisted laser desorption ionization-time flight mass...
Abstract Photodynamic therapy (PDT) has emerged as a capable therapeutic modality for the treatment of cancer. PDT is targeted cancer that reportedly leads to tumor cell apoptosis and/or necrosis by facilitating secretion certain pro-inflammatory cytokines and expression multiple apoptotic mediators in microenvironment. In addition, also triggers oxidative stress directs killing activation inflammatory responses. However, cellular molecular mechanisms underlying role remain ambiguous. Here,...
Mitrella kentii (M. kentii) (Bl.) Miq, is a tree-climbing liana that belongs to the family Annonaceae. The plant rich with isoquinoline alkaloids, terpenylated dihydrochalcones and benzoic acids has been reported possess anti-inflammatory activity. purpose of this study assess gastroprotective effects desmosdumotin C (DES), new isolated bioactive compound from M. kentii, on gastric ulcer models in rats.
Helicobacter pylori is the etiological agent for diseases ranging from chronic gastritis and peptic ulcer disease to gastric adenocarcinoma primary B-cell lymphoma. Emergence of resistance antibiotics possesses a challenge effort eradicate H. using conventional antibiotic-based therapies. The molecular mechanisms that contribute these strains have yet be identified are important understanding evolutional pattern selective pressure imposed by environment.
Colorectal cancer (CRC) is ranked the third most common in human worldwide. However, exact mechanisms of CRC are not well established. Furthermore, there may be differences between Asian and Western populations. In present study, we utilized a liquid chromatography-mass spectrometry (LC-MS) metabolomic approach supported by 16S rRNA next-generation sequencing to investigate functional taxonomical paired tumor unaffected (normal) surgical biopsy tissues from 17 Malaysian patients. Metabolomic...
The genome of the human gastric pathogen Helicobacter pylori encodes a large number DNA methyltransferases (MTases), some which are shared among many strains, and others unique to given strain. MTases have potential roles in survival bacterium. In this study, we sequenced Malaysian H. clinical strain, designated UM032, by using combination PacBio Single Molecule, Real-Time (SMRT) Illumina MiSeq next generation sequencing platforms, used SMRT data characterize set methylated bases (the methylome).
Background Biofilm formation by Helicobacter pylori may be one of the factors influencing eradication outcome. However, genetic differences between good and poor biofilm forming strains have not been studied. Materials Methods yield 32 (standard strain 31 clinical strains) were determined crystal-violet assay grouped into poor, moderate groups. Whole genome sequencing these was performed on Illumina MiSeq platform. Annotation comparison genomic sequences carried out using RAST (Rapid...
In this study, 90 non-replicate imipenem-resistant Pseudomonas aeruginosa (IRPA) Malaysian isolates collected between October 2005 and March 2008 were subjected to a screening test for detection of the integron gene cassette. Class 1 integrons detected in 54 IRPA clinical isolates, whilst three contained class 2 integrons. Analysis cassettes associated with showed accC1 carrying bla(IMP-7) aacA7 bla(VIM-2). aadA6 was two bla(IMP-4). Using random amplification polymorphic DNA analysis, 14 PCR...
Abstract Back ground Helicobacter pylori infection is involved in several gastroduodenal diseases which can be cured by antimicrobial treatment. The aim of this study was to determine the prevalence H. and its bacterial resistance clarithromycin, fluoroquinolones, tetracycline Brazzaville, Congo, using molecular methods. Material Methods A cross‐ sectional carried out between September 2013 April 2014. Biopsy specimens were obtained from patients scheduled for an upper gastrointestinal...
Abstract Helicobacter pylori genetic diversity is known to be influenced by mobile genomic elements. Here we focused on prophages, the least characterized elements of H. . We present full sequences, insertion sites and phylogenetic analysis 28 prophages found in isolates from patients distinct disease types, ranging gastritis gastric cancer, geographic origins, covering most continents. The genome sizes these range 22.6–33.0 Kbp, consisting 27–39 open reading frames. A 36.6% GC was contrast...
Abstract Helicobacter pylori , is an invariably commensal resident of the gut microbiome associated with gastric ulcer in adults. In addition, these patients also suffered from a low grade inflammation that activates immune system and thus increased shunting energy to host defense mechanisms. To assess whether H. infection could affect growth early life, we determined expression levels selected metabolic hormones germ free (GF) specific pathogen-free (SPF) mice without presence . Despite...