Molecules Interacting with CasL (MICAL1), a multidomain flavoprotein monoxygenase, is strongly involved in the mechanisms that promote cancer cell proliferation and survival. Activation of MICAL1 causes an up-regulation reactive oxygen species (ROS) HeLa cells. ROS can function as signaling molecule modulates protein phosphorylation, leading to malignant phenotypes cells such invasion metastasis. Herein, we tested whether could control migration through regulating breast lines. The effects...

Abstract Molecule interacting with CasL 1 ( MICAL 1) is a multidomain flavoprotein mono‐oxygenase that strongly involves in cytoskeleton dynamics and cell oxidoreduction metabolism. Recently, results from our laboratory have shown modulates reactive oxygen species ROS ) production, the latter then activates phosphatidyl inositol 3‐kinase PI 3K)/protein kinase B (Akt) signalling pathway which regulates breast cancer invasion. Herein, we performed this study to assess involvement of...

CD24, a mucin-like membrane glycoprotein, plays critical role in carcinogenesis, but its human gastric cancer and the underlying mechanism remains undefined. The contents of CD24 epidermal growth factor receptor (EGFR) cells (SGC-7901 BGC-823) non-malignant epithelial (GES-1) were evaluated by Western blotting assay. Cellular EGFR staining was examined immunofluorescence Cell migration rate measured wound healing effects depletion/overexperssion on expression activation EGF/EGFR singaling...

// Yujie Zhang 1, 2, 3 , Jun Du Jianchao Zheng Jiaojing Liu 2 Rui Xu 4 Tian Shen Yichao Zhu Chang Hong Wang Zhihong 5 Fanqing Meng 6 Yan 7 Yongchang Chen 8 Yong 9 Luo Gu 1 Cancer Center, Nanjing Medical University, Nanjing, Jiangsu 210029, China Department of Biochemistry and Molecular Biology, Physiology, Biotechnology, Pathophysiology, the First Affiliated Hospital Drum Tower University School, 210008, Second 210011, School Science Laboratory Medicine, Zhenjiang, 212013, Correspondence to:...

Despite the fact that melatonin treatment shows some promise in gastric cancer, molecular mechanisms of cancer cells response to remains be determined.The SGC-7901 were treated with different concentrations for 24 and 48 h. Cell viability was determined by MTT assay, Hoechst 33258 staining FACS analysis used detect apoptotic cells. The contents activation apoptosis-related proteins HSP27, Akt P38 evaluated immunoblotting analysis. Then we HSP27-specific siRNA, PI3K inhibitor LY294002 or...

Aims and Hypothesis: NEDD9 is highly expressed in gastric cancer has a significant involvement its pathogenesis. However, the mechanism behind hypoxia-promoted cell migration regulation because of still unknown. The aim this study to investigate under hypoxia explore underlying potential molecular mechanisms.Cell motility was measured by wound healing transwell assay. MICAL1 expressions were examined western blot analysis. Interaction between assessed immunohistochemistry...

Tumor cell migration is a critical step in cancer metastasis. Over-activated Notch pathway can promote the of cells, especially breast cancer. However, underlying mechanism non-canonical signaling modulating has not yet been clearly characterized. Here we demonstrated that DAPT, gamma secretase inhibitor, inhibited protrusion formation and motility, then reduced triple-negative through increasing activity Cdc42 by pathway. Phosphorylation AKT on S473 was surprisingly increased when DAPT....

Hypoxia, a major condition associated with the tumor microenvironment, stimulates migration of cancer cells. SOX2 is powerful transcription factor that shows higher expression in several cancers, however, its role hypoxia-induced breast cell remains largely elusive.The human lines MDA-MB-231 and MDA-MB-468 were cultured under hypoxic conditions. The rate was determined using wound-healing transwell assays. protein levels SOX2, NEDD9 HIF-1α evaluated via western blotting analysis. mRNA qPCR....

Abstract Enhanced migration potential is a common characteristic of cancer cells induced by mechanisms that are incompletely defined. The present study was designed to investigate relationship new discovered cytoskeleton regulator MICAL‐L2 and the endogenous epidermal growth factor receptor (EGFR) signalling pathways in gastric cell migration. Increased expression up‐regulated EGFR protein level, accompanied increase migration, whereas silencing down‐regulated inhibited Expression also shown...

Epidermal growth factor (EGF) has been shown to induce the migration of various cancer cells. However, underlying signaling mechanisms for EGF-induced oral squamous cell carcinoma (OSCC) remain be elucidated. WNT7A, a member family 19 Wnt secreted glycoproteins, is commonly associated with tumor development. It mostly unknown whether and, if so, how EGF modulates WNT7A in OSCC The role was thus investigated explore OSCC.Cell measured by Wound healing assay and Transwell assay. Western...

Gestational diabetes mellitus (GDM) is one of the most prevalent metabolic diseases in pregnant women. In this study, we investigated effects Salusin-α rodent models GDM. We observed decreased levels placental tissue GDM mice. alleviated symptoms by reducing blood glucose and increasing serum insulin levels. Further analysis revealed that improved lipid profiles foetal outcomes Additionally, mitigated oxidative nitrosative stress mice enhancing Vitamin E, C, reduced GSH, while decreasing...

Melatonin, a naturally-occurring hormone, possesses antioxidant properties and ameliorates vascular endothelial dysfunction. In this study, we evaluate the impact of melatonin on migratory capability human umbilical vein cells (HUVECs) to hypoxia further investigate whether ERK/Rac1 signaling is involved in process. Here, found that inhibited hypoxia-stimulated hypoxia-inducible factor-1α (HIF-1α) expression cell migration dose-dependent manner. Mechanistically, Rac1 activation suppressed...

Arsenic trioxide (As₂O₃), a traditional remedy in Chinese medicine, has been used acute promyelocytic leukemia (APL) research and clinical treatment. Previous studies have shown that As₂O₃ exerts its potent antitumor effects solid tumors by regulating cell proliferation survival. The aim of this study was to investigate whether inhibited gastric cancer migration angiogenesis FOXO3a expression. We found reduced viability dose-dependent manner also vitro. Western blotting immunofluorescence...

GEP100, a guanine nucleotide exchanging factor (GEF) for Arf6, plays pivotal role in promoting breast cancer cell invasion both vitro and vivo. However, the precise mechanism GEP100-mediated is still poorly understood. In this study, we found that down-regulation of endogenous GEP100 MDA-MB-231 cells significantly inhibited EGF-induced invasion, which was rescued by over-expression ectopic GEP100. EGF increased Arf6 activity, ERK phosphorylation, uPAR expression time dependent manner....

Hypoxia-inducible factor 2α (HIF2α) plays critical roles in cancer progression. Although the mechanisms of HIF2α translation and degradation have been well studied, mechanism for regulation at transcriptional level is still not fully understood. Here, we present evidence that DNA methylation promoter contributes to transcription EPAS1 coding HIF2α. Methylated CpG binding protein 3 (MBD3) intricate regulatory mechanism. We showed MBD3 bound breast cells amplified through demethylating located...

Runt-related transcription factor 1 (RUNX1), an essential regulator of hematopoiesis, is overexpressed in patients with nonsmall-cell lung cancer (NSCLC) and correlated enhanced metastatic ability. Ras-interacting protein (Rasip1), a potential oncogene, required for blood vessel formation, recently, it has been shown that Rasip1 widely expressed NSCLC patients. We noticed promoter contains several RUNX1-binding sequences. However, the relationship between RUNX1 still unknown. In this study,...

E-cadherin internalization facilitates dissolution of adherens junctions and promotes tumor cell epithelial-mesenchymal transition (EMT) migration. Our previous results have shown that Arf6 exerts pro-migratory action in breast cancer cells after EGF stimulation. Despite the fact signaling stimulates EMT cells, effect on under treatment remains to be determined. Here, we showed dose-dependently stimulated by MCF-7 with maximal at 50 ng/ml. Meanwhile, markedly increased activation. was...

Aims and hypothesis: This study aims to investigate the mechanism involved in intracellualr regulation of EGFR degradation induced by EGF Methods: Phosphorylation proteins related signaling was examined western blot analysis. Activation, connection Rab35 interference with folliculin (FLCN) were assessed pulldown, coimmunoprecipitation assays separately. The relationship between FLCN cell growth detected using gene overexpression knock-down techniques. Results: FLCN, a tumor suppressor,...

Dynamic cytoskeletal rearrangements underlie the changes that occur during cell division in proliferating cells. MICAL2 has been reported to possess reactive oxygen species- (ROS-) generating properties and act as an important regulator of dynamics. However, whether it plays a role gastric cancer proliferation is not known. In present study, we found was highly expressed tissues, this high expression level associated with carcinogenesis poor overall survival patients. The knockdown led cycle...

Abstract Background Gastric cancer is a common and lethal human malignancy worldwide cell metastasis the leading cause of cancer-related mortality. MICAL2, flavoprotein monooxygenase, an important regulator epithelial-to-mesenchymal transition. The aim this study was to explore effects MICAL2 on gastric migration determine underlying molecular mechanisms. Methods Cell examined by wound healing transwell assays. Changes in E-cadherin/β-catenin signaling were determined qPCR analysis...

BACKGROUND:Few studies have examined functional brain changes specifically associated with chemotherapy (CTx) in patients lung cancer. This prospective longitudinal research aimed to explore the change intrinsic activity by investigating cancer after CTx. MATERIAL AND METHODS:Sixteen and 20 healthy individuals were enrolled this study. The amplitude of low-frequency fluctuation (ALFF), regional homogeneity (ReHo), dynamic (dALFF), (dReHo) computed. group differences resting state magnetic...