A5053310718- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Advanced Nanomaterials in Catalysis
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Microfluidic and Capillary Electrophoresis Applications
- Peptidase Inhibition and Analysis
- Cardiac Ischemia and Reperfusion
- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Extracellular vesicles in disease
- Graphene and Nanomaterials Applications
- Cardiac Structural Anomalies and Repair
- Pancreatic and Hepatic Oncology Research
- Cell Adhesion Molecules Research
- Nanoparticle-Based Drug Delivery
- Bee Products Chemical Analysis
- Neuropeptides and Animal Physiology
- Biotechnology and Related Fields
- Advancements in Transdermal Drug Delivery
- Tendon Structure and Treatment
- Steroid Chemistry and Biochemistry
University of Helsinki
2018-2024
Pharmaceutical Biotechnology (Czechia)
2019
Breast cancer, with around 2 million new cases in 2019, is the second most common cancer worldwide and leading cause of death among females. The aim this work to prepare a targeting nanoparticle through conjugation LinTT1 peptide, specific molecule p32 protein overexpressed by breast associated cells, on liposomes' surface. This approach increases cytotoxic effects doxorubicin (DOX) sorafenib (SRF) co-loaded therapeutic liposomes both 2D 3D cellular models. liposome functionalization leads...
Atherosclerosis still represents a major driver of cardiovascular diseases worldwide. Together with accumulation lipids in the plaque, inflammation is recognized as one key players formation and development atherosclerotic plaque. Systemic anti-inflammatory treatments are successful reducing disease burden, but correlated severe side effects, underlining need for targeted formulations. In this work, curcumin chosen payload model further loaded lignin-based nanoparticles (NPs). The NPs then...
Abstract The inability of the heart to recover from an ischemic insult leads formation fibrotic scar tissue and failure. From therapeutic strategies under investigation, cardiac regeneration holds promise restoring full functionality a damaged heart. Taking into consideration presence vast numbers fibroblasts myofibroblasts in injured heart, direct fibroblast reprogramming cardiomyocytes using small drug molecules is attractive option replenish lost cardiomyocytes. Here, spermine‐acetalated...
An alternative strategy of choosing photothermal and weak-immunostimulatory porous silicon@Au nanocomposites as particulate cores to prepare a biomimetic nanovaccine is reported improve its biosafety immunotherapeutic efficacy for solid tumors. A quantitative analysis method used calculate the loading amount cancer cell membranes onto nanocomposites. Assisted with foreign-body responses, these exogenous nanoparticulate weak immunostimulatory effect can still efficiently deliver into...
Fabrication of a dual-peptide functionalized acetalated dextran based nanosystem able to exploit macrophage recruitment occurring during myocardial infarction.
Tendinitis is a tendon disorder related to inflammation and pain, due an injury or overuse of the tissue, which hypocellular hypovascular, leading limited repair occurs in disorganized deposition extracellular matrix that leads scar formation fibrosis, ultimately resulting impaired integrity. Current conventional treatments are often ineffective, highlighting need for new therapeutic strategies. In this work, acetalated-dextran nanoparticles (AcDEX NPs) loaded with curcumin coated tannic...
Abstract Nanoparticle (NP)‐based intravenous administration represents the most convenient cardiac targeting delivery routine, yet, there are still therapeutic issues due to lack of efficiency and specificity. Active methods using functionalization ligands onto NPs’ surface may be limited by trivial modification procedures reduced yield in vivo. Here, a microfluidics assisted single step, green synthesis method is introduced for producing free heart homing NPs tailored manner. The generated...
Abstract Redox‐responsive silica drug delivery systems are synthesized by aeco‐friendly diatomite source to achieve on‐demand release of peptide nucleic acid (PNA) in tumor reducing microenvironment, aiming inhibit the immune checkpoint programmed cell death 1 receptor/programmed receptor ligand (PD‐1/PD‐L1) cancer cells. The nanoparticles (NPs) coated with polyethylene glycol chains as gatekeepers improve their physicochemical properties and control through cleavable disulfide bonds (S–S) a...
Here, we fabricated nanoparticles made solely from the membrane of cells found in pancreatic tumour's microenvironment (TME), like human MiaPaCa-2 and M2-polarized macrophages. The cell membrane-derived (CMNPs) deriving (MPC2-CMNPs) were loaded with chemotherapeutic drug paclitaxel (PTX), CMNPs macrophages (M2-CMNPs) colony-stimulating factor 1 receptor inhibitor, pexidartinib (PXDB). CMNPs' thorough morphological physicochemical characterisation was followed by an in-depth study their...
Recently, there has been an increasing interest for utilizing the host immune system to fight against cancer. Moreover, cancer vaccines, which can stimulate respond in long term, are being investigated as a promising approach induce tumor-specific immunity. In this work, we prepared effective vaccine (denoted "vacosome") by reconstructing cell membrane, monophosphoryl lipid A toll-like receptor 4 agonist, and egg phosphatidylcholine. The vacosome triggered enhanced bone marrow dendritic...
Abstract Myocardial infarction results in a massive loss of cardiomyocytes (CMs). Unfortunately, current therapies are unsuccessful replacing lost CMs, and thus, there is an urgent need for innovative approaches. Here, nanosystem based on spermine‐acetalated dextran (AcDXSp) encapsulating two drug compounds able to stimulate vitro CMs proliferation developed. The coated by deposition film constituted tannic acid (TA) Fe 3+ ions. coating with TA increases the retention nanocarrier cell...
Abstract Myocardial infarction (MI) is the leading cause of death worldwide. However, current therapies are unable to restore function injured myocardium. Advanced approaches, such as stimulation cardiomyocyte (CM) proliferation promising, but suffer from poor pharmacokinetics and possible systemic adverse effects. Nanomedicines can be a solution above‐mentioned drawbacks. targeting cardiac tissue still represents challenge. Herein, MI‐selective precision nanosystem developed, that relies on...
Abstract Nanotechnology holds the promise of bringing revolutionary therapeutic strategies into clinic. However, an enormous fraction currently proposed nanotechnology‐based therapies suffers from lack reproducibility, complexity, high costs, and scale‐up‐related issues. For these reasons, research community is moving toward miniaturization biomaterials fabrication methods. Customizable microfluidic‐based products have gained tremendous relevance in development biomedical technologies. This...
Abstract The analysis of nanoparticles’ biocompatibility and immunogenicity is mostly performed under a healthy condition. However, more clinically relevant evaluation conducted pathological condition less known. Here, the bio–nano interactions porous silicon nanoparticles (PSi NPs) are evaluated in an acute liver inflammation mice model. Interestingly, new mechanism which PSi NPs can remit hepatocellular damage activation surface dependent manner through protein corona formation, perturbs...
Microfluidics rose as an established, yet innovative technique thanks to the advantages brought by its dimension features. In article number 1800611, Flavia Fontana, João P. Martins, Giulia Torrieri, and Hélder A. Santos provide a tool kit for introduction microfluidics, together with selected examples of micro- nanoparticles production biomedical analyses.
Cardiac Repair In article number 2204666, Qiang Zhao, Xiaofeng Ye, Xianming Deng, Hélder A. Santos, and co-workers introduce a microfluidics-assisted single step, green synthesis method for producing targeting ligands free heart homing nanoparticles in tailored manner. The developed microfluidic platform facilitates an economically feasible simple yet robust procedure nanoparticle production, which shows great clinical translational potential as alternative post-conditioning routine after...
Nanovaccines A cancer cell membrane@porous silicon nanoparticles@Au nanovaccine is developed by Zhenyang Luo, Hélder A. Santos, Bing Xia, and co-workers in article number 2108012, based on weak-immunostimulatory porous silicon@Au nanoparticulate cores multi-antigenic membranes. These exogenous nanovaccines boost antitumor immunity, preventing the occurrence of solid tumors, significantly inhibiting growth metastasis tumors after photothermal therapy combined with immune checkpoint inhibitors.
The advance of nanomedicines has recently offered novel approaches to tackle cardiovascular diseases and, in particular, myocardial infarction (MI). However, the constant pumping heart and still poor knowledge targetable moieties, prevented application field rise. Tannic acid, a polyphenol derived from plants, showed affinity for components extracellular matrix, particular elastin, allowing retention protein aggregates cardiac tissue. Here, we explored targeting abilities tannic acid by...
Heart Targeting Nanoparticles In article number 2303658, Hélder A. Santos, Antti Saraste, Vimalkumar Balasubramanian, Giulia Torrieri, and co-workers develop a dual drug-loaded acetalated dextran-based precision nanosystem, that relies on the heart targeting properties of atrial natriuretic peptide (ANP) lin-TT1 peptide-mediated hitchhiking M2-like macrophages to target infarcted heart. These nanoparticles show increased at day 7 post-MI compared particles conjugated only with ANP.