A5010717285- Prostate Cancer Treatment and Research
- Natural product bioactivities and synthesis
- Cancer-related Molecular Pathways
- Retinoids in leukemia and cellular processes
- Genomics, phytochemicals, and oxidative stress
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Ginseng Biological Effects and Applications
- Estrogen and related hormone effects
- Cell death mechanisms and regulation
- Renal and related cancers
- Cancer, Lipids, and Metabolism
- Glutathione Transferases and Polymorphisms
- Sirtuins and Resveratrol in Medicine
- Microtubule and mitosis dynamics
- Cancer Treatment and Pharmacology
- Antioxidant Activity and Oxidative Stress
- Biological Activity of Diterpenoids and Biflavonoids
- Hormonal and reproductive studies
- Natural Compound Pharmacology Studies
- Sulfur Compounds in Biology
- Polysaccharides and Plant Cell Walls
- Cardiac Ischemia and Reperfusion
- Synthesis of Organic Compounds
- Toxoplasma gondii Research Studies
Kaohsiung Medical University
2015-2025
Kaohsiung Medical University Chung-Ho Memorial Hospital
2020-2025
Taipei Institute of Pathology
2015
Hospital Del Mar
2012
Municipal Institute for Medical Research
2012
Center for Environmental Health
2010
Institute of Biochemistry
2010
National Taiwan University
1995-2002
National Taiwan University Hospital
2000
Abstract Paclitaxel, one of the most commonly prescribed chemotherapeutic agents, is active against a wide spectrum human cancer. The mechanism its cytotoxicity, however, remains controversial. Our results indicate that paclitaxel treatment increases levels superoxide, hydrogen peroxide, nitric oxide (NO), oxidative DNA adducts, G2-M arrest, and cells with fragmented nuclei. Antioxidants pyruvate selenium, NO synthase inhibitor Nω-nitro-l-arginine methyl ester, scavenger manganese (III)...
Patients receiving docetaxel developed a drug resistance within few months. We generated docetaxel-resistant PC/DX25 and DU/DX50 CRPC cells from PC-3 DU-145 PCa cells, respectively. investigated the mechanism behind why exhibited higher migration invasion ability. Transwell assays were used to measure of cell. Fluorescence activated cell sorter (FACS) analysis was determine population cancer stem (CSC)-like Micro-Western Array (MWA) study changes protein profile. FACS revealed that contain...
Among many antioxidants that are used for the repairing of oxidative stress induced skin damages, we identified enriched astaxanthin extract (EAE) from Haematococcus pluvialis as a viable ingredient. EAE was extracted red microalgae through supercritical fluid carbon dioxide extraction. To compare effectiveness, wastreated on human dermal fibroblasts with other components, phorbol 12-myristate 13-acetate (PMA), and doxycycline. With sirius staining quantitative real-time polymerase chain...
Docetaxel has been approved by USFDA as a first-line treatment for castration-resistant prostate cancer (CRPC) patients. Patients receiving androgen deprivation therapy along with docetaxel result in superior survival, lower serum specific antigen (PSA) level, and better quality of life. However, significant proportion these patients ultimately develop resistance to within months. Caffeic acid phenethyl ester (CAPE), one the main bioactive components extracted from propolis, reported be...
Scope Kaempferol (KMP), a bioactive flavonoid compound found in fruits and vegetables, contributes to human health many ways but little is known about its relationship with muscle mass. The effect of KMP on C2C12 myoblast differentiation the mechanisms that might underlie are studied. Methods results This study finds (1, 10 µM) increases migration myoblasts vitro. Studying possible mechanism underlying migration, activates Integrin Subunit Beta 1 (ITGB1) myoblasts, increasing p‐FAK (Tyr398)...
Abstract BACKGROUND The modulatory effects and molecular mechanisms of curcumin (CCM) on the cytotoxicity chemotherapeutic agents to prostate cancer cells were explored. METHODS combined CCM examined by three different administration schedules (one concurrent two sequential treatments) in androgen‐independent (AIPC) (PC‐3 DU145). Alteration cell cycle progression, protein levels, transcriptional activation PC‐3 assayed flow cytometry, Western blotting, gel shift assay, respectively. RESULTS...
An elevated level of IL-10 has been considered a critical factor for the development endometriosis; however, its detailed mechanism and causal relationship remain unclear. This study explored cellular source angiogenic activity local during early stage endometriosis. Using surgical murine model, we found that localised treatment with exogenous recombinant on day surgery significantly enhanced endometriotic lesion growth angiogenesis, whereas blocking using mAbs suppressed those effects....
Epidermal growth factor receptor (EGFR) is upregulated in prostate cancer (PCa). However, suppression of EGFR did not improve the patient outcome, possibly due to activation PI3K/Akt signaling PCa. Compounds able suppress both and may be effective for treating advanced We examined if caffeic acid phenethyl ester (CAPE) simultaneously suppresses Akt signaling, migration tumor PCa cells. Wound healing assay, transwell assay xenograft mice model were used determine effects CAPE on proliferation...
Interleukin (IL)-6-mediated anti-apoptotic effects and drug-resistance mechanisms in prostate cancer cells were investigated.IL-6 levels of PC-3 LNCaP studied by using ELISA. Protective IL-6 on cytotoxic agent-induced apoptosis exogenous serum-starved anti-sense strategy. Western blotting reverse transcription-polymerase chain reaction (RT-PCR) used to determine Bcl-2 family proteins. Tetracycline-regulated Bcl-xL expression system dominant negative STAT3 transfectants study signaling...
The combined effects of ling-zhi polysaccharide fraction 3 (LZP-F3) and anticancer drugs (cisplatin arsenic trioxide) were examined in three human urothelial carcinoma (UC) cells (parental, NTUB1; cisplatin-resistant, N/P(14); arsenic-resistant, N/As(0.5)). MTT assay median-effect analysis revealed that LZP-F3 could profoundly reverse the chemosensitivity N/P(14) N/As(0.5) to cisplatin arsenic, respectively, a dose-dependent manner, which involved activation p38 down-regulation Akt XPA. A...
ABSTRACT Transcription factor CCAAT/enhancer‐binding protein delta (CEBPD) plays multiple roles in tumor progression. Studies have demonstrated that cisplatin (CDDP) induced CEBPD expression and had led to chemotherapeutic drug resistance. However, the underlying molecular mechanisms of CDDP‐regulated its relevant CDDP responses remain elusive. MicroRNAs (miRNAs) are small non‐coding RNAs negatively regulate gene a sequence‐specific manner. Abnormal miRNAs is associated with In current...
Metastatic renal cell carcinoma (RCC) is highly resistant to conventional systemic treatments, including chemotherapy, radiotherapy and hormonal therapies. Previous studies have shown over-expression of EGFR associated with high grade tumors a worse prognosis. Recent suggest anticancer therapies targeting the pathway promising results in clinical trials RCC patients. Therefore, characterization level localization expression important for target-dependent therapy. In this study, we...
Caffeic acid phenethyl ester (CAPE) is the main bioactive component of poplar type propolis. We previously reported that treatment with caffeic suppressed cell proliferation, tumor growth, as well migration and invasion prostate cancer (PCa) cells via inhibition signaling pathways AKT, c-Myc, Wnt EGFR. also demonstrated combined CAPE docetaxel altered genes involved in glycolysis tricarboxylic (TCA) cycle. therefore suspect may interfere glucose metabolism PCa cells. Seahorse Bioenergetics...
Background Alzheimer's disease (AD) is the main cause of dementia in an aging society. Previous studies have demonstrated that non-invasive light flicker and sound with gamma frequency oscillations can modulate AD-related pathology AD mice, potentially improving patient outcomes. However, molecular mechanism by which inhibits expression amyloid-β 1–42 (Aβ 42 ) phosphorylation tau, modulating cell autophagy nerve cells are still unclear. Objective This study aimed to explore effects 40 Hz...