A5047608626- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Genomics and Chromatin Dynamics
- Prenatal Screening and Diagnostics
- RNA and protein synthesis mechanisms
- Reproductive Biology and Fertility
- Parvovirus B19 Infection Studies
- Birth, Development, and Health
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neurogenetic and Muscular Disorders Research
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Fungal and yeast genetics research
- Biotin and Related Studies
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- Nuclear Structure and Function
China Agricultural University
2025
Capital Normal University
2023-2024
Yangzhou University
2024
University of Pennsylvania
2018-2024
California University of Pennsylvania
2024
Yancheng Second People's Hospital
2020-2024
University of California, Los Angeles
2023
First Affiliated Hospital of Xi'an Jiaotong University
2021
The University of Western Australia
2016
SKP1 coordinates chromosome synapsis and meiotic recombination with progression through the first cell division in mouse.
Abstract Homeostasis of meiotic DNA double strand breaks (DSB) is critical for germline genome integrity and homologous recombination. Here we demonstrate an essential role SKP1, a constitutive subunit the SCF (SKP1-Cullin-F-box) ubiquitin E3 ligase, in early processes. SKP1 restrains accumulation HORMAD1 pre-DSB complex (IHO1-REC114-MEI4) on chromosome axis germ cells. Loss prior to meiosis leads aberrant localization DSB repair proteins failure synapsis initiation both males females....
Abstract The molecular regulatory mechanisms of host responses to Mycobacterium avium subsp. paratuberculosis (MAP) infection during the early subclinical stage are still not clear. In this study, surgically isolated ileal segments in newborn calves (n = 5) were used establish vivo MAP adjacent an uninfected control intestinal compartment. RNA-Seq was profile whole transcriptome (mRNAs) and microRNAome (miRNAs) tissues collected at one-month post-infection. most related function...
Meiotic progression requires coordinated assembly and disassembly of protein complexes involved in chromosome synapsis meiotic recombination. Mouse TRIP13 its ortholog Pch2 are instrumental remodeling HORMA domain proteins. HORMAD proteins associated with unsynapsed axes but depleted from the synaptonemal complex (SC) synapsed homologs. Here we report that localizes to SC early pachytene spermatocytes telomeres throughout prophase I. Loss leads arrest thus sterility both sexes. Trip13 -null...
Abstract Proper nutrition is essential for sustaining optimal reproductive function in both humans and ruminants. Studies have shown that undernutrition overnutrition can negatively impact human processes. However, the Mediterranean diet, with its balanced nutrient‐rich composition, recognised positive effects on reproduction. In context of ruminants, a well‐balanced diet equally crucial achieving peak performance. Grazing, which allows ruminants to feed natural pasture open environments,...
Our aim was to evaluate the clinical utility of non-invasive prenatal testing for pregnant women with different diagnostic indications.In eight counties and districts Yancheng, we studied 13,149 indications who were offered NIPT fetal screening, including sex chromosomal aneuploidies (SCAs), rare autosomal trisomies (RATs), subchromosomal copy number variations (CNVs). The purpose compare detection positive predictive values (PPVs) use NIPT. results validated by karyotyping, microarray...
Objective: Pregnancy-associated plasma protein-A2 (PAPP-A2) is the homolog of PAPP-A in vertebrate genome and its role protecting against salt-induced hypertension salt-sensitive rats has been confirmed. We sought to examine associations PAPP-A2 levels genetic variants with salt sensitivity, blood pressure (BP) changes incidence humans. Methods: Eighty participants (18–65 years old) sequentially consuming a usual diet, 7-day low-salt diet (3.0 g/day) high-salt (18 g/day). In addition, we...
Transposable elements constitute nearly half of the mammalian genome and play important roles in evolution. While a multitude both transcriptional post-transcriptional mechanisms exist to silence transposable elements, control transposition vivo remains poorly understood. MOV10, an RNA helicase, is inhibitor mobilization retrotransposons retroviruses cell culture assays. Here we report that MOV10 restricts LINE1 retrotransposition mice. Although broadly expressed, its loss causes only...
In brief The dissociation of HORMA domain protein 2 (HORMAD2) from the synaptonemal complex is tightly regulated. This study reveals that N-terminal region HORMAD2 critical for its synapsed meiotic chromosomes. Abstract During meiosis, homologous chromosomes undergo synapsis and recombination. proteins regulate key processes in meiosis. Mammalian HORMAD1 localize to unsynapsed chromosome axes but are removed upon by TRIP13 AAA+ ATPase. engages induce an open conformation, resulting...
Abstract Germ cell development depends on the capacity of somatic Sertoli cells to undergo differentiation into a mature state and establish germ cell‐specific blood–testis barrier (BTB). The BTB structure confers an immunological for meiotic postmeiotic cells, its dynamic permeability facilitates transient movement preleptotene spermatocytes through enter meiosis. However, regulatory factors involved in maturation how dynamics coordinate remain unclear. Here, we found histone deacetylase...
Meiotic progression requires coordinated assembly and disassembly of protein complexes involved in chromosome synapsis meiotic recombination. The AAA+ ATPase TRIP13 its orthologue Pch2 are instrumental remodeling HORMA domain proteins. Meiosis-specific HORMAD proteins associated with unsynapsed axes but depleted from the synaptonemal complex (SC) synapsed homologues. Here we report that localizes to SC early pachytene spermatocytes telomeres throughout prophase I. Loss leads arrest thus...
Meiotic progression requires coordinated assembly and disassembly of protein complexes involved in chromosome synapsis meiotic recombination. Mouse TRIP13 its ortholog Pch2 are instrumental remodeling HORMA domain proteins. HORMAD proteins associated with unsynapsed axes but depleted from the synaptonemal complex (SC) synapsed homologs. Here we report that localizes to SC early pachytene spermatocytes telomeres throughout prophase I. Loss leads arrest thus sterility both sexes. Trip13 -null...
Piwi-interacting RNAs (piRNAs) play critical roles in protecting germline genome integrity and promoting normal spermiogenic differentiation. In mammals, there are two populations of piRNAs: pre-pachytene pachytene. Transposon-rich piRNAs expressed fetal perinatal germ cells required for retrotransposon silencing, whereas transposon-poor pachytene spermatocytes round spermatids regulate mRNA transcript levels. MOV10L1, a cell-specific RNA helicase, is essential the production both piRNAs....
The microrchidia (MORC) family proteins are chromatin-remodelling factors and function in diverse biological processes such as DNA damage response transposon silencing. Here, we report that mouse Morc2b encodes a functional germ cell-specific member of the MORC protein family. arose specifically rodent lineage through retrotransposition Morc2a during evolution. Inactivation leads to meiotic arrest sterility both sexes. Morc2b-deficient spermatocytes oocytes exhibit failures chromosomal...
Abstract Meiotic progression requires coordinated assembly and disassembly of protein complexes involved in chromosome synapsis meiotic recombination. The AAA+ ATPase TRIP13 its orthologue Pch2 are instrumental remodeling HORMA domain proteins. Meiosis-specific HORMAD proteins associated with unsynapsed axes but depleted from the synaptonemal complex (SC) synapsed homologues. Here we report that localizes to SC early pachytene spermatocytes telomeres throughout prophase I. Loss leads arrest...
Chromosome microarray analysis (CMA) and whole exome sequencing (WES) are increasingly utilized in prenatal diagnosis of abnormal ultrasound findings, but studies on correlation between pathogenic copy number variations (pCNVs) single-gene mutations fetuses with nuchal translucency (NT) thickening/cystic hygroma (CH), pregnancy outcomes, rare. This study aimed to investigate clinical value CMA WES for NT thickening/CH fetuses, explore genetic fetal thickening CH, analyze outcomes. We...