A5083763196- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Infectious Diseases and Mycology
- Pneumocystis jirovecii pneumonia detection and treatment
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Diagnosis and treatment of tuberculosis
- Gut microbiota and health
- Essential Oils and Antimicrobial Activity
- vaccines and immunoinformatics approaches
- Clostridium difficile and Clostridium perfringens research
- SARS-CoV-2 and COVID-19 Research
- Genomics and Phylogenetic Studies
- Phytochemistry and biological activities of Ficus species
- Zoonotic diseases and public health
- Animal Disease Management and Epidemiology
- Antimicrobial Resistance in Staphylococcus
- Ethnobotanical and Medicinal Plants Studies
- Vibrio bacteria research studies
- Plant Pathogens and Fungal Diseases
- HIV/AIDS oral health manifestations
- Hibiscus Plant Research Studies
- Dental Research and COVID-19
Noguchi Memorial Institute for Medical Research
2016-2025
University of Ghana
2016-2025
MRC Unit the Gambia
2024
Rush University Medical Center
2022
Human tuberculosis (TB) is caused by members of the Mycobacterium complex (MTBC). The MTBC comprises several human-adapted lineages known as M. sensu stricto, well two (L5 and L6) traditionally referred to africanum. Strains L5 L6 are largely limited West Africa for reasons unknown, little their genomic diversity, phylogeography evolution. Here, we analysed genomes 350 320 strains, isolated from patients 21 African countries, plus 5 related that had not been classified into any lineages. Our...
Mycobacterium africanum is a member of the tuberculosis complex (MTBC) and an important cause human in West Africa that rarely observed elsewhere. Here we genotyped 613 MTBC clinical isolates from Ghana, searched for associations between different phylogenetic lineages patient variables. We found 17.1% (105/613) belonged to M. africanum, with remaining belonging sensu stricto. No bovis was identified this sample. significantly more common patients Ewe ethnic group (adjusted odds ratio: 3.02;...
Tuberculosis (TB) caused by Mycobacterium tuberculosis remains an ongoing threat to human health. Several medicinal plants are used traditionally treat in Ghana. The current study was designed investigate the antimycobacterial activity and cytotoxicity of crude extracts from five selected plants.The microplate alamar blue assay (MABA) for studies while CellTiter 96® AQueous Assay, which is composed solutions a novel tetrazolium compound...
Background There is a perception that genomic differences in the species/lineages of nine species making Mycobacterium tuberculosis complex (MTBC) may affect efficacy distinct control tools certain geographical areas. We therefore analyzed prevalence and spatial distribution MTBC lineages among isolates from pulmonary TB cases over an 8-year period, 2007–2014. Methodology Mycobacterial isolated by culture consecutively recruited patients presenting at selected district/sub-district health...
Mycobacterium africanum comprises two phylogenetic lineages within the M. tuberculosis complex (MTBC) and is an important cause of human (TB) in West Africa. The reasons for this geographic restriction remain unclear. Here, we performed a prospective study to explore associations between characteristics TB patients MTBC circulating Ghana.We genotyped 1,211 isolates recovered from pulmonary recruited 2012 2014 using single nucleotide polymorphism typing spoligotyping. Associations patient...
We spoligotyped and screened 1490 clinical Mycobacterium tuberculosis complex strains from Northern Greater Accra regions of Ghana against INH RIF using the microplate alamar blue phenotypic assay. Specific drug resistance associated genetic elements resistant were analyzed for mutations. A total 111 (7.5%), 10 (0.7%) 40 (2.6%) mono-resistant to INH, RIF, MDR, respectively. found spoligotype be with (INH: 22.1%; p = 0.0000, RIF: 6.2%; 0.0103, MDR: 4.6%; 0.0240) as compared Cameroon 6.7%,...
Abstract Mycobacterium africanum ( Maf) causes a substantial proportion of human tuberculosis in some countries West Africa, but little is known on this pathogen. We compared the genomes 253 Maf clinical isolates from Ghana, including N = 175 Lineage 5 (L5) and 78 6 (L6). found that genomic diversity L6 was higher than L5 despite smaller sample size. Regulatory proteins appeared to evolve neutrally under purifying selection L6. Even though over 90% T cell epitopes were conserved both...
ObjectiveUnderstanding transmission dynamics is useful for tuberculosis (TB) control. A population-based molecular epidemiological study was conducted to determine TB in Ghana.MethodsMycobacterium complex (MTBC) isolates obtained from prospectively sampled pulmonary patients between July 2012 and December 2015 were characterized using spoligotyping standard 15-locus mycobacterial interspersed repetitive unit variable number tandem repeat (MIRU-VNTR) typing studies.ResultsOut of 2309 MTBC...
Background The epidemiology of Mycobacterium tuberculosis complex (MTBC) lineage 5 (L5) infections in Ghana revealed a significantly increased prevalence Ewes compared to other self-reported ethnic groups. In that context, we sought investigate the early phase (TB) infection using ex vivo macrophages derived from blood Ewe and Akan group volunteers with MTBC L4 L5 strains. Methods study participants consisted 16 controls, among which ethnicity was equally represented, as well 20 cured TB...
We have previously shown that secondary infections of Buruli ulcer wounds were frequently caused by Staphylococcus aureus. To gain understanding into possible routes infection, we characterized S. aureus isolates from patient lesions and surrounding environments across two Ghanaian health centres. One hundred one isolated (n = 93, 92.1%) the hospital environment 8, 7.9%) spa gene, mecA Panton-Valentine leucocidin toxin followed sequencing whole genome a subset 49 isolates. Spa typing gene 91...
Background Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a re-emerging problem in both livestock and humans. The association of some M. strains with hyper-virulence, MDR-TB disseminated disease makes it imperative to understand the biology pathogen. Methods (15) among 1755 complex (MTBC) isolated between 2012 2014 were characterized analyzed for associated patient demography other risk factors. Five isolates whole-genome sequenced comparatively against global collection...
The confirmed case fatality rate for the coronavirus disease 2019 (COVID-19) in Ghana has dropped from a peak of 2% March to be consistently below 1% since May 2020. Globally, rates have been linked strains/clades circulating severe acute respiratory syndrome 2 (SARS-CoV-2) within specific country. Here we present 46 whole genomes SARS-CoV-2 Ghana, two separate sequencing batches: 15 isolates early epidemic (March 12–April 1 2020) and 31 later time-points ( 25–27 2020). Sequencing was...
Whole genome sequencing (WGS) is progressively being used to investigate the transmission dynamics of Mycobacterium tuberculosis complex (MTBC). We WGS analysis resolve traditional genotype clusters and explored spatial distribution confirmed recent clusters. Bacterial genomes from a total 452 MTBC isolates belonging large population-based study spanning July 2012 December 2015 were obtained through short read next-generation using illumina HiSeq2500 platform. performed clustering specified...
Rapid but simple diagnostic tools for the detection of drug-resistant (DR) tuberculosis (TB) have been acknowledged as being important its effective management and control.To establish a molecular line-probe assay (GenoType MTBDRplus) detecting DR-TB in Ghana.We first screened 113 Mycobacterium isolates using indirect proportion method MTBDRplus. The rpoB katG genes promoter regions oxyR-ahpC inhA were sequenced to identify mutations found be resistant on phenotypic drug susceptibility...
Objective/Background: Nontuberculous mycobacterial (NTM) species are assuming public health importance in pulmonary diseases; they increasingly being isolated, and importantly, most NTMs do not respond to routine tuberculosis (TB) drugs. This study aimed identify isolated from TB cases also determine their susceptibility streptomycin (STR), isoniazid (INH), rifampicin (RIF). Methods: A total of 1755 isolates, obtained between August 2012 July 2014, 2036 smear-positive were identified using...
This study aimed to isolate nontuberculous mycobacterial species from environmental samples obtained some selected communities in Ghana. To optimize decontamination, spiked were used evaluate four decontamination solutions and supplemented media, after which the best solution media for actual analysis. The isolates identified on basis of specific genetic sequences, including heat shock protein 65, IS2404, IS2606, rpoB, ketoreductase gene, as needed. Among methods evaluated, with 1 M NaOH...
Cholera remains a major global public health threat and continuous emergence of new Vibrio cholerae strains is concern. We conducted molecular epidemiological study to detect virulence markers antimicrobial resistance patterns V. isolates obtained from the 2012-2015 cholera outbreaks in Ghana. Archived clinical 2012, 2014 2015 Ghana were revived by culture subjected microscopy, biochemical identification, serotyping, antibiotic susceptibility testing, detection distinct factors Multi-Locus...
Tuberculosis (TB), caused by Mycobacterium tuberculosis complex (MTBC), remains a global health burden, claiming millions of lives annually. Despite the availability vaccine (the Bacillus Calmette-Guérin; BCG), diagnostics (smear microscopy and DNA-based diagnostics) drugs for treatment, challenges like drug resistance, lower diagnostic sensitivity such as microscopy, instrumentation high-level operational expertise requirement poor efficacy pose greater hinderance to TB monitoring control....
Buruli ulcer (BU) is a subcutaneous skin disease listed among the neglected tropical diseases by World Health Organization (WHO). Early case detection and management very important to reduce morbidity accompanied characteristic disfiguring nature of BU. Since diagnosis based on clinical evidence can lead misdiagnosis, microbiological confirmation essential abuse drugs; since anti-mycobacterial drugs are also used for TB treatment. The current WHO gold standard PCR method expensive, requires...
Differentiation of the Mycobacterium tuberculosis complex (MTBc) from non-tuberculous mycobacteria (NTM) is important for diagnosis and a prerequisite reliable phenotypic drug-resistance testing. We evaluated performance rapid MPT64 antigen identification test detection africanum lineage 5 (MAF L5).Smear-positive patients' sputa were included prospectively. Culture was performed on Löwenstein-Jensen medium and, when positive, classical para-nitro benzoic acid susceptibility heat-labile...