Cerebral infarction ranks as the second leading cause of disability and death globally, inflammatory response glial cells is main brain damage during cerebral infarction.Studies have shown that mesenchymal stem (MSCs) can secrete exosomes contribute to disease. Here, we would explore function MSC-derived exosome in infarction.Microarray indicated a decrease miR-542-3p an increase Toll-Like Receptor 4 (TLR4) middle artery occlusion (MCAO) mice comparing with sham mice. And luciferase RIP...

Microglia are the resident immune cells in central nervous system and play an essential role brain homeostasis neuroprotection diseases. Exosomes crucial intercellular communication by transporting bioactive miRNAs. Thus, this study aimed to investigate function of microglial exosome presence ischemic injury related mechanism. Oxygen-glucose deprivation (OGD)-treated neurons transient middle cerebral artery occlusion (TMCAO)-treated mice were applied study. Western blotting, RT-PCR, RNA-seq,...

Background: Interleukin-1β (IL-1)-treated mesenchymal stem cells (MSCs) and IL-1-MSCs-conditioned medium (CM) exert anti-inflammatory roles. Astrocytes are essential for the modulation of synaptic activity neuronal homeostasis in brain. Exosomes critical mediators intercellular communication. However, mechanism underlying effect IL-1-treated MSCs remains unknown. Methods: In this study, exosomes (IL-1-Exo) were isolated from MSCs. addition, lipopolysaccharide (LPS)-treated hippocampal...

Osteoarthritis (OA) is one of the most common diseases affecting older people; however, there remains no effective targeted drug to combat OA. The aims this study were (1) explore effect T140 in regulating degeneration articular cartilage vivo by blocking SDF-1/CXCR4 signaling pathway, and (2) provide experimental evidence for development a novel OA-targeted pharmacotherapy. Thirty-six healthy Hartley guinea pigs randomly divided into three groups: T140-treated group (n = 12), phosphate...

Abstract The oxidative chemical polymerization of 3, 4‐ethylenedioxythiophene (EDOT) was conducted at room temperature in the presence poly(styrene sulfonate) (PSS) as doping agent, sodium supersulphate (Na 2 S O 8 ) and ferric sulphate(Fe (SO 4 3 compound oxidant deionized water solvent. In order to remove ion + ), (Fe 3+ sulfate (SO4 2− certain amount exchanger added after 24 h, dark blue poly(3, 4‐ethylenedioxythiophene) (PEDT)/PSS solution obtained. influence different proportions EDOT...

Osteoarthritis (OA) is an aseptic inflammatory disease which associated with the stromal cell‑derived factor 1/C‑X‑C chemokine receptor type 4 (SDF‑1/CXCR4) axis. Accumulating studies have identified numbers of microRNAs (miRNAs) that serve important roles in pathogenesis OA. However, whether and how inhibition SDF‑1/CXCR4 axis induces alterations miRNA expression remains largely unclear. profiling was performed OA chondrocytes stimulated SDF‑1 alone, or CXCR4 antagonist TN14003 by...

Background: To explore the location accuracy and early clinical outcomes of using a 3D-printed individualized navigation template to assist in reconstruction anterior cruciate ligament (ACL). Methods: A single center randomized control study was conducted. Patients with ACL injury were treated conventional operation or an assisted by (the 3D group). The primary endpoint actual compared planned position. Results: There 20 23 participants group group, respectively. no differences bone tunnel...

Abstract Three kinds of reactive toughening agents bamboo plastic composites are studied in this article. The bio‐fiber keeps high polarity for the hydroxyl groups surface, while polypropylene (PP) matrix resin phase is nonpolar. So, interfacial compatibility between and enhanced poor. anhydride maleic grafted can react with hydroxyls. A large number on fiber surface reduced, bond strength improved. agents: glycidyl methacrylate poly(ethylene‐1‐octene), poly(ethylene‐octene),...

Abstract Background Animal model of Knee Osteoarthritis (OA) is the primary testing methodology for studies on pathogenic mechanisms and therapies human OA disease. Recent major modeling methods are divided into artificially induced spontaneous. However, these have some disadvantages slow progression, high cost no correlation with pathogenesis OA. Methods Our attempted to find a rapid, easy, consistent natural pathological process method by intra-articular injection stromal cell-derived...

Shuang Huang Lian Injection (SHLI) has been used in China for over 30 years as an effective and widely Chinese herbal prescription to treat acute respiratory infectious. SHLI has, however, caused many severe anaphylactoid reactions. It is important identify the potential components of SHLI. Spectrum-effect relationships were explore potentially components. Based on original formula, honeysuckle, Fructus Forsythiae Radix Scutellariae extracts prepared combined appropriate proportions. The...

Abstract This research explored the HPLC fingerprints of Hypericum attenuatum Choisy, which has anti-arrhythmic activity. was adopted to perform a determination chemical H. specimens acquired through seven distinct sources. The activity each sample obtained pharmacodynamics experiments in animals. A regression analysis and correlation were utilized calculate relationship peak pharmacological effectiveness with identified peak. Peaks numbered 5, 7, 13 14 fingerprint regarded as likely agents....

Receptor interacting protein kinase 1 (RIPK1)-mediated cell death, including apoptosis and necroptosis, belongs to programmed death. It has been reported that RIPK1-mediated necroptosis exists in lesions of cerebral hemorrhage (CH). Electroacupuncture, a treatment derived from traditional Chinese medicine, could improve neurological impairment patients with brain injury.To investigate the protective role cross electro-nape acupuncture (CENA) CH, clarify potential mechanism.CH rat models were...

Deep vein thrombosis (DVT) is an early postoperative complication. Thrombosis formation, which potentially life-threatening, seriously affects the rehabilitation of patients after surgery. We aimed to establish a C57 mouse model DVT and examine changes in expression Krüppel-like factor 15 (KLF15) endothelial nitric oxide synthase (eNOS) venous wall tissues, we also investigated regulatory relationship KLF15 eNOS thrombin-induced human umbilical cell (HUVEC) injury model.The was established...

This study aimed to investigate the effects of stromal cell-derived factor-1 (SDF-1) on biphasic ceramic-like biologic bone (BCBB) in vivo repair large segment defect rabbits.A large-segment radius model rabbits was constructed. In experimental group, BCBB with SDF-1 sustained-release system were implanted into site. Other three groups including normal control, autologous graft, and implantation without set. After surgery, general observation, X-ray radiography scoring, tissue section...

Abstract Background Varieties of animals were used to study osteoarthritis pathogenesis. The Diannan small-ear pig, which is native Yunnan, China, thought have an articular anatomy similar that humans and more likely be a source pathological tissues than other animals. aim this was determine whether animal can serve as effective model explore the role SDF-1/CXCR4 signaling pathway in development Osteoarthritis Methods Twenty-seven adult pigs randomly divided into three groups underwent Hulth...

To investigate the heterotopic osteogenesis of tissue engineered bone using co-culture system vascular endothelial cells (VECs) and adipose-derived stem (ADSCs) as seed cells.The partially deproteinized biological (PDPBB) was prepared by fibronectin combined with (PDPB). The ADSCs 18-week-old Sprague Dawley (SD) rats VECs cord blood full-term pregnant SD were isolated cultured. Three kinds constructed in vitro: PDPBB+VECs (group A), PDPBB+ADSCs B), PDPBB+co-cultured (VECs∶ADSCs 1∶1, group...

ABSTRACT BACKGROUND: Constipation is frequently encountered in patients undergoing brain tumor resection. has negative effects on daily living, well-being, and individuals' quality of life. We examined the impact acupuncture electroacupuncture (EA) stimulation postoperative constipation for METHODS: Patients resection (n = 150) were randomly divided into a nontreatment group, an EA group. Rome III Diagnostic Criteria, Cleveland Clinic Score, symptom assessment, Patient Assessment Quality...

Objective To investigate the short-term effectiveness of total knee arthroplasty (TKA) assisted by three-dimensional (3D) printing osteotomy navigation template. Methods A retrospective study was performed on 60 patients with osteoarthritis bewteen January 2016 and June 2017. Thirty cases underwent TKA 3D template (3D group) 30 conventional (conventional group). There no significant difference in gender, age, body mass index, surgical side, disease duration between 2 groups ( P>0.05). The...

Objective. To assess the clinical efficacy of auricular acupressure plus eye exercises in treatment adolescent pseudomyopia, to discover an easier and safer way treat pseudomyopia. Methods. Forty patients with pseudomyopia were randomly recruited assigned (1 : 1) receive either (single group, including 11 males 9 females) or (combined 12 8 females). The endpoint was after 28 days treatment. Results. baseline characteristics combined group comparable those single ( <a:math...

Introduction: Independent augmentation technology based on reinforcing devices has been reported to signifi-cantly reduce the elongation behavior of graft and improve knee stability after anterior cruciate ligament reconstruction (ACLR). Using biodegradable could risk severe inflammatory reactions due particle accumulation from foreign bodies. Given limitations mechanical properties materials, partially composite may offer a compromise strategy. Methods: Three types absorbable core-sheath...