A5061659417- Reproductive Biology and Fertility
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Reproductive System and Pregnancy
- Cancer-related gene regulation
- Renal and related cancers
- RNA Research and Splicing
- Ovarian function and disorders
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Effects and risks of endocrine disrupting chemicals
- Epigenetics and DNA Methylation
- HVDC Systems and Fault Protection
- Neurotransmitter Receptor Influence on Behavior
- Forensic Toxicology and Drug Analysis
- Genetics, Aging, and Longevity in Model Organisms
- Nuclear Receptors and Signaling
- Extracellular vesicles in disease
- Birth, Development, and Health
- Endometriosis Research and Treatment
- Plant and Fungal Species Descriptions
- 3D Printing in Biomedical Research
- Chemokine receptors and signaling
- Antioxidants, Aging, Portulaca oleracea
Nanjing Medical University
2016-2025
China Pharmaceutical University
2013-2025
Suzhou Municipal Hospital
2018-2025
State Key Laboratory of Reproductive Medicine
2013-2023
National Taiwan Normal University
2019
-methyladenosine (m
Although many reports show that various kinds of stem cells have the ability to recover function premature ovarian insufficiency (POI), few studies are associated with mechanism cell treatment POI. We designed this experimental study investigate whether human adipose cell-derived exosomes (hADSC-Exos) retain restore and how hADSC-Exos work in process. A POI mouse model was established granule (hGCs) collected from individuals were prepared assess therapeutic effects illuminate hADSCs curing...
The N6‐methyladenosine (m6A) modification plays a central role in epigenetic regulation of the mammalian transcriptome. m6A can be demethylated by fat mass‐ and obesity‐associated (FTO) protein α‐ketoglutarate‐dependent dioxygenase alkB homolog 5 (ALKBH5) protein. Much less is known about that whether content involved POI (premature ovarian insufficiency) disease. In this case‐controlled study, 69 53 tubal occlusion patients were recruited from reproduction centers our hospital. For animal...
Premature ovarian insufficiency (POI) is clinically irreversible in women aged over 40 years. Although numerous studies have demonstrated satisfactory outcomes of mesenchymal stem cell therapy, the underlying therapeutic mechanism remains unclear. Exosomes were collected from culture medium human umbilical cord cells (hUMSCs) and assessed by electron microscopy Western blot (WB) analysis. Then, exosomes added to cyclophosphamide (CTX)-damaged granulosa (hGCs), mixture was injected into...
Although many reports show that various kinds of stem cells have the ability to recover function in premature ovarian aging, few studies looked at cell treatment natural aging (NOA). We designed this experimental study investigate whether human amniotic mesenchymal (hAMSCs) retain restore function, and how hAMSCs work process. To build NOA mouse model, mice were fed for 12–14 months normally with young fertile female as normal control group (3–5 old). Hematoxylin eosin staining permitted...
Human amniotic mesenchymal stem cells (hAMSCs) were previously shown to effectively rescue ovarian function in a premature insufficiency (POI) mouse model. The therapeutic mechanism of hAMSC-derived exosomes (hAMSC-Exos) is not fully understood. In this study, the involved exosomal microRNA-320a (miR-320a) and Sirtuin 4 (SIRT4) was investigated POI ovaries oocytes human granulosa (hGCs) by fluorescence-activated cell sorting (FACS), hematoxylin eosin (H&E) staining, enzyme-linked...
Abstract Background Premature ovarian insufficiency (POI) is one of the major causes infertility. We previously demonstrated that transplantation menstrual blood-derived stromal cells (MenSCs) effectively improved function in a murine model POI. Recent studies indicated mesenchymal stem cell-derived exosomes were important components tissue repair. In this study, we investigated therapeutic effects MenSCs-derived (MenSCs-Exos) rat POI and its mechanism restoring ovulation. Methods Ovaries...
ABSTRACT Rationate The emergence of synthetic opioids represents a complex and concerning development in the field new psychoactive substances (NPSs). Nitazene analogs, also known as nitazenes or 2‐benzylbenzimidazole derivatives, represent recently emerging popular subgroup opioid receptor agonists. This study's streamlined approach aims to facilitate rapid accurate structural elucidation nitazene analogs. Method Ultra high‐performance liquid chromatography‐quadrupole time flight‐mass...
Many reports have shown that various kinds of stem cells the ability to recover premature ovarian aging (POA) function. Transplantation human amniotic epithelial (hAECs) improves function damaged by chemotherapy in a mice model. Understanding how evaluate distinct effects adult curing POA and choose clinical application is lacking. To build different degrees model, were administered doses cyclophosphamide: light dose (70 mg/kg, 2 weeks), medium 1 week; 120 week), high (120 weeks)....
Abstract Background With the development of regenerative medicine and tissue engineering technology, almost all stem cell therapy is efficacious for treatment premature ovarian failure (POF) or insufficiency (POI) animal models, whereas little has been practiced in clinical settings. The underlying molecular mechanism safety POI are not fully understood. In this study, we explored whether fetal mesenchymal cells (fMSCs) from liver restore function melatonin membrane receptor 1 (MT1) acts as...
Abstract Background Before starting gonadotoxic therapies, cryopreservation of mature sperm has been proposed worldwide as a method for male fertility preservation and enabling the conception healthy baby with assisted reproductive technology (ART); however, these technologies are not feasible prepubertal boys men spermatogenic failure. Transplantation mesenchymal stem cells exhibited successful therapeutic benefits in restoring spermatogenesis via gonadal graft angiogenesis, transplanted...
RNA modification-induced ovarian dysgenesis appears to be necessary for ovary development. However, how m5 C (5-methylcytosine)-coordinating modificatory transcripts are dynamically regulated during oogenesis, and development is unknown. The purpose of this study was determine whether NOP2/Sun methyltransferase 5 (Nsun5) deletion leads suppression function arrest embryonic regulation mRNA decay stability by modification essential at multiple stages the maternal-to-zygotic (MZT)...
Background/Aims: Human adipose-derived stem cells (hADSCs) are a potential therapeutic option for clinical applications because of their ability to produce cytokines and capacity trilineage differentiation. To date, few researchers have investigated the effects hADSCs on natural ovarian aging (NOA). Methods: An NOA mouse model human granule (hGCs) collected from individuals with were prepared assess illuminate mechanism in curing NOA. Enzyme-linked immunosorbent assay was used detect serum...
Cyclophosphamide (CTX) is one of the most frequently used alkylating anticancer drugs. CTX associated with reproductive failure and premature ovarian insufficiency (POI) or aging. Much less known about mechanism by which affects female fertility through N6-methyladenosine (m6A) levels. In this case-controlled study, we employed human granulosa cells mice as experimental models in vitro vivo. m6A test kit was developed to determine content RNA, qPCR western blot were examine expression levels...
Human placental mesenchymal stem cells (hPMSCs) have the ability to release cytokines and differentiate into three germ layers.To date, relevance of hPMSCs for treatment premature ovarian insufficiency (POI) disease through regulation oxidative stress is still unclear.Therefore, evaluate therapeutic efficiency investigate mechanism hPMSCs, we generated a mouse model POI collected human granule (hGCs) from patients with POI.hPMSCs displayed effects on function, including recovered follicular...
The side effects of busulfan on male reproduction are serious, so fertility preservation in children undergoing treatment is a major worldwide concern. Human placental mesenchymal stem cells (hPMSCs) have advantages such as stable proliferation and lower immunogenicity that make them an ideal material for stimulating tissue repair, especially restoring spermatogenesis. protective hPMSCs busulfan-induced Sertoli busulfan-treated mouse testes not been determined. Our study aimed to elaborate...
N6-methyladenosine (m6A) is the most prevalent reversible RNA modification in mammalian transcriptome. It has recently been demonstrated that m6A crucial for male germline development. Fat mass and obesity-associated factor (FTO), a known demethylase, widely expressed human mouse tissues involved manifold biological processes diseases. However, function of FTO spermatogenesis fertility remains poorly understood. Here, we generated an Fto knockout model using CRISPR/Cas9-mediated genome...
Abstract The versatile epigenetic modification known as N6‐methyladenosine (m 6 A) has been demonstrated to be pivotal in numerous physiological and pathological contexts. Nonetheless, the precise regulatory mechanisms linking m A histone modifications involvement of transposable elements (TEs) ovarian development aging are still not completely understood. First, we discovered that highly expressed during (OA), with significant contributions from decreased demethylase FTO overexpressed...
Journal Article Cohort Profile: The Jiangsu Birth Get access Jiangbo Du, Du State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, ChinaDepartment Epidemiology Biostatistics, School Public China Search for other works by this author on: Oxford Academic PubMed Google Scholar Yuan Lin, Lin Maternal, Child Adolescent Center Global Yankai Xia, Xia ChinaKey Modern Toxicology Ministry Education, https://orcid.org/0000-0003-0484-4035 Hongxia Ma, Ma...
The most effective method for mapping N6-methyladenosine (m6A) is m6A RNA immunoprecipitation sequencing (MeRIP-seq). quality of MeRIP-seq relies on various factors, with the anti-m6A antibody being a crucial determinant. However, comprehensive research selection and optimal concentrations different tissues has been limited. In this study, we optimized concentration five antibodies across tissues. Our findings demonstrated that 5 µg Millipore (ABE572 MABE1006) performed well, starting from...