A5027879419- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Contact Dermatitis and Allergies
- Asthma and respiratory diseases
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- Occupational exposure and asthma
- Eosinophilic Esophagitis
- Immunotherapy and Immune Responses
- Dermatology and Skin Diseases
- T-cell and B-cell Immunology
- Proteins in Food Systems
- Drug-Induced Adverse Reactions
- Mast cells and histamine
- Toxin Mechanisms and Immunotoxins
- Pediatric health and respiratory diseases
- Plant Reproductive Biology
- Delphi Technique in Research
- Plant tissue culture and regeneration
- Engineering and Materials Science Studies
- Insect and Pesticide Research
- Parasitic Infections and Diagnostics
- Advanced Proteomics Techniques and Applications
- Ginseng Biological Effects and Applications
- vaccines and immunoinformatics approaches
Medical University of Vienna
2014-2024
ENT and Allergy
2015
University of Vienna
1997-2004
Research Institute of General Pathology and Pathophysiology, the Russian Academy of Medical Sciences
2002-2004
Vienna General Hospital
1997-2004
Holon Institute of Technology
2004
University of Toronto
1999
The major birch pollen allergen, Bet v 1, is a member of the ubiquitous PR-10 family plant pathogenesis-related proteins. In recent years, number diverse proteins with low sequence similarity to 1 was identified. addition, determination structure revealed existence large superfamily structurally related this study, we aimed identify and classify all 1-related structures from Protein Data Bank sequences Uniprot database. Structural comparisons representative members already known protein...
Abstract Since the discovery of immunoglobulin E (IgE) as a mediator allergic diseases in 1967, our knowledge about immunological mechanisms IgE‐mediated allergies has remarkably increased. In addition to understanding immune response and clinical symptoms, allergy diagnosis management depend strongly on precise identification elicitors reaction. past four decades, innovations bioscience technology have facilitated production well‐defined, highly pure molecules for component‐resolved (CRD),...
Parvalbumins are the most important fish allergens. Polysensitization to various species is frequently reported and linked cross-reactivity of their parvalbumins. Studies on its association allergenicity purified natural parvalbumins from different still lacking. In addition, some studies indicate that dark muscled such as tuna less allergenic.Total protein extracts cod, whiff, swordfish, all eaten in Spain, were tested for IgE-binding properties with 16 allergic patients' sera Madrid. The...
Summary Background Profilins are cross‐reactive plant allergens responsible for multiple pollen sensitization and pollen‐associated food allergy. While it is assumed that profilins from different species immunologically equivalent, some studies suggest partial or even lacking IgE cross‐reactivity between certain profilins. Objective We aimed to obtain a semi‐quantitative assessment of the contributions conserved species‐specific epitopes binding Methods compared model structures timothy,...
Art v 1, the major pollen allergen of composite plant mugwort (Artemisia vulgaris) has been identified recently as a thionin-like protein with bulky arabinogalactan-protein moiety. A close relative mugwort, ragweed (Ambrosia artemisiifolia) is an important source in North America, and, since 1990, become growing health concern Europe well. Weed pollen-sensitized patients demonstrated IgE reactivity to apparently 29–31 kDa. This reaction could be inhibited by 1. The purified consisted...
Allergy diagnosis relying on the determination of specific IgE is frequently complicated by presence cross-reacting unclear clinical relevance. Particularly, anaphylactogenic activity directed to cross-reactive carbohydrate moieties glycoproteins from plants and invertebrates has been a matter debate. In this study, we present biochemical immunological characterization Api g 5, glycoprotein allergen celery with homology FAD containing oxidases. Carbohydrate analysis revealed glycans carrying...
Kiwifruit is a significant elicitor of allergy both in children and adults. Digestibility two kiwifruit allergens, actinidin (Act d 1) thaumatin-like protein 2), was assessed using an vitro digestion system that approximates physiological conditions with respect to the passage food through stomach into duodenum. Act 1 precipitated simulated gastric fluid at pH 2 aggregated proceeded slowly. The residual precipitate redissolved completely duodenal 6.5 partially digested. Forty percent...
Pru p 1 (a Bet v homologue) and 3 nonspecific lipid transfer protein; nsLTP) are major allergenic proteins in peach fruit, but differ their abundance stability. has low is highly labile was purified after expression as a recombinant protein Escherichia coli. abundant peel by conventional methods. The identities of the were confirmed sequence analysis masses determined MS analysis. reacted with antisera against related allergens from other species: antiserum to Mal d (from apple). presence...
Clinical reactions to bony fish species are common in patients with allergy and caused by parvalbumins of the β-lineage. Cartilaginous such as rays sharks contain mainly α-parvalbumins their allergenicity is not well understood.To investigate cartilaginous individuals allergic fish.Sensitization cod, salmon, ray among or both (n = 18) was explored prick-to-prick testing. reactivity assessed 11 food challenges clinical workup. IgE-binding β-parvalbumins (cod, carp, barramundi, tilapia) (ray,...
<i>Background:</i> Plant profilins are important pan-allergens. They responsible for a significant percentage of pollen-related allergies. Limited information is available about their involvement in the latex-fruit syndrome and cross-reactivities between latex pollen. We aimed to clone express <i>Hevea brasiliensis</i> profilin investigate its allergological significance serological from plant foods pollens. <i>Methods:</i> A DNA complementary messenger...
The stability of food allergens to proteolysis and thermal denaturation contributes considerably their allergenicity.Mal d 2, an allergenic thaumatin-like protein (TLP) from apple, was isolated purified by anion exchange chromatography. Its IgE reactivity tested ELISA immunoblotting using sera apple allergic patients. proteolytic Mal 2 investigated two in vitro digestion models. Effects treatment at different pH values on the secondary structure were recorded circular dichroism. level...
Background Allergy to kiwifruit is increasingly reported across Europe. Currently, the reliability of its diagnosis by measurement allergen-specific IgE with extracts or skin testing fresh fruits unsatisfying. Objective To evaluate usefulness a component-based allergen microarray for allergy in large group patients. Methods With an microarray, we measured specific and IgG4 levels panel nine allergens sera 237 individuals allergy. Sera from 198 allergic patients without served as controls....
Allergens from nuts frequently induce severe allergic reactions in sensitive individuals. The aim of this study was to elucidate the physicochemical characteristics natural Cor a 14, 2S albumin hazelnut.Cor 14 purified raw hazelnuts using combination precipitation and chromatographic techniques. protein analyzed gel electrophoresis, MS, far-UV circular dichroism (CD) analyses. immunoglobulin E (IgE) binding native, heat-treated, vitro digested studied. We identified two different isoforms...
BackgroundTo date, no safe allergen-specific immunotherapy for patients with peanut allergy is available. Previous trials were associated severe side effects.ObjectiveWe sought to determine the relative importance of conformational and linear IgE-binding epitopes major allergen Ara h 2 produce a hypoallergenic variant abolished anaphylactogenic activity.MethodsWild-type mutant lacking loops containing IgE produced in insect cells. Conformational removed by unfolding these proteins through...