A5083898639- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Contact Dermatitis and Allergies
- Cell Adhesion Molecules Research
- Advancements in Transdermal Drug Delivery
- Nitric Oxide and Endothelin Effects
- Ion Transport and Channel Regulation
- Cellular Mechanics and Interactions
- Hormonal Regulation and Hypertension
- Food Allergy and Anaphylaxis Research
- Caveolin-1 and cellular processes
- Force Microscopy Techniques and Applications
- Barrier Structure and Function Studies
- Estrogen and related hormone effects
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- HER2/EGFR in Cancer Research
- Skin Protection and Aging
- Sodium Intake and Health
- Electron Spin Resonance Studies
- Nanoparticle-Based Drug Delivery
- Eicosanoids and Hypertension Pharmacology
- Electrolyte and hormonal disorders
- Angiogenesis and VEGF in Cancer
- Nanoparticles: synthesis and applications
CeNTech
2012-2024
Community Living
2023
Deutschen Institute für Textil und Faserforschung
2021-2023
Institute of Textile Technology and Process Engineering
2016
University of Münster
2004-2015
In-Q-Tel
2015
Czech Academy of Sciences, Institute of Physiology
2007-2008
University of Würzburg
2004
University of Graz
1999-2000
Hôpital d'Hautepierre
1995
Dietary salt plays a major role in the regulation of blood pressure, and mineralocorticoid hormone aldosterone controls homeostasis extracellular volume. Recent observations suggest that small increase plasma sodium concentration may contribute to pressor response dietary salt. Because endothelial cells are (i) sensitive aldosterone, (ii) physical contact with sodium, (iii) crucial regulators vascular tone, we tested whether acute changes concentration, within physiological range, can alter...
In the presence of aldosterone, plasma sodium in high physiological range stiffens endothelial cells and reduces release nitric oxide. We now demonstrate effects extracellular potassium on stiffness individual cultured bovine aortic by using tip an atomic force microscope as a mechanical nanosensor. An acute increase swells softens cell increases A concentration, prevents these changes. propose that are caused submembranous cortical fluidization because actin depolymerization induced...
BackgroundLoss-of-function (LOF) mutations in the filaggrin gene (FLG) are a well-replicated risk factor for atopic dermatitis (AD) and known to cause an epidermal barrier defect. The nature of this defect is not fully understood. Patients with AD FLG LOF have more persistent disease, severe greater food allergies eczema herpeticum. Abnormalities corneocyte morphology been observed patients AD, including prominent villus-like projections (VP); however, these ultrastructural features...
Exposure of cells to colloidal nanoparticles (NPs) can have concentration-dependent harmful effects. Mostly, such effects are monitored with biochemical assays or probes from molecular biology, i.e., viability assays, gene expression profiles, etc., neglecting that the presence NPs also drastically affect cellular morphology. In case polymer-coated Au NPs, we demonstrate upon NP internalization, undergo lysosomal swelling, alterations in mitochondrial morphology, disturbances actin and...
MicroRNAs (miRNAs, micro ribonucleic acids) are pivotal post-transcriptional regulators of gene expression. These endogenous small non-coding RNAs play significant roles in tumorigenesis and tumor progression. miR-142-3p expression is dysregulated several breast cancer subtypes. We aimed at investigating the role cell invasiveness. Supported by transcriptomic Affymetrix array analysis confirmatory investigations mRNA protein level, we demonstrate that overexpression MDA-MB-231, MDA-MB-468...
There is currently no insight into biomarkers that can predict the onset of pediatric atopic dermatitis (AD).Nested in a prospective birth cohort study examined occurrence physician-diagnosed AD 300 children, 44 random children with first year life were matched on sex and season who did not develop AD. Natural moisturizing factor (NMF), corneocyte surface protrusions, cytokines, free sphingoid bases (SBs) different chain lengths their ceramides analyzed from tape strips collected at 2 months...
Aldosterone has long been known to control water and electrolyte balance by acting on mineralocorticoid receptors in kidney. However, recent studies demonstrated the presence of these nonclassical locations, including cardiovascular system. We tested hypothesis whether endothelial cells respond aldosterone with changes cell volume, a measure for ion-mediated movement across membrane. By means atomic force microscopy fluid, we measured volume adherent human umbilical venous exposed 72 hours...
Atopic dermatitis (AD) affects children of all skin types. Most research has focused on light Studies investigating biomarkers in people with AD dark types are lacking.To explore barrier and immune response stratum corneum (SC) tape strips from different types.Tape were collected lesional nonlesional forearm 53 50 controls. We analysed 28 immunomodulatory mediators, natural moisturizing factors (NMF) corneocyte morphology.Interleukin (IL)-1β, IL-18, C-X-C motif chemokine (CXCL) 8 (CXCL8),...
The action of glucocorticoids on vascular permeability is well established. However, little known about the mineralocorticoids structure and function blood vessels. As endothelial cells are targets for both mineralocorticoids, we exposed human umbilical vein to types steroids. Aldosterone (mineralocorticoid) dexamethasone (glucocorticoid) were applied 3 days in culture before measurements transendothelial ion macromolecule permeability, apical cell surface stiffness taken. Transendothelial...
AVP and atrial natriuretic peptide (ANP) have opposite effects in the kidney. induces antidiuresis by insertion of aquaporin-2 (AQP2) water channels into plasma membrane collecting duct principal cells. ANP acts as a diuretic factor. An ANP- nitric oxide (NO)/soluble guanylate cyclase (sGC)-induced AQP2 is reported from different models. However, functional data on missing. We used primary cultured inner medullary (IMCD) cells digital holographic microscopy, calcein-quenching measurements,...
Secreted microRNAs (miRNAs) reside in a complex regulatory network with extracellular matrix (ECM) macromolecules, which affect cell-cell communication, therefore miRNA expression highlights its significance several aspects of human diseases, including cancer. miRNA-mediated regulation breast cancer has received considerable attention due to evidence that shows miRNAs mediate estrogen receptor (ER) status, metastasis, chemoresistance and epithelial-to-mesenchymal transition (EMT). miR-200b...
Abstract Aim: In response to aldosterone endothelial cells swell and stiffen. Although amiloride‐sensitive sodium water uptake is known be involved, the underlying mechanisms are yet unclear. We tested hypothesis whether intracellular accumulation of or organic matter responsible for structural functional alterations. Methods: Atomic force microscopy was used as an imaging tool a mechanical nanosensor. Cell water, cell pressure measured at single level in human umbilical vein (HUVEC)....
Inflammation and cellular fibrosis often imply an involvement of the cytokine TGF-beta1. TGF-beta1 induces epithelial-to-mesenchymal transdifferentiation (EMT), a term describing loss epithelium-specific function. Indicative for this process are elongated cell shape parallel to stress fibre formation. Many signalling pathways have been discovered, but mechanical aspects not yet investigated. In study, atomic force microscopy (AFM) was used analyse surface topography properties EMT in...
Summary Background The irritant sodium lauryl sulfate ( SLS ) is known to cause a decrease in the stratum corneum level of natural moisturizing factor NMF ), which itself associated with changes corneocyte surface topography. Objective To explore this phenomenon allergic contact dermatitis. Methods Patch testing was performed on patients previously positive patch test reactions potassium dichromate C r), nickel N i), methylchloroisothiazolinone MCI )/methylisothiazolinone MI or p...
Background Recently, natural moisturizing factors (NMFs) and corneocyte surface topography were suggested as biomarkers for irritant dermatitis. Objectives To investigate how exposure to different irritants influences topography, NMF levels the barrier function of human skin in vivo. Methods Eight healthy adult volunteers exposed aqueous solutions 60% n‐propanol, 0.5% sodium lauryl sulfate (SLS), 0.15% hydroxide, 2.0% acetic acid, distilled water, a repeated irritation test over period 96...
(1) Background: The aim of the study was to evaluate effect pure lecithins in comparison a conventional surfactant on skin vivo. (2) Methods: Physiological parameters were evaluated at beginning and end (day 1 day 4) (n = 8, healthy forearm skin) with an Aquaflux®, skin-pH-Meter, Corneometer® Epsilon® sensor. Confocal Raman spectroscopy employed monitor natural moisturizing factor, urea water content participants’ skin. Tape strips treated sites taken collected corneocytes subjected atomic...
17beta-estradiol is known to delay the onset of atherosclerosis in women but cellular mechanisms are still unclear. Estrogens bind specific receptors and initiate a signaling cascade that involves activation plasma membrane Na(+)/H(+) exchange. We hypothesized estrogens interfere with ion transport across thus control endothelial structure function. Therefore, we investigated effects sex steroids 17beta-estradiol, progesterone, testosterone on volume, apical surface elasticity human...
During the winter in northern countries, risk of dermatitis is increased due to low temperature and humidity. Dermatitis particularly common on weather‐exposed skin such as cheeks hands. Recently, numbers unidentified nanosized protrusions corneocyte surface were associated with deficiency natural moisturizing factor (NMF). To investigate effect season NMF levels texture cheek hand healthy adults. Eighty volunteers (40 male 40 female) recruited: aged 18–40 years ≥ 70 years. Cheek dorsal was...
Silver is increasingly being used in garments to exploit its antibacterial properties. Information on the presence of silver nanoparticles (AgNPs) and their vivo penetration across healthy impaired skin from use limited. We investigated AgNPs a containing garment stratum corneum (SC) subjects (CTRLs) individuals with atopic dermatitis (AD). Seven CTRLs seven AD patients wore sleeve (13% Ag w/w) 8 h/day for five days forearm placebo other forearm. After days, layers SC were collected by...
Triple-negative breast cancer (TNBC) is defined by aggressive behavior, limited response to chemotherapy and lower overall survival rates. The increased metastatic potential of TNBC a combined result extensive extracellular matrix (ECM) remodeling that leads cytoskeleton rearrangement activation epithelial-to-mesenchymal transition (EMT). overexpression epidermal growth factor receptor (EGFR) in tumors has been linked induced expression EMT-related molecules. EMT often associated with...